Hostname: page-component-848d4c4894-x24gv Total loading time: 0 Render date: 2024-06-05T10:56:28.416Z Has data issue: false hasContentIssue false
  • English
  • Français

The bearing of mutant and cross specificity on the pattern of intragenic recombination

Published online by Cambridge University Press:  14 April 2009

A. Paszewski  and
W. Prazmo
A. Paszewski
Affiliation:
Department of General Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
W. Prazmo
Affiliation:
Department of General Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Four white-spored allelic mutants of Ascobolus immersus were used to study the effect of mutant and cross specificity on the recombination pattern in intragenic crosses.

In the locus studied no correlation was found between the position of mutants on the map and their basic conversion frequencies. One of the mutants evidently caused an increase of conversion frequency of the two others. Crossing-over in intragenic recombination may be mutant-specific as revealed by using two mutants which give no recombinants when crossed with each other. The frequency of crossing-over was higher in crosses in repulsion than in coupling involving the same pairs of mutants. Polarization observed in two-point crosses was due in some instances predominantly to differences in basic conversion frequencies of the mutants used, and in others to the relative position of the mutated sites. Mutants located in the central part of the studied region were those which converted most frequently in two-point crosses. No reciprocal conversions were observed.

A number of recombinant asci resulting from two or more separate but highly correlated recombinational events within a gene were found.

Type
Research Article
Information
Genetics Research , Volume 14 , Issue 1 , August 1969 , pp. 33 - 43
Copyright
Copyright © Cambridge University Press 1969

References

REFERENCES

Fields, W. G. & Olive, L. S. (1967). The genetic of Sordaria brevicollis. III. Gene conversion involving a series of hyaline ascospore color mutants. Genetics 57, 483–493.CrossRefGoogle Scholar
Holliday, R. (1964). A mechanism for gene conversion in fungi. Genet. Res. 5, 282–304.CrossRefGoogle Scholar
Kruszewska, A. & Gajewski, W. (1967). Recombination within the Y locus in Ascobolus immersus. Genet. Res., Camb. 9, 159–177.CrossRefGoogle Scholar
Mousseau, J. (1963). On the possibility of carrying out the functional test for allelism in the Ascomycete Ascobolus immersus. Proc. XIth Int. Cong. Genet. 1–16.Google Scholar
Mousseau, J. (1966). Sur les variations de fréquence de conversion au niveau de divers sites d'un même locus. C. r. hebd. Séanc. Acad. Sci., Paris 262, 1254–1257.Google ScholarPubMed
Mousseau, J. (1967). Analyse de la structure fine d'un gène chez Ascobolus immersus. Contribution a l'étude de la recombinaison méiotique. Ph.D. thesis, University of Paris.Google Scholar
Paszewski, A. (1965). Konwersja genów i crossing-over u Ascobolus immersus. Ph.D. thesis, University of Warsaw.Google Scholar
Paszewski, A. (1967). A study on simultaneous conversions in linked genes in Ascobolus immersus. Genet. Res., Camb. 10, 121–126.CrossRefGoogle Scholar
Paszewski, A., Surzycki, S. & Mankowska, M. (1966). Chromosome maps in Ascobolus immersus (Rizet's strain). Acta Soc. Bot. Pol. 35, 181–188.CrossRefGoogle Scholar
Rossignol, J. L. (1967). Contribution a l'étude des phénomènes de de recombinaison intra-génique. Ph.D. thesis, University of Paris.Google Scholar
Whitehouse, H. K. L. (1966). An operator model of crossing-over. Nature, Lond. 211, 708–713.CrossRefGoogle ScholarPubMed
Whitehouse, H. K. L. (1967). Secondary crossing-over. Nature, Lond. 215, 1352–1359.CrossRefGoogle ScholarPubMed
Whitehouse, H. K. L. & Hastings, P. J. (1965). The analysis of genetic recombination on the polaron hybrid DNA model. Genet. Res., Camb. 6, 27–92.CrossRefGoogle ScholarPubMed