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Copper localization in the duodenal mucosa of heterozygous tortoiseshell (Moto/+) female mice (Mus musculus)

Published online by Cambridge University Press:  14 April 2009

Harold J. Sheedlo  and
Melvin L. Beck
Harold J. Sheedlo
Affiliation:
Department of Biology, Memphis State University, Memphis, Tennessee 38152
Melvin L. Beck
Affiliation:
Department of Biology, Memphis State University, Memphis, Tennessee 38152

Summary

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The duodenal mucosa of normal (+/+) and heterozygous tortoiseshell (Moto / +) female mice was analysed by an ultrastructural histochemical copper localization technique. Copper was rarely detected along the duodenal microvilli of + / + female mice. However, copper was localized on the surface of the brush border of the duodenal mucosa and within pinocytotic vesicles at the base of the microvilli of Moto /+ female mice. The mottled defect may involve a defective intestinal uptake of copper, as well as a faulty copper transport mechanism.

Type
Short Paper
Information
Genetics Research , Volume 38 , Issue 3 , December 1981 , pp. 333 - 336
Copyright
Copyright © Cambridge University Press 1981

References

REFERENCES

Camakaris, J., Mann, T. R. & Danks, D. M. (1979). Copper metabolism in mottled mutants. Biochemical Journal 180, 597–604.CrossRefGoogle Scholar
Crampton, R. F., Matthews, D. M. & Poisner, R. (1965). Observations on the mechanism of absorption of copper by the small intestine. Journal of Physiology 178, 111–126.CrossRefGoogle ScholarPubMed
Dickie, M. M. (1954). The tortoise shell house mouse. Journal of Heredity 45, 158, 190.CrossRefGoogle Scholar
Horn, N. & Jensen, O. A. (1980). Menkes' syndrome; subcellular distribution of copper determined by an ultrastructural histochemical technique. UltrastructuralPathology 1, 237–242.Google ScholarPubMed
Hunt, D. M. (1974). Primary defect on copper transport underlies mottled mutants in the mouse. Nature 249, 852–854.CrossRefGoogle ScholarPubMed
Hunt, D. M. (1976). A study of copper treatment and tissue copper levels in the murine congenital copper deficiency, mottled. Life Sciences 19, 1913–1920.CrossRefGoogle ScholarPubMed
Hunt, D. M. & Port, A. E. (1979). Trace element binding in the copper deficient mottled mutants in the mouse. Life Sciences 24, 1453–1466.CrossRefGoogle ScholarPubMed
Johnson, W. T. & Evans, G. W. (1980). Isolation of a (copper, zinc)-thionein from the small intestine of neonatal rats. Biochemical and Biophysical Research Communications 96, 10–17.CrossRefGoogle ScholarPubMed
Lane, P. W. (1960). Mouse News Letter 23, 36.Google Scholar
Mann, J. R., Camakaris, J. & Danks, D. M. (1980). Copper metabolism in mottled mouse mutants. Biochemical Journal 186, 629–631.CrossRefGoogle ScholarPubMed
Prins, H. W. & Van Den Hamer, C. J. A. (1979). Primary biochemical defect in copper metabolism in mice with a recessive X-linked mutation analogous to Menkes' disease in man. Journal of Inorganic Biochemistry 10, 19–27.CrossRefGoogle ScholarPubMed
Prins, H. W. & Van Den Hamer, C. J. A. (1980). Abnormal copper-thionein synthesis and impaired copper utilization in mutated brindled mice: model for Menkes' disease. Journal of Nutrition 110, 151–157.CrossRefGoogle ScholarPubMed
Scheuer, P. J., Thorpe, M. E. C. & Marriott, P. (1967). A method for the demonstration of copper under the electron microscope. Journal of Histochemistry and Cytochemistry 15, 300–301.CrossRefGoogle ScholarPubMed
Silvers, W. K. (1979). The Coat Colors of Mice, pp. 159–184. New York: Springer-Verlag.CrossRefGoogle Scholar
Venable, J. H. & Coggeshall, H. (1965). A simplified lead citrate strain for use in electron microscopy. Journal of Cell Biology 25, 407–408.CrossRefGoogle Scholar