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Propofol attenuates ischaemia-reperfusion injury in the rat heart in vivo

Published online by Cambridge University Press:  01 February 2008

I. Kobayashi ,
N. Kokita  and
A. Namiki
I. Kobayashi*
Affiliation:
Asahikawa Red Cross Hospital, Department of Anesthesiology, Asahikawa, Hokkaido, Japan
N. Kokita
Affiliation:
Asahikawa Medical College, Department of Anesthesiology, Asahikawa, Hokkaido, Japan
A. Namiki
Affiliation:
Sapporo Medical University, School of Medicine, Department of Anesthesiology, Hokkaido, Japan
*
Correspondence to: Iwao Kobayashi, Department of Anesthesiology, Asahikawa Red Cross Hospital, Akebono 1-1, Asahikawa 070-8530, Japan. E-mail: iwakoba0116@asahikawa-rch.gr.jp; Tel: +81 166 22 8111; Fax: +81 166 22 5108
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Summary

Background

We have previously demonstrated, in the isolated rat heart, that propofol attenuates hydrogen peroxide-induced damage and ischaemia-reperfusion injury, and that the beneficial effect of propofol is correlated with reduction of the lipid peroxidation. This study was designed to evaluate whether propofol has a cardioprotective effect against ischaemia-reperfusion injury in a rat model in vivo.

Methods

Adult rats were anaesthetized with pentobarbital 10 mg kg−1 h−1 alone (control group), pentobarbital 10 or 20 mg kg−1 h−1 + Intralipid® as a vehicle (Pent-10, Pent-20 group), propofol 10 or 20 mg kg−1 h−1 (Prop-10, Prop-20 group) intravenously throughout the experiment. The left anterior descending coronary artery was occluded for 30 min followed by 120 min of reperfusion. Infarct size was determined at the end of reperfusion. The tissue concentration of malondialdehyde was measured at 30 min after reperfusion to evaluate lipid peroxidation.

Results

The infarct sizes (% of area at risk) were significantly smaller in the Prop-10 (54 ± 11%; P < 0.01 vs. control) and Prop-20 (39 ± 8%; P < 0.01 vs. control) groups than in the control (68 ± 9%), Pent-10 (69 ± 13%) and Pent-20 (68 ± 14%) groups (n = 12). In the Pent-10 and Pent-20 groups, ischaemia-reperfusion produced significant increases in the values for tissue malondialdehyde (0.72 ± 0.24 μmol mg protein−1; P < 0.05 and 0.63 ± 0.33 μmol mg protein−1; P < 0.05 vs. 0.46 ± 0.22 μmol mg protein−1 in non-ischaemic hearts, n = 8). However, the values of malondialdehyde in the Prop-10 and -20 groups were suppressed by 41% and 63%, respectively, compared with the Pent-10 group (P < 0.01).

Conclusion

Our results suggest that propofol could be cardioprotective against ischaemia-reperfusion injury dose dependently in a rat model in vivo and that the beneficial action of propofol may be correlated with its antioxidant effect.

Keywords

ANAESTHETICS INTRAVENOUSPROPOFOLREPERFUSION INJURYLIPID PEROXIDATIONMALONDIALDEHYDEHEARTRAT
Type
Original Article
Information
European Journal of Anaesthesiology , Volume 25 , Issue 2 , February 2008 , pp. 144 - 151
Copyright
Copyright © European Society of Anaesthesiology 2007

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Footnotes

Presented in part at the annual meeting of the American Society of Anesthesiologists, Orlando, FL, USA, October 2002.

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