Hostname: page-component-848d4c4894-cjp7w Total loading time: 0 Render date: 2024-07-03T15:10:21.574Z Has data issue: false hasContentIssue false
  • English
  • Français

Biochemical characterization of pulp of banana fruit: measurement of soluble sugars, organic acids, free ACC and in vitro ACC oxidase

Published online by Cambridge University Press:  15 May 2008

Bernard Fils-Lycaon ,
Didier Mbéguié-A-Mbéguié ,
Marc Chillet ,
Philippe Julianus ,
Colette Galas ,
Rose-Marie Gomez  and
Olivier Hubert
Bernard Fils-Lycaon
Affiliation:
INRA, UMR 1270 Qualitrop, Domaine de Duclos, Prise d’eau, 97170 Petit-Bourg, Guadeloupe, France
Didier Mbéguié-A-Mbéguié
Affiliation:
CIRAD-Persyst, UMR 94 Qualitrop, Station de Neufchâteau, Sainte-Marie, 97130 Capesterre-Belle-Eau, Guadeloupe, Francembeguie@cirad.fr
Marc Chillet
Affiliation:
CIRAD-Persyst, UMR 95 Qualisud, Univ. São Paulo, Av. Prof Lineu Prestes 580, Bloco 14, 05508-900, São Paulo, Brasilmarc.chillet@cirad.fr
Philippe Julianus
Affiliation:
INRA, UMR 1270 Qualitrop, Domaine de Duclos, Prise d’eau, 97170 Petit-Bourg, Guadeloupe, France
Colette Galas
Affiliation:
INRA, UMR 1270 Qualitrop, Domaine de Duclos, Prise d’eau, 97170 Petit-Bourg, Guadeloupe, France
Rose-Marie Gomez
Affiliation:
INRA, UMR 1270 Qualitrop, Domaine de Duclos, Prise d’eau, 97170 Petit-Bourg, Guadeloupe, France
Get access

Abstract

Introduction. We present some protocols aiming at partially characterizing banana fruit quality through measurement of some key biochemical parameters. The principle, key advantages, starting plant material, time required and expected results are presented. Materials and methods. This part describes the required laboratory materials and the steps necessary for achieving four protocols making it possible to measure sugar, organic acids and free ACC contents, and in vitro ACC oxidase activity. Results. Standard results obtained by using the protocols described are presented in the figures.

Keywords

FranceMusa sp.methodsfruitsmeasurementqualityFranceMusa sp.méthodefruitsmesurequalité
Type
Research Article
Information
Fruits , Volume 63 , Issue 3 , May 2008 , pp. 187 - 191
Copyright
© CIRAD, EDP Sciences, 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Célestine-Myrtil, D., Parfait, A., HPLC analysis of sugars in cane stalks, Int. Sugar J. 89 (1987) 186–220.
Célestine-Myrtil, D., Parfait, A., HPLC determination of organic acids in sugar cane and its industrial by-products, Int. Sugar J. 90 (1988) 28–32.
Lizada, M., Yang, S.A., Simple and sensitive assay for 1-aminocyclopropane-1-carboxylic acid, Anal. Biochem. 100 (1979) 140–145.
Liu, X., Shiomi, S., Nakatsuka, A., Kubo, Y., Nakamura, R., Inaba, A., Characterization of ethylene biosynthesis associated with ripening in banana fruit, Plant Physiol. 121 (1999) 1257–1265. CrossRef