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Virulence and genotype stability of Salmonella enterica serovar Berta during a natural outbreak

Published online by Cambridge University Press:  15 May 2009

J. E. Olsen ,
M. N. Skov ,
D. J. Brown ,
J. P. Christensen  and
M. Bisgaard
J. E. Olsen*
Affiliation:
Department of Veterinarv Microbiology, The Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark
M. N. Skov
Affiliation:
Department of Veterinarv Microbiology, The Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark
D. J. Brown
Affiliation:
Department of Veterinarv Microbiology, The Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark
J. P. Christensen
Affiliation:
Department of Veterinarv Microbiology, The Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark
M. Bisgaard
Affiliation:
Department of Veterinarv Microbiology, The Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark
*
* Corresponding author and author to whom request for reprints should be addressed.
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Strains of Salmonella enterica serotype Berta, collected over a period of 6 years from a well documented natural outbreak in Denmark, have been characterized in order to assess the stability of chromosomal typing systems and virulence properties. Outbreak strains were identical in Pvu II and Pst I IS200 profiles, all but two strains showed the same Sma I ribotype, and all but one strain showed the same Not I pulsed field gel electrophoretic pattern, indicating that these molecular markers remained almost constant during the outbreak. In general, strains of S. Berta were found to be of moderate to low virulence; log VC10 values were found to vary between 3·0 and 4·4 after i.p. challenge of mice, and maximum CFU in internal organs of day-old chicks varied between 2 and 4 log10 units following oral challenge. The minor differences observed between strains in vivo did not correlate with differences in in vitro invasion into cultured MDCK cells, nor with in vitro growth characteristics. A succession of different plasmid profile types was observed during the outbreak but a hierarchical selection of clones based on differences in virulence was unlikely to have caused the succession of types of S. Berta during this outbreak.

Type
Special Article
Information
Epidemiology & Infection , Volume 116 , Issue 3 , June 1996 , pp. 267 - 274
Copyright
Copyright © Cambridge University Press 1996

References

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