Hostname: page-component-848d4c4894-mwx4w Total loading time: 0 Render date: 2024-06-25T12:48:37.343Z Has data issue: false hasContentIssue false
  • English
  • Français

A survey on the occurrence of Vibrio parahaemolyticus on fish and shellfish, marketed in The Netherlands

Published online by Cambridge University Press:  15 May 2009

E. H. Kampelmacher ,
D. A. A. Mossel ,
L. M. van Noorle Jansen  and
H. Vincentie
E. H. Kampelmacher
Affiliation:
National Institute of Public Health, Utrecht, The Netherlands
D. A. A. Mossel
Affiliation:
National Institute of Public Health, Utrecht, The Netherlands
L. M. van Noorle Jansen
Affiliation:
Central Institute for Nutrition & Food Research T.N.O., Zeist, The Netherlands
H. Vincentie
Affiliation:
Central Institute for Nutrition & Food Research T.N.O., Zeist, The Netherlands
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A survey was carried out on the occurrence of Vibrio parahaemolyticus on fish and shellfish, as sold in The Netherlands.

The optimal mode of detection of this bacterium appeared to be: (i) enrichment of swabs taken from the surface and the gills in freshly prepared meat broth with 5% NaCl; (ii) streaking onto Teepol bromothymol blue agar (BTB) and taurocholate bromothymol blue sucrose agar (TCBS); (iii) confirmation of suspect colonies by testing for mode of growth in butts/slants of a Kligler type glucose sucrose iron thiosulphate agar, formation of indole in 2% NaCl 2% trypticase water, anaerobic utilization of starch in the presence of 5% NaCl and oxidase reaction according to Kovacs (1956).

A total of 407 samples, stemming from 17 types of fish and shellfish, taken at three fish shops, was examined by this technique. Only one specimen, i.e. a haddock, was found to contain V. parahaemolyticus. This contamination rate of approximately 0·3% correlates well with data found earlier for fish landed in Northern Germany.

Type
Research Article
Information
Journal of Hygiene , Volume 68 , Issue 2 , June 1970 , pp. 189 - 196
Copyright
Copyright © Cambridge University Press 1970

References

REFERENCES

Akiyama, S., Takizawa, K., Ichinohe, M., Enomoto, S., Kobayashi, S. & Sakazaki, R. (1963) Application of teepol to the isolation medium for the enteropathogenic halophilic vibrios. Japanese Journal of Bacteriology 18, 255.Google Scholar
Baross, J. & Liston, J. (1968). Isolation of Vibrio parahaemolyticus from the Northwest Pacific. Nature London 217, 1263–4.CrossRefGoogle ScholarPubMed
Greenberg, R. A. & Silliker, J. H. (1962). The effect of tylosin on coagulase positive staphylococci in food products. Journal of Food Science 27, 60–3.CrossRefGoogle Scholar
Greenberg, R. A. & Silliker, J. H. (1962). The action of tylosin on sporeforming bacteria. Journal of Food Science 27, 64–8.CrossRefGoogle Scholar
Kobayashi, T., Enomoto, S., Sakazaki, R. & Kuwabara, S. (1963). A new selective isolation medium for pathogenic vibrios: TCBS-agar. Japanese Journal of Bacteriology 18, 387–91.Google Scholar
Kovacs, N. (1956). Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature London 178, 703.Google Scholar
Krantz, G. E., Colwell, R. R. & Lovelace, E. (1969). Vibrio parahaemolyticus from the blue crab Callinectes sapidus in Chesapeake bay. Science 164, 1286–7.Google Scholar
Mossel, D. A. A. (1959). Enumeration of sulphite-reducing clostridia occurring in foods. Journal of the Science of Food & Agriculture 10, 662–9.Google Scholar
Mossel, D. A. A. (1969). Microbiological quality control in the food industry. Journal of Milk & Food Technology 32, 155–71.CrossRefGoogle Scholar
Mossel, D. A. A., Kampelmacher, E. H. & Van Noorle Jansen, L. M. (1968). The optimal mode of transport for swabs obtained from surfaces examined for organisms causing food-borne disease. Journal of Hygiene 66, 59–66.CrossRefGoogle ScholarPubMed
Mossel, D. A. A. & Martin, G. (1961). Milieu simplifié permettant l'étude des divers modes d'action des bactéries sur les hydrates de carbone. Annales de l' Institut Pasteur de Lille 12, 225–6.Google Scholar
Nakanishi, H., Leistner, L. & Baumgart, J. (1967). Nachweis von Vibrio alginolyticus bei Seefischen in Deutschland. Archiv für Lebensmittelhygiene 18, 201–2.Google Scholar
Nakanishi, H., Leistner, L., Hechelmann, H. & Baumgart, J. (1968). Weitere Untersuchungen über das Vorkommen von Vibrio parahaemolyticus und Vibrio alginolyticus bei Seefischen in Deutschland, Archiv. für Lebensmittelhygiene 19, 49–53.Google Scholar
Sakazaki, R. (1965). Vibrio parahaemolyticus. A non-choleragenic enteropathogenic Vibrio. Proceedings of the Cholera Research Symposium. Honolulu, Hawaii, 30–34 (Dept. of Health, Education and Welfare, Public Health Service, Washington).Google Scholar
Sakazaki, R., Iwanami, S. & Fukumi, H. (1963). Studies on the enteropathogenic, facultatively halophilic bacteria, Vibrio parahaemolyticus. I. Morphological, cultural and biochemical properties and its taxonomic position. Japanese Journal of Medical Science & Biology 16, 161–88.CrossRefGoogle Scholar
Sakazaki, R., Tamura, K., Kato, T., Obara, Y., Yamai, S. & Hobo, K. (1968). Studies on the enteropathogenic, facultatively halophilic bacteria, Vibrio parahaemolyticus. III. Entero-pathogenicity. Japanese Journal of Medical Science & Biology 21, 325–31.CrossRefGoogle Scholar
Schubert, R. H. W. (1967). Das Vorkommen der Aeromonaden in oberirdischen Gewässern. Archiv für Hygiene und Bakteriologie (Berl.) 150, 688–708.Google Scholar
Temmyo, R. (1966). Studies on the prevention of outbreaks of food poisoning caused by Vibrio parahaemolyticus. Bulletin of Tokyo Medical & Dental University 13, 489–510.Google ScholarPubMed
Ward, B. Q. (1968). Isolations of organisms related to Vibrio parahaemolyticus from American estuarine sediments. Applied Microbiology 16, 543–6.Google Scholar
Zen-Yoji, H., Sakai, S., Terayama, T., Kudo, Y., Ito, T., Benoki, M. & Nagasaki, M. (1965). Epidemiology, enteropathogenicity and classification of Vibrio parahaemolyticus. Journal of Infectious Diseases 115, 436–44.CrossRefGoogle Scholar