Hostname: page-component-76fb5796d-qxdb6 Total loading time: 0 Render date: 2024-04-26T11:14:33.274Z Has data issue: false hasContentIssue false
  • English
  • Français

The effect of carbachol and [alpha]-bungarotoxin on the frequency of miniature endplate potentials at the frog neuromuscular junction

Published online by Cambridge University Press:  25 January 2001

Ela Bukharaeva ,
Tania Ipatova ,
E. E. Nikolsky  and
F. Vyskocil
Ela Bukharaeva
Affiliation:
Kazan Medical University and Kazan Institute of Biophysics and Biochemistry of the Russian Academy of Sciences, Butlerov Str. 49, Kazan, Tatarstan, Russia, and Institute of Physiology, Academy of Sciences of the Czech Republic, Vídenská 1083, 142 20 Prague and Department of Animal Physiology and Developmental Biology , Charles University, Vinicna 7, 128 00 Prague 2, Czech Republic
Tania Ipatova
Affiliation:
Kazan Medical University and Kazan Institute of Biophysics and Biochemistry of the Russian Academy of Sciences, Butlerov Str. 49, Kazan, Tatarstan, Russia, and Institute of Physiology, Academy of Sciences of the Czech Republic, Vídenská 1083, 142 20 Prague and Department of Animal Physiology and Developmental Biology , Charles University, Vinicna 7, 128 00 Prague 2, Czech Republic
E. E. Nikolsky
Affiliation:
Kazan Medical University and Kazan Institute of Biophysics and Biochemistry of the Russian Academy of Sciences, Butlerov Str. 49, Kazan, Tatarstan, Russia, and Institute of Physiology, Academy of Sciences of the Czech Republic, Vídenská 1083, 142 20 Prague and Department of Animal Physiology and Developmental Biology , Charles University, Vinicna 7, 128 00 Prague 2, Czech Republic
F. Vyskocil
Affiliation:
Kazan Medical University and Kazan Institute of Biophysics and Biochemistry of the Russian Academy of Sciences, Butlerov Str. 49, Kazan, Tatarstan, Russia, and Institute of Physiology, Academy of Sciences of the Czech Republic, Vídenská 1083, 142 20 Prague and Department of Animal Physiology and Developmental Biology , Charles University, Vinicna 7, 128 00 Prague 2, Czech Republic
Get access

Abstract

The effects of an acetylcholine analogue, carbachol (CCh), and a purified irreversible nicotinic antagonist, α-bungarotoxin (BTX), on the frequency of the miniature endplate potentials (mEPPs) at the neuromuscular junction of the frog were tested at 20 and 10¡C. CCh (5 ± 10-6 m) reduced the frequency of mEPPs to about 60 %; this reduction was not affected by 1 ± 10-7 g ml-1 BTX. BTX also reversibly decreased the mEPP frequency by 40 %, but not in the presence of CCh or in Ringer solution with 0 or 8 mM Ca2+. The present data show that BTX, which inhibits a class of nicotinic ACh receptors, does not block the decrease of mEPP frequency evoked by CCh and can itself suppress the frequency of spontaneous quantal release.

Type
Research Article
Information
Experimental Physiology , Volume 85 , Issue 2 , March 2000 , pp. 125 - 131
Copyright
© The Physiological Society 2000

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)