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Chromosomal distribution of the major insert in Drosophila melanogaster 28S rRNA genes

Published online by Cambridge University Press:  14 April 2009

W. J. Peacock ,
R. Appels ,
S. Endow  and
D. Glover
W. J. Peacock
Affiliation:
CSIRO, Division of Plant Industry, PO Box 1600, Canberra, ACT, Australia
R. Appels
Affiliation:
CSIRO, Division of Plant Industry, PO Box 1600, Canberra, ACT, Australia
S. Endow
Affiliation:
Dept. of Microbiology and Immunology, Duke University Medical Centre, Durham, North Carolina, U.S.A
D. Glover
Affiliation:
Department of Biochemistry, Imperial College, London, England

Summary

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The major type I insert sequence for the 28S rRNA genes of Drosophila melanogaster has been mapped within the chromosomes using a probe synthesized from a cloned sequence containing the entire 5·4 kb segment. The genomic distribution was shown to be complex in that the insert sequence occurred next to many different types of sequences, in addition to occurring as an insert in the 28S rRNA genes of the X chromosome. In situ hybridization of mitotic chromosomes showed most of the insert units not contained in the ribosomal genes to be located near the ribosomal gene cluster on the X chromosome. Additional sites were detected in polytene chromosomes in region 102C, 8–12 and in the hetero-chromatin of the autosomes.

Type
Short Paper
Information
Genetics Research , Volume 37 , Issue 2 , April 1981 , pp. 209 - 214
Copyright
Copyright © Cambridge University Press 1981

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