Hostname: page-component-7479d7b7d-q6k6v Total loading time: 0 Render date: 2024-07-12T04:50:22.573Z Has data issue: false hasContentIssue false
  • English
  • Français

DNA syntheses in course of meiotic development in Neurospora crassa*†

Published online by Cambridge University Press:  14 April 2009

G. A. S. Iyengar ,
P. C. Deka ,
S. C. Kundu  and
S. K. Sen
G. A. S. Iyengar
Affiliation:
Botany Department, Bose Institute, Calcutta 700009, India
P. C. Deka
Affiliation:
Botany Department, Bose Institute, Calcutta 700009, India
S. C. Kundu
Affiliation:
Botany Department, Bose Institute, Calcutta 700009, India
S. K. Sen
Affiliation:
Botany Department, Bose Institute, Calcutta 700009, India

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Nuclear DNA syntheses have been measured microspectrophotometrically in course of meiotic nuclear development in Neurospora crassa. The purpose of the study has been to establish the timing of these DNA syntheses in relation to meiosis and in the formation of its final products in an ideal genetic material, where the products of the meiotic division can be recovered and identified. Spectrophotometric analysis has indicated that the last premeiotic DNA replication is completed before caryogamy; a postmeiotic DNA replication takes place at the tetrad of the nuclei level; and finally a second postmeiotic DNA replication occurs in the ascospores to result in eight binucleated ascospores. The timing of postmeiotic replication is compatible with the models explaining gene conversion through the postulated mechanisms involving heteroduplex formation.

Type
Research Article
Information
Genetics Research , Volume 29 , Issue 1 , February 1977 , pp. 1 - 8
Copyright
Copyright © Cambridge University Press 1977

References

REFERENCES

Barry, E. G. (1966). Cytological techniques for meiotic chromosomes in Neurospora. Neurospora Newsletter 10, 12–13.Google Scholar
Barry, E. G. (1967). Chromosome aberrations in Neurospora and the correlation of chromosomes and linkage group. Genetics 55, 21–32.CrossRefGoogle Scholar
Backett, A. & Wilson, I. M. (1968). Ascus cytology of Podospora anserina. Journal of General Microbiology 53, 81–97.CrossRefGoogle Scholar
El-Ani, A. S. (1956). Ascus development and nuclear behaviour in Hypomyces solani f. cucurbitae. American Journal of Botany 43, 769–778.CrossRefGoogle Scholar
Elliot, C. G. (1956). Chromosomes in microorganisms. Symposium Society of General Microbiology 6, 279–295.Google Scholar
Elliot, C. G. (1960). The cytology of Aspergillus nidulans. Genetical Research 1, 462–476.CrossRefGoogle Scholar
Emerson, S. (1969). Linkage and recombination at the chromosomal level. In Genetic Organization, vol. I (ed. Caspari, E. W. and Ravin, A. W.), pp. 267–360. New York: Academic Press.Google Scholar
Gillies, C. B. (1972). Reconstruction of the Neurospora crassa pachytene karyotype from serial sections of synaptonemal complexes. Chromosoma 36, 119–130.CrossRefGoogle ScholarPubMed
Grell, R. F. (1969). Meiotic and somatic pairing. In Genetic Organization, vol. I (ed. Caspari, E. W. and Ravin, A. W.), pp. 361–492. New York: Academic Press.Google Scholar
Holliday, R. (1964). A mechanism for gene conversion in fungi. Genetical Research 5, 282–304.CrossRefGoogle Scholar
Leuchtenberger, C. (1958). Quantitative determination of DNA in cells by Feulgen microspectrophotometry. In General Cytochemical Methods, vol. I (ed. Danielli, J. E.), pp. 219–278New York: Academic Press.Google Scholar
Lu, B. C. (1962). A new fixative and improved propionocarmine squash technique for staining fungus nuclei. Canadian Journal of Botany 40, 843–847.CrossRefGoogle Scholar
Lu, B. C. (1964). Chromosome cycles of the basidiomycete Cyatus stercoreus (Schw.) de Toni. Chromosoma 15, 170–178.CrossRefGoogle Scholar
McClintock, B. (1945). Neurospora. I. Preliminary observations of the chromosomes of Neurospora crassa. American Journal of Botany 32, 671–678.CrossRefGoogle Scholar
Minagawa, T., Wagner, B. & Strauss, B. (1959). The nucleic acid content of Neurospora crassa. Archives of Biochemistry and Biophysics 80, 442–445.CrossRefGoogle Scholar
Olive, L. S. (1965). Nuclear behaviour during meiosis. In The Fungi, vol. I (ed. Ains-worth, G. C. and Sussman, A. S.), pp. 143–161. New York: Academic Press.Google Scholar
Raju, N. B. & Lu, B. C. (1970). Meiosis in Coprinus. III. Timing of meiotic events in C. lagopus (sensu Buller). Canadian Journal of Botany 48, 2183–2186.CrossRefGoogle Scholar
Rossen, J. M. & Westergaard, M. (1966). Studies on the mechanism of crossing over. II. Meiosis and the time of meiotic chromosome replication in the ascomycetes Neottiella rutilans (Fr.). Comptes Rendus Des Travaux Du Laboratoire Carlsberg 35, 233–260.Google ScholarPubMed
Singleton, J. R. (1953). Chromosome morphology and the chromosome cycle in the ascus of Neurospora crassa. American Journal of Botany 40, 124–144.CrossRefGoogle Scholar
Sueoka, N., Chiang, K. S. & Kates, J. R. (1967). Deoxyribonucleicacid replication in meiosis of Chlamydomonas reinhardii. I. Isotopic transfer experiments with a strain producing eight zoospores. Journal of Molecular Biology 25, 47–66.CrossRefGoogle Scholar
Whitehouse, H. L. K. & Hastings, P. J. (1965). The analysis of genetic recombination on the polaron hybrid DNA model. Genetical Research 6, 27–92.CrossRefGoogle ScholarPubMed
Wilson, I. M. (1937). A contribution to the study of the nuclei of Peziza rutilans Fries. Annals of Botany 1, 655–671.CrossRefGoogle Scholar