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A correlation between development time and variegated position effect in Drosophila melanogaster

Published online by Cambridge University Press:  14 April 2009

John Michailidis ,
Neil D. Murray  and
Jennifer A. Marshall Graves
John Michailidis
Affiliation:
Department of Genetics and Human Variation, La Trobe University, Bundoora, Victoria 3083, Australia
Neil D. Murray
Affiliation:
Department of Genetics and Human Variation, La Trobe University, Bundoora, Victoria 3083, Australia
Jennifer A. Marshall Graves
Affiliation:
Department of Genetics and Human Variation, La Trobe University, Bundoora, Victoria 3083, Australia
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Position-effect variegation is a phenomenon in which cell-autonomous genes, normally expressed in all cells of a tissue, are expressed in some cells but not in others, leading to a mosaic tissue. Variegation occurs when a normally euchromatic gene is re-positioned close to heterochromatin by chromosome rearrangement. The extent of variegation is known to be influenced by a number of environmental and genetic factors. In the courss of investigations of the influence of the pH of larval medium on the extent of eye-colour variegation in In(1)ωm4 Drosophila melanogaster, we have found that the extent of variegation depends on development time. Flies reared at pH 2·6 develop slowly and show more extreme variegation than those reared at higher pH. This effect, as well as variations within the pH treatments, can be accounted for by differences in development time. The observed regression relationship between variegation and development time also appears to accommodate the influences of temperature on both variables. We suggest that development time may account causally for the reported influences of a number of environmental agents (temperature, crowding, chemicals) on variegation. Ways in which this might occur are discussed in the context of models of the molecular basis of differential gene activity.

Type
Research Article
Information
Genetics Research , Volume 52 , Issue 2 , October 1988 , pp. 119 - 123
Copyright
Copyright © Cambridge University Press 1988

