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The characteristics of eleven mutants of R-factor R57 constitutive for tetracycline resistance, selected and tested in Escherichia coli K12

Published online by Cambridge University Press:  14 April 2009

E. C. R. Reeve  and
J. M. Robertson
E. C. R. Reeve
Affiliation:
Institute of Animal Genetics, West Mains Road, Edinburgh EH9 3JN, Scotland
J. M. Robertson
Affiliation:
Institute of Animal Genetics, West Mains Road, Edinburgh EH9 3JN, Scotland

Summary

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Eleven mutants of R-factor R57 have been isolated which show constitutive expression of resistance to tetracycline (Tc). These derepressed (Tdr) mutants all gave a much greater resistance to Tc and to its analogue, minocycline, than could be obtained by optimal induction of cells carrying the wild-type (T+) determinant. Cells carrying each of the Tdr mutants together with T+ of either R6-S or of a plasmid found in Escherichia coli mi19 showed inducible Tc resistance, indicating that the Tdr mutants were all recessive, i.e. of repressor-negative type. Tdr1 was not recessive to the T-determinant of RP1, suggesting that the repressor gene products of the T-determinants in R57 and RP1 have different specificities.

Type
Research Article
Information
Genetics Research , Volume 25 , Issue 3 , June 1975 , pp. 297 - 311
Copyright
Copyright © Cambridge University Press 1975

References

REFERENCES

Beckwith, J. & Zipser, D. (eds.) (1970). The lactose operon. Cold Spring Harbor Laboratory, New York.Google Scholar
Datta, N. & Hedges, R. W. (1972). R factors identified in Paris, some conferring gentamycin resistance, constitute a new compatibility group. Annales de L'Institut Pasteur 123, 849–852.Google ScholarPubMed
Embden, J. van & Cohen, S. N. (1973). Molecular and genetic studies of an R-factor system consisting of independent transfer and drug resistant plasmids. Journal of Bacteriology 116, 699–709.CrossRefGoogle Scholar
Fisher, R. A. & Yates, F. (1957). Statistical Tables for Biological, Agricultural and Medical Research, 5th ed.Edinburgh: Oliver and Boyd.Google Scholar
Foster, T. J. & Shaw, W. V. (1973). Chloramphenicol acetyltransferases specified by fi − R factors. Antimicrobial Agents and Chemotherapy 3, 99–104.CrossRefGoogle Scholar
Foster, T. J. & Walsh, A. (1975). Phenotypic characterisation of R-factor tetracycline resistance determinants. Genetical Research 24, 333–343.CrossRefGoogle Scholar
Franklin, T. J. (1967). Resistance of Escherichia coli to tetracyclines. Changes in permeability in Escherichia coli bearing transferable resistance factors. Biochemical Journal 105, 371–378.CrossRefGoogle ScholarPubMed
Franklin, T. J. & Cook, J. M. (1971). R-factor with a mutation in the tetracycline resistance marker. Nature 229, 273–274.CrossRefGoogle ScholarPubMed
Grinsted, J., Saunders, J. R., Ingram, L. C., Sykes, R. B. & Richmond, M. H. (1972). Properties of an R factor which originated in Pseudomonas aeruginosa 1822. Journal of Bacteriology 10, 529–537.CrossRefGoogle Scholar
Hoekstra, W. P. M., Zuidweg, E. M. & Kipp, J. B. A. (1973). Tetracycline-resistance in Escherichia coli: a genetic contribution. Antonie van Leeuwenhoek Journal of Microbiology and Serology 39, 11–20.CrossRefGoogle ScholarPubMed
Izaki, K., Kiuchi, K. & Arima, K. (1966). Specificity and mechanism of tetracycline resistance in a multiple drug-resistant strain of Escherichia coli. Journal of Bacteriology 91, 628–633.CrossRefGoogle Scholar
Levy, S. B. & McMurry, L. (1974). Detection of an inducible membrane protein associate with R-factor-mediated tetracycline resistance. Biochemical & Biophysical Research Communications 56, 1060–1068.CrossRefGoogle ScholarPubMed
Reeve, E. C. R. (1966). Characteristics of some single step mutants to chloramphenicol resistance in Escherichia coli K12 and their interactions with R-factor genes. Genetical Research 7, 281–286.CrossRefGoogle ScholarPubMed
Robertson, J. M. & Reeve, E. C. R. (1972). Analysis of the resistance mediated by several R-factors to tetracycline and minocycline. Genetical Research 20, 239–252.CrossRefGoogle ScholarPubMed
Witchitz, J. L. & Chabbert, Y. A. (1971). Résistance transférable à la gentamicine. II. Transmission et liaisons du caractère de résistance. Annales de L'Institut Pasteur 122, 367–378.Google Scholar