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The preventive effect of diphenhydramine on bacterial growth in propofol: a laboratory study

Published online by Cambridge University Press:  01 September 2008

A. Güzelant ,
S. Apiliogullari ,
I. Kara ,
V. Turhan ,
B. Apiliogullari ,
H. Yilmaz ,
M. Balasar  and
A. Duman
A. Güzelant
Affiliation:
Meram Teaching & Research Hospital, Department of Microbiology, Konya, Turkey
S. Apiliogullari*
Affiliation:
Ozel Konya Hospital, Department of Anaesthesia and Intensive Care, Konya, Turkey
I. Kara
Affiliation:
Meram Teaching & Research Hospital, Department of Anaesthesia and Intensive Care, Konya, Turkey
V. Turhan
Affiliation:
GATA Haydarpasa Training Hospital, Department of Infectious Diseases and Clinical Microbiology, Konya, Turkey
B. Apiliogullari
Affiliation:
Meram Teaching & Research Hospital, Department of Thoracic Surgery, Konya, Turkey
H. Yilmaz
Affiliation:
Ozel Konya Hospital, Department of General Surgery, Konya, Turkey
M. Balasar
Affiliation:
Ozel Konya Hospital, Department of Urology, Konya, Turkey
A. Duman
Affiliation:
Selcuk University, Medical Faculty, Department of Anaesthesia and Intensive Care, Konya, Turkey
*
Correspondence to: S. Apiliogullari, Department of Anaesthesia and Intensive Care, Ozel Konya Hospital, Konya, Turkey. E-mail: sapiliogullari@yahoo.com; Tel: +90 505 488 70 14; Fax: +90 332 2366066
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Summary

Background and objective

Diphenhydramine has local anaesthetic and antimicrobial activity and may be used to prevent intravenous propofol injection pain. We studied the effect of adding diphenhydramine to propofol emulsions for preventing bacterial growth.

Methods

Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Candida albicans cultures were inoculated into the following solutions: 1% propofol, 0.05% diphenhydramine + 1% propofol, 0.1% diphenhydramine + 1% propofol, 0.2% diphenhydramine + 1% propofol, 0.3% diphenhydramine + 1% propofol, 1% diphenhydramine and 0.1% lidocaine + 1% propofol. A 100-μL of inoculum suspension adjusted for each of the micro-organisms was added separately to each tube and left at 20°C. A 10-μL aliquot of each mixture was inoculated onto blood agar medium at 5 and 24 h. These plates were incubated at 35°C for 24 h. Each plated medium was read, and the number of colony-forming units were counted and recorded (n = 2). Analysis of variance (ANOVA) with a post hoc Tukey HSD test and paired t-tests were used for data analysis. P < 0.05 was considered as significant.

Results

Diphenhydramine inhibited bacterial growth in propofol solutions in a dose-dependent manner. It was more effective than 0.1% lidocaine at similar concentrations in preventing bacterial growth for all organisms.

Conclusion

Diphenhydramine had a significant inhibitory effect on bacterial growth in propofol.

Keywords

DIPHENHYDRAMINELIDOCAINEPROPOFOLINFECTION POSTOPERATIVEDRUG CONTAMINATION, bacterial
Type
Original Article
Information
European Journal of Anaesthesiology , Volume 25 , Issue 9 , September 2008 , pp. 737 - 740
Copyright
Copyright © European Society of Anaesthesiology 2008

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