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The role of cholinergic and peptidergic pathways in the regulation of pancreatic exocrine function during postnatal development in pigs

Published online by Cambridge University Press:  31 July 2001

V. Lesniewska ,
A. Gregard ,
B. Weström ,
M. S. Hedemann ,
H. N. Lærke ,
D. Kruszewska  and
S. G. Pierzynowski
V. Lesniewska
Affiliation:
Department of Animal Physiology, Faculty of Veterinary Medicine, Warsaw Agricultural University, ul. Nowoursynowska 166, 02-787 Warsaw, Poland, Department of Animal Physiology, Lund University, Helgonavagen 3b, S-223 62 Lund, Sweden, Department of Animal Nutrition and Physiology, Danish Institute of Agricultural Sciences, Research Centre Foulum, PO Box 50, DK-8830 Tjele, Denmark and R. & D. Gramineer Int. AB, Ideon, 223 70 Lund, Sweden
A. Gregard
Affiliation:
Department of Animal Physiology, Faculty of Veterinary Medicine, Warsaw Agricultural University, ul. Nowoursynowska 166, 02-787 Warsaw, Poland, Department of Animal Physiology, Lund University, Helgonavagen 3b, S-223 62 Lund, Sweden, Department of Animal Nutrition and Physiology, Danish Institute of Agricultural Sciences, Research Centre Foulum, PO Box 50, DK-8830 Tjele, Denmark and R. & D. Gramineer Int. AB, Ideon, 223 70 Lund, Sweden
B. Weström
Affiliation:
Department of Animal Physiology, Faculty of Veterinary Medicine, Warsaw Agricultural University, ul. Nowoursynowska 166, 02-787 Warsaw, Poland, Department of Animal Physiology, Lund University, Helgonavagen 3b, S-223 62 Lund, Sweden, Department of Animal Nutrition and Physiology, Danish Institute of Agricultural Sciences, Research Centre Foulum, PO Box 50, DK-8830 Tjele, Denmark and R. & D. Gramineer Int. AB, Ideon, 223 70 Lund, Sweden
M. S. Hedemann
Affiliation:
Department of Animal Physiology, Faculty of Veterinary Medicine, Warsaw Agricultural University, ul. Nowoursynowska 166, 02-787 Warsaw, Poland, Department of Animal Physiology, Lund University, Helgonavagen 3b, S-223 62 Lund, Sweden, Department of Animal Nutrition and Physiology, Danish Institute of Agricultural Sciences, Research Centre Foulum, PO Box 50, DK-8830 Tjele, Denmark and R. & D. Gramineer Int. AB, Ideon, 223 70 Lund, Sweden
H. N. Lærke
Affiliation:
Department of Animal Physiology, Faculty of Veterinary Medicine, Warsaw Agricultural University, ul. Nowoursynowska 166, 02-787 Warsaw, Poland, Department of Animal Physiology, Lund University, Helgonavagen 3b, S-223 62 Lund, Sweden, Department of Animal Nutrition and Physiology, Danish Institute of Agricultural Sciences, Research Centre Foulum, PO Box 50, DK-8830 Tjele, Denmark and R. & D. Gramineer Int. AB, Ideon, 223 70 Lund, Sweden
D. Kruszewska
Affiliation:
Department of Animal Physiology, Faculty of Veterinary Medicine, Warsaw Agricultural University, ul. Nowoursynowska 166, 02-787 Warsaw, Poland, Department of Animal Physiology, Lund University, Helgonavagen 3b, S-223 62 Lund, Sweden, Department of Animal Nutrition and Physiology, Danish Institute of Agricultural Sciences, Research Centre Foulum, PO Box 50, DK-8830 Tjele, Denmark and R. & D. Gramineer Int. AB, Ideon, 223 70 Lund, Sweden
S. G. Pierzynowski
Affiliation:
Department of Animal Physiology, Faculty of Veterinary Medicine, Warsaw Agricultural University, ul. Nowoursynowska 166, 02-787 Warsaw, Poland, Department of Animal Physiology, Lund University, Helgonavagen 3b, S-223 62 Lund, Sweden, Department of Animal Nutrition and Physiology, Danish Institute of Agricultural Sciences, Research Centre Foulum, PO Box 50, DK-8830 Tjele, Denmark and R. & D. Gramineer Int. AB, Ideon, 223 70 Lund, Sweden
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Abstract

The aim of this study was to investigate the role of the parasympathetic (cholinergic and peptidergic) nervous system in the regulation of exocrine pancreas function in piglets during their early postnatal development. The cholinergic and peptidergic regulatory pathways of exocrine pancreatic function were tested by the specific muscarinic receptor blocker 4-diphenylacetoxy-N-methylpiperidine-methiodide (4-DAMP) and bombesin, respectively. At the age of 2 weeks, piglets were surgically fitted with a chronic pancreatic duct catheter, a duodenal re-entrant cannula and a jugular vein catheter. The experiments comprised a pre-weaning period, and a post-weaning period that commenced at the beginning of the 5th week of age. Intravenous infusion of 4-DAMP (100 pmol kg-1 h-1) reduced the outflow of pancreatic juice, the output of total protein and the activity of trypsin, chymotrypsin, carboxyl ester hydrolase and amylase during preprandial and postprandial pancreatic secretion, in both the pre- and post-weaning periods. However, the inhibitory effect of 4-DAMP during postprandial secretion was significantly greater (P < 0.05) in suckling piglets. The infusion of bombesin (10, 100 and 1000 pmol kg-1 h-1) stimulated exocrine pancreatic secretion in a dose-dependent manner during both the pre- and post-weaning periods. However, the stimulatory effect of 1000 pmol kg-1 h-1 bombesin on total protein output and the activities of trypsin, chymotrypsin and amylase were significantly higher (P < 0.05) in suckling piglets. In summary, our study showed that cholinergic and peptidergic mechanisms are involved in the regulation of exocrine pancreas function in piglets in both the pre- and post-weaning stages. 4-DAMP had a greater inhibitory effect on exocrine pancreatic secretion in piglets during the pre-weaning period. Thus, these observations suggest that the parasympathetic nervous system plays a dominant role in the functioning of the exocrine pancreas at this time. The action of bombesin suggests that it is a potent secretagogue for the exocrine pancreas in pigs during their postnatal development. Experimental Physiology (2001) 86.3, 399-409.

Type
Research Article
Information
Experimental Physiology , Volume 86 , Issue 3 , May 2001 , pp. 399 - 409
Copyright
© The Physiological Society 2001

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