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Tunisian fig (Ficus carica L.) genetic diversity and cultivar characterization using microsatellite markers

Published online by Cambridge University Press:  15 July 2005

Olfa Saddoud ,
Amel Salhi-Hannachi ,
Khaled Chatti ,
Messaoud Mars ,
Abdelmajid Rhouma ,
Mohamed Marrakchi  and
Mokhtar Trifi
Olfa Saddoud
Affiliation:
Laboratoire de Génétique moléculaire, immunologie et biotechnologie, Faculté des Sciences de Tunis, Campus universitaire, 2092 El Manar Tunis, Tunisia
Amel Salhi-Hannachi
Affiliation:
Laboratoire de Génétique moléculaire, immunologie et biotechnologie, Faculté des Sciences de Tunis, Campus universitaire, 2092 El Manar Tunis, Tunisia
Khaled Chatti
Affiliation:
Laboratoire de Génétique moléculaire, immunologie et biotechnologie, Faculté des Sciences de Tunis, Campus universitaire, 2092 El Manar Tunis, Tunisia
Messaoud Mars
Affiliation:
École supérieure d’Horticulture et d’élevage, 4042 Chott Mariem, Sousse, Tunisia
Abdelmajid Rhouma
Affiliation:
IPGRI, Centre de Recherches phoénicicoles (CRPh), Degache, Tunisia
Mohamed Marrakchi
Affiliation:
Laboratoire de Génétique moléculaire, immunologie et biotechnologie, Faculté des Sciences de Tunis, Campus universitaire, 2092 El Manar Tunis, Tunisia
Mokhtar Trifi
Affiliation:
Laboratoire de Génétique moléculaire, immunologie et biotechnologie, Faculté des Sciences de Tunis, Campus universitaire, 2092 El Manar Tunis, Tunisia
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Abstract

Introduction. Ficus carica L., well adapted to the Mediterranean climate, is ubiquitous in Tunisia. In spite of the large possibilities of its adaptation to the Tunisian climate, its cultivation remains traditional. In Tunisia, this species is represented by a large number of varieties which are facing genetic erosion. To save these genetic resources, we studied some of the Tunisian varieties using molecular markers. The aim of this analysis was to study the genetic diversity of some cultivars and to characterize them. Materials and methods. Six microsatellites were used to characterize 16 cultivars (Ficus carica L.) belonging to two fig tree collections in the south of Tunisia. Results. The molecular markers used appeared highly polymorphic in common fig trees since 4–12 alleles per locus and a mean of heterozygoty of 0.656 were scored. The resolving power (Rp) of the six microsatellites tested ranged from 2.12 to 3.87 for the 16 cultivars studied, showing a significant genetic diversity (Ht = 0.762). Genetic differentiation between geographical groups was low (Gst = 0.032). The factorial correspondence analysis showed no well-defined relation between the 16 cultivars and their geographical origin. The genotype patterns allowed us to discriminate all of the cultivars. Conclusion. The characterization of the accessions belonging to different varieties was possible, showing the power and efficiency of the molecular tools used.

Keywords

TunisiaFicus caricagenetic resourcesidentificationmicrosatellitesgenetic markersTunisieFicus caricaressource génétiqueidentificationmicrosatellitemarqueur génétique
Type
Research Article
Information
Fruits , Volume 60 , Issue 2 , March 2005 , pp. 143 - 153
Copyright
© CIRAD, EDP Sciences

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