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Keratinocyte growth factor and epidermal growth factor can reverse the intestinal atrophy associated with elemental diets in mice

Published online by Cambridge University Press:  12 March 2003

Masaya Sasaki ,
Anthony J. FitzGerald ,
Nikki Mandir ,
Jorge Berlanga-Acosta  and
Robert A. Goodlad
Masaya Sasaki
Affiliation:
Department of Internal Medicine, Shiga University of Medical Science, Seta-tsukinowa, Otsu, Shiga 520-2192, Japan, Department of Histopathology, Imperial College School of Medicine, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK, Histopathology Unit, Cancer Research UK, 44 Lincoln's Inn Fields, London WC2A 3PX, UK and Center for Genetic Engineering and Biotechnology, PO Box 6162, Havana, Cuba
Anthony J. FitzGerald
Affiliation:
Department of Internal Medicine, Shiga University of Medical Science, Seta-tsukinowa, Otsu, Shiga 520-2192, Japan, Department of Histopathology, Imperial College School of Medicine, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK, Histopathology Unit, Cancer Research UK, 44 Lincoln's Inn Fields, London WC2A 3PX, UK and Center for Genetic Engineering and Biotechnology, PO Box 6162, Havana, Cuba
Nikki Mandir
Affiliation:
Department of Internal Medicine, Shiga University of Medical Science, Seta-tsukinowa, Otsu, Shiga 520-2192, Japan, Department of Histopathology, Imperial College School of Medicine, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK, Histopathology Unit, Cancer Research UK, 44 Lincoln's Inn Fields, London WC2A 3PX, UK and Center for Genetic Engineering and Biotechnology, PO Box 6162, Havana, Cuba
Jorge Berlanga-Acosta
Affiliation:
Department of Internal Medicine, Shiga University of Medical Science, Seta-tsukinowa, Otsu, Shiga 520-2192, Japan, Department of Histopathology, Imperial College School of Medicine, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK, Histopathology Unit, Cancer Research UK, 44 Lincoln's Inn Fields, London WC2A 3PX, UK and Center for Genetic Engineering and Biotechnology, PO Box 6162, Havana, Cuba
Robert A. Goodlad
Affiliation:
Department of Internal Medicine, Shiga University of Medical Science, Seta-tsukinowa, Otsu, Shiga 520-2192, Japan, Department of Histopathology, Imperial College School of Medicine, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK, Histopathology Unit, Cancer Research UK, 44 Lincoln's Inn Fields, London WC2A 3PX, UK and Center for Genetic Engineering and Biotechnology, PO Box 6162, Havana, Cuba
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Abstract

Elemental diets are associated with intestinal atrophy and reduced intestinal integrity. Growth factors such as keratinocyte growth factor (KGF) and epidermal growth factor (EGF) have considerable potential for the therapeutic reversal of such atrophy and may have greater actions if given in combination. We examined the effects of recombinant human KGF (rHuKGF), EGF and their combination on tissue mass, cell proliferation and crypt fission throughout the intestine of mice fed elemental diets. rHuKGF significantly increased the relative wet weight of the intestine, with EGF having a lesser effect. Cell proliferation of the stomach, small intestine and colon were significantly increased by rHuKGF, but EGF only increased proliferation in the small intestine. Crypt fission in the small intestine and colon was significantly decreased by rHuKGF. An interactive effect of rHuKGF and EGF on the weight of stomach and the proliferation of the fundus and antrum was observed. Moreover, an interactive effect of the agents was also seen on crypt fission in the colon. We concluded that (1) rHuKGF and EGF have significant trophic effects on the stomach, small intestine and colon, (2) these actions vary between different sites in the gastrointestinal tract, and (3) interactive effects occur. Experimental Physiology (2003) 88.2, 261-267.

Type
Full Length Papers
Information
Experimental Physiology , Volume 88 , Issue 2 , March 2003 , pp. 261 - 267
Copyright
© The Physiological Society 2003

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