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Disturbed haem and globin synthesis in reticulocytes of prenatal flexed-tailed (f/f) anaemic mice

Published online by Cambridge University Press:  14 April 2009

R. J. Cole ,
J. Garlick  and
R. G. Tarbutt
R. J. Cole
Affiliation:
Genetics and Development Group, School of Biological Sciences, University of Sussex, Brighton
J. Garlick
Affiliation:
Genetics and Development Group, School of Biological Sciences, University of Sussex, Brighton
R. G. Tarbutt
Affiliation:
Genetics and Development Group, School of Biological Sciences, University of Sussex, Brighton
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Haem synthetase activity and co-ordination of α- and β-globin chain synthesis have been investigated in prenatal reticulocytes of congenic FL/4Re +/+ Lvb/Lvb and FL/1 Ref/f Lvb/Lvb, mice, which have a marked hypochromic, microcytic, siderocytic anaemia, with reduced erythrocyte numbers at birth, and also in other stocks bearing the f lesion. Haem synthetase activity in f/f reticulocyte homogenates was similar to that in normal cells but was markedly dependent on protoporphyrin added to the homogenate, while activity in normal cell homogenates was relatively independent of added precursor. In cultured normal prenatal reticulocytes α- and β-globin was synthesized in approximately equal amounts during a 4 h labelling period, but in f/f reticulocytes there was an approximate 50% deficiency in β-globin chain synthesis. This deficiency could be repaired by added haem but not by protoporphyrin. Such a lesion is quantitatively consistent with the observed hypochromia of neonatal f/f erythrocytes. The relationship of this abnormality to effects of the f locus on early erythropoietic precursor cells is discussed.

Type
Research Article
Information
Genetics Research , Volume 23 , Issue 2 , April 1974 , pp. 125 - 135
Copyright
Copyright © Cambridge University Press 1974

References

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