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A laboratory method to evaluate the sensitivity of Colletotrichummusae to postharvest fungicides

Published online by Cambridge University Press:  17 July 2008

Luc de Lapeyre de Bellaire  and
Yolande Chilin-Charles
Luc de Lapeyre de Bellaire
Affiliation:
CIRAD, Persyst, UPR, Systèmes bananes et ananas, CARBAP, BP 832, Douala, Cameroon
Yolande Chilin-Charles
Affiliation:
CIRAD, Bios, UPR, Multiplication végétative, Station de Neufchâteau, 97130, Capesterre Belle-Eau, Guadeloupe, France ; yolande.chilin-charles@cirad.fr
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Abstract

Introduction. This protocol aims at detecting and evaluating sensitivity shifts in Colletotrichum musae populations towards fungicides that are currently used for the control of postharvest diseases of bananas. The principle, key advantages, starting plant material, time required and expected results are presented. Materials and methods. The necessary laboratory materials and details of the twelve steps of the protocol for banana sampling and anthracnose lesion development, the agar plate preparation, single-spore isolation, germination test, and data analysis are described. Possible troubleshooting is mentioned. We obtain (a) for antimitotic products, the percentage of resistant strains in the population analysed; (b) for Sterol Biosynthesis Inhibitor (SBI) fungicides, the variation and distribution of the isolates' sensitivity within the population analysed according to their level of radial growth reduction by the fungicide.

Keywords

France (Guadeloupe)Musa sp.chemical controlmethodsColletotrichum musaeresistance to chemicalsFrance (Guadeloupe)Musa sp.lutte chimiqueméthodeColletotrichum musaerésistance aux produits chimiques
Type
Research Article
Information
Fruits , Volume 63 , Issue 4 , July 2008 , pp. 263 - 266
Copyright
© CIRAD, EDP Sciences, 2008

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References

De Lapeyre de Bellaire, L., Dubois, C., Distribution of thiabendazole-resistant Colletotrichum isolates from Guadeloupe banana plantations, Plant Dis. 81 (1997) 1378–1383. CrossRef
De Lapeyre de Bellaire, L., Chillet, M., Dubois, C., Mourichon, X., Importance of different sources of inoculum and dispersal methods of conidia of Colletotrichum musae, the causal agent of banana anthracnose, for fruit contamination, Plant Pathol. 49 (2000) 782–790. CrossRef
De Lapeyre de Bellaire L., Bio-écologie de Colletotrichum musae (Berk. & Curt.) Arx, agent de l'anthracnose des bananes, dans les conditions tropicales humides de la Guadeloupe, thèse, Univ. Paris-Sud, Orsay, France, 1999, 100 p.