References

Achwal, C. W., Ganguly, P. & Chandra, H. S. (1984). Estimation of the amount of 5-methylcytosine in Drosophilia melanogaster DNA by amplified ELISA and photoacoustic spectroscopy. EMBOJ. 3, 263–266.CrossRefGoogle Scholar
Baker, W. K. (1968). Position-effect variegation. Advances in Genetics 14, 133–169.CrossRefGoogle ScholarPubMed
Boffa, L. C., Gruss, R. J. & Allfrey, V. A. (1981). Manifold effects of sodium butyrate on nuclear functions. Journal of Biological Chemistry 256, 9612–9621.CrossRefGoogle Scholar
Cattanach, B. M. (1974). Position-effect variegation in the mouse. Genetical Research 23, 291–306.CrossRefGoogle ScholarPubMed
Christman, J. K., Weich, N., Schoenbrun, B., Schneider-man, N. K. & Acs, A. (1980). Hypomethylation of DNA during differentiation of Friend erythroleukemia cells. Journal of Cell Biology 86, 366–370.CrossRefGoogle ScholarPubMed
Cooper, K. W. (1959). Cytogenetic analysis of major heterochromatic elements (especially Xh and Y) in Drosophila melanogaster and the theory of ‘heterochromatin’. Chromosoma 10, 535–588.CrossRefGoogle ScholarPubMed
Demerec, M. & Slizynska, H. (1973). Mottled white 258–18 of Drosophila melanogaster. Genetics 25, 641–649.Google Scholar
Gartler, S. M., Dyer, K. A., Graves, J. A. M. & Rocchi, M. (1985). A two-step model for mammalian X chromosome inactivation. In Chemistry, Biochemistry and Biology of DNA Methylation (ed. Cantoni, G. L. and Razin, A.) pp. 223–235. New York; Alan R. Liss.Google Scholar
Gowen, J. W. & Gay, E. H. (1934). Chromosome constitution and behaviour in eversporting and mottling in Drosophila melanogaster. Genetics 19, 189–208.CrossRefGoogle ScholarPubMed
Hartmann-Goldstein, I. J. (1967). On the relationship between heterochromatinization and variegation in Drosophila with special reference to temperature-sensitive periods. Genetical Research 10, 143–159.CrossRefGoogle Scholar
Henikoff, S. (1981). Position-effect variegation and chromosome structure of a heat shock puff in Drosophila. Chromosoma 83, 381–393.CrossRefGoogle ScholarPubMed
Hinton, T. (1949). A correlation of phenotypic changes and chromosomal rearrangements at the two ends of an inversion. Genetics 35, 188–205.CrossRefGoogle Scholar
Holliday, R. (1987). X chromosome reactivation. Nature 327, 661–662.CrossRefGoogle ScholarPubMed
Judd, B. H. (1955). Direct proof of a variegated-type position effect at the white locus in Drosophila melanogaster. Genetics 40, 739–744.CrossRefGoogle ScholarPubMed
Mampell, K. (1965). Gene expression and development rate. Genetica 36, 135–146.CrossRefGoogle Scholar
Moore, G. D., Procunier, J. D., Cross, D. P. & Grigliatti, T. A. (1979). Histone gene deficiencies and position-effect variegation in Drosophila. Nature 282, 312–314.CrossRefGoogle ScholarPubMed
Moore, G. D., Sinclair, D. A. & Grigliatti, T. A. (1983). Histone gene multiplicity and position-effect variegation in Drosophila melanogaster. Genetics 105, 327–344.CrossRefGoogle ScholarPubMed
Mottus, R., Reeves, R. & Grigliatti, T. A. (1980). Butyrate suppression of position-effect variegation in Drosophila melanogaster. Molecular and General Genetics 78, 465–469.Google Scholar
Patel, C. V. & Gopinathan, K. P. (1987). Determination of trace amounts of 5-methylcytosine in DNA by reverse-phase high-performance liquid chromatography. Analytical Biochemistry 164, 164–169.CrossRefGoogle ScholarPubMed
Perrin, D. D. & Dempsey, B. (1974). Buffers for pH and Metal Ion Control, p. 176. London: Chapman and Hall.Google Scholar
Procunier, J. D. & Tartof, K. D. (1978). A genetic locus having trans and contiguous cis functions that control the disproportionate replication of ribosomal RNA genes in Drosophila melanogaster. Genetics 88, 67–79.CrossRefGoogle ScholarPubMed
Reuter, G., Werner, W. & Hoffman, H. J. (1982). Mutants affecting position-effect heterochromatinization in Drosophila melanogaster. Chromosoma 85, 539–551.CrossRefGoogle ScholarPubMed
Rushlow, C. A., Bender, W. & Chovnick, A. (1984). Studies on the mechanism of heterochromatic position effect at the rosy locus of Drosophila melanogaster. Genetics 108, 603–615.CrossRefGoogle ScholarPubMed
Saunders, D. S. (1982). Insect Clocks. 2nd Edn.New York: Pergamon Press.Google Scholar
Schultz, J. (1956). The relation of the heterochromatic chromosome regions to other nucleic acids of the cell. Cold Spring Harbor Symposia on Quantitative Biology 21, 307–328.CrossRefGoogle Scholar
Shoup, J. R. (1966). The development of pigment granules in the eyes of wild-type and mutant Drosphila melanogaster. Journal of Cell Biology 29, 223–249.CrossRefGoogle Scholar
Sinclair, D. A. R., Mottus, R. & Grigliatti, T. A. (1983). Genes which suppress position-effect variegation in Drosophila melanogaster are clustered. Molecular and General Genetics 191, 326–333.CrossRefGoogle Scholar
Spofford, J. B. (1976). Position-effect variegation in Drosophila. In The Genetics and Biology of Drosophila. (ed. Ashburner, M. and Novitski, E. Vol. lc, pp. 955–1018. Academic Press.Google Scholar
Urieli-Shoval, S., Gruenbaum, Y., Sedat, J. & Razin, A. (1982). The absence of detectable methylated bases in Drosophila melanogaster. FEBS Letters 146, 148–152.CrossRefGoogle ScholarPubMed
Wareham, K. A., Lyon, M. F., Glenister, P. H. & Williams, E. D. (1987). Age related reactivation of an X-linked gene. Nature 327, 725–727.CrossRefGoogle ScholarPubMed