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Characterization of an antibiotic resistance plasmid pAV5 and its constituent replicons in Acinetobacter calcoaceticus

Published online by Cambridge University Press:  14 April 2009

Mark Divers ,
Edward Hinchliffe  and
Alan Vivian
Mark Divers
Affiliation:
School of Biological Sciences and Environmental Health, Thames Polytechnic, Wellington Street, London, SE18 6PF
Edward Hinchliffe
Affiliation:
School of Biological Sciences and Environmental Health, Thames Polytechnic, Wellington Street, London, SE18 6PF
Alan Vivian
Affiliation:
School of Biological Sciences and Environmental Health, Thames Polytechnic, Wellington Street, London, SE18 6PF

Summary

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A non-transmissible plasmid, pAV5, was isolated from a hospital strain of Acinetobacter calcoaceticus, JC17. pAV5 confers resistance to the antibiotics tetracycline and neomycin in the genetically characterized strain of A. calcoaceticus EBF 65/65. Transfer of pAV5 can be mediated by the sex factors pAV1 and R751; transfer is occasionally associated with the segregation of the resistance determinants amongst the transconjugants. Phenotypic dissociation of pAV5 corresponds with the formation of two independent plasmids designated pAV51 and pAV52 mediating resistance to neomycin and tetracycline respectively.

Type
Research Article
Information
Genetics Research , Volume 44 , Issue 1 , August 1984 , pp. 1 - 9
Copyright
Copyright © Cambridge University Press 1984

References

REFERENCES

Broome-Smith, J. (1980). RecA independent, site-specific recombination between Col EI or Col K and a miniplasmid they complement for mobilization and relaxation: implications for the mechanism of DNA transfer during mobilization. Plasmid 4, 51–63.CrossRefGoogle Scholar
Eckhardt, T. (1978). A rapid method for the identification of plasmid deoxyribonucleic acid in bacteria. Plasmid 1, 584–588.CrossRefGoogle ScholarPubMed
Hinchliffe, E. & Vivian, A. (1980 a). Naturally occurring plasmids in Acinetobacter calcoaceticus: pAV1, a P class R factor of restricted host range. Journal of General Microbiology 116, 75–80.Google Scholar
Hinchliffe, E. & Vivian, A. (1980 b). Gene transfer in Acinetobacter calcoaceticus: fertility variants of the sex factor pAV1. Journal of General Microbiology 119, 117–122.Google ScholarPubMed
Hinchliffe, E. & Vivian, A. (1980 c). Restriction mediated by pAV2 affects the transfer of plasmids in Acinetobacter calcoaceticus. Journal of General Microbiology 121, 419–423.Google ScholarPubMed
Hinchliffe, E., Nugent, M. E. & Vivian, A. (1980). Naturally occuring plasmids in Acinetobacter calcoaceticus: pAV2, a plasmid which influences the fertility of the sex factor pAV1. Journal of General Microbiology 121, 411–418.Google ScholarPubMed
Jobanputra, R. S. & Datta, N. (1974). Trimethoprim resistance factors in enterobacteria from clinical specimens. Journal of Medical Microbiology 7, 169–177.CrossRefGoogle ScholarPubMed
Juni, E. (1978). The genetics and physiology of Acinetobacter calcoaceticus. Annual Review of Microbiology 32, 349–371.CrossRefGoogle Scholar
Juni, E. & Janik, A. (1969). Transformation of Acinetobacter calcoaceticus (Bacterium anitratum). Journal of Bacteriology 98, 281–288.CrossRefGoogle ScholarPubMed
Ramphal, R. & Kluge, R. M. (1979). Acinetobacter calcoaceticus variety anitratus: an increasing nosocomial problem. American Journal of the Medical Sciences 277, 57–66.CrossRefGoogle ScholarPubMed
Towner, K. J. & Vivian, A. (1976 a). RP4-mediated conjugation in Acinetobacter calcoaceticus. Journal of General Microbiology 93, 355–360.CrossRefGoogle ScholarPubMed
Towner, K. J. & Vivian, A. (1976 b). RP4 fertility variants in Acinetobacter calcoaceticus. Genetical Research 28, 301–306.CrossRefGoogle ScholarPubMed
Vivian, A., Hinchliffe, E. & Fewson, C. A. (1981) Acinetobacter calcoaceticus: approaches to a problem. Journal of Hospital Infection 2, 199–203.CrossRefGoogle ScholarPubMed