Hostname: page-component-77c89778f8-m8s7h Total loading time: 0 Render date: 2024-07-18T03:01:52.273Z Has data issue: false hasContentIssue false

Survey on the distribution of the gene 4 alleles of human rotaviruses by polymerase chain reaction

Published online by Cambridge University Press:  15 May 2009

H. Wu
Affiliation:
Department of Hygiene, School of Medicine, Sapporo Medical University. Sapporo 060, Japan
K. Taniguchi
Affiliation:
Department of Hygiene, School of Medicine, Sapporo Medical University. Sapporo 060, Japan
F. Wakasugi
Affiliation:
Department of Hygiene, School of Medicine, Sapporo Medical University. Sapporo 060, Japan
S. Ukae
Affiliation:
Department of Pediatrics, School of Medicine, Sapporo Medical University. Sapporo 060, Japan
S. Chiba
Affiliation:
Department of Pediatrics, School of Medicine, Sapporo Medical University. Sapporo 060, Japan
M. Ohseto
Affiliation:
Ehime Institute of Public Health, Matsuyama, Japan
A. Hasegawa
Affiliation:
Department of Epidemiology, National Institute of Health, Tokyo, Japan
Tomoko Urasawa
Affiliation:
Department of Hygiene, School of Medicine, Sapporo Medical University. Sapporo 060, Japan
Shozo Urasawa
Affiliation:
Department of Hygiene, School of Medicine, Sapporo Medical University. Sapporo 060, Japan
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The presence of six gene 4 alleles (or VP4 genotypes) in human rotaviruses has been recognized. Using 16 representative cultivable human rotavirus strains, we confirmed the specificity of VP4 genotyping by polymerase chain reaction (PCR) using the nested oligonucleotides specific to each of the four representative gene 4 alleles. Using the PCR. we surveyed the gene 4 alleles of 199 human rotaviruses in stools collected in Japan and Thailand. Strains with the gene 4 allele, corresponding to P1A serotype. were shown to be the most prevalent, but two strains with P2 gene 4 allele and one strain with P3 gene 4 allele were detected in Thailand and in Japan, respectively.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1994

References

REFERENCES

1.Kapikian, AZ, Chanock, RM. Rotaviruses. In: Fields, BN, Knipe, DM, Chanock, RM et al. , eds. Virology. New York: Raven Press. 1990: 1353–404.Google Scholar
2.Estes, M, Cohen, J. Rotavirus gene structure and function. Microbiol Rev 1989; 53: 410–49.CrossRefGoogle ScholarPubMed
3.Kapikian, AZ, Flores, J, Hoshino, Y et al. , Rotavirus: the major etiologic agent of severe infantile diarrhea may be controllable by a ‘Jennerian’ approach to vaccination. J Infect Dis 1986; 153: 815–22.CrossRefGoogle ScholarPubMed
4.Hoshino, Y, Sereno, MM, Midthun, K, Flores, J, Kapikian, AZ, Chanock, RM. Independent segregation of two antigenic specificities (VP3 and VP7) involved in neutralization of rotavirus infectivity. Proc Natl Acad Sci USA 1985; 832: 8701–4.CrossRefGoogle Scholar
5.Urasawa, S, Urasawa, T, Wakasugi, F et al. , Presumptive seventh serotype of human rotavirus. Arch Virol 1990; 113: 279–82.CrossRefGoogle ScholarPubMed
6.Beards, G, Xu, L, Ballard, A, Desselberger, U, McCrae, MA. A serotype 10 human rotavirus. J Clin Microbiol 1992; 30: 1432–5.CrossRefGoogle ScholarPubMed
7.Urasawa, S, Hasegawa, A, Urasawa, T et al. Antigenic and genetic analysis of human rotaviruses prevailing in Chiang Mai, Thailand: evidence for a close relationship between human and animal rotaviruses. J Infect Dis 1992; 166: 227–34.CrossRefGoogle Scholar
8.Das, M, Dunn, SJ, Woode, GN, Greenberg, HB, Rao, D. Both surface proteins (VP4 and VP7) of an asymptomatic neonatal rotavirus strain (I321) have high levels of sequence identity with the homologous proteins of a serotype 10 bovine rotavirus. Virology 1993; 194: 374–9.CrossRefGoogle ScholarPubMed
9.Taniguchi, K, Urasawa, T, Morita, Y, Greenberg, HB, Urasawa, S. Direct serotyping of human rotavirus in stools by an enzyme-linked immunosorbent assay using serotype 1-, 2-, 3-, and 4-specific monoclonal antibodies to VP7. J Infect Dis 1987; 155: 1159–66.CrossRefGoogle ScholarPubMed
10.Urasawa, S, Urasawa, T, Taniguchi, K et al. , Survey of human rotavirus serotypes in different locales in Japan by enzyme-linked immunosorbent assay with monoclonal antibodies. J Infect Dis 1989: 160: 4451.CrossRefGoogle ScholarPubMed
11.Woods, PA, Gentsch, J, Gouvea, V et al. , Distribution of serotypes of human rotavirus in different populations. J Clin Microbiol 1992; 30: 781–5.CrossRefGoogle ScholarPubMed
12.Gorziglia, M, Larralde, G, Kapikian, AZ, Chanock, RM. Antigenic relationships among human rotaviruses as determined by outer capsid protein VP4. Proc Natl Acad Sci USA 1990; 87: 7155–9.CrossRefGoogle ScholarPubMed
13.Li, B, Larralde, G, Gorziglia, M. Human rotavirus K8 strain represents a new VP4 serotype. J Virol 1993; 67: 617–20.CrossRefGoogle ScholarPubMed
14.Matsui, SM, Offit, PA, Mackow, ER, Benfield, DA, Shaw, RD, Greenberg, HB. Passive protection against rotavirus-induced diarrhea by monoclonal antibodies to the heterotypic neutralization domain of VP7 and VP8 fragment of VP4. J Clin Microbiol 1989; 27: 780–2.CrossRefGoogle Scholar
15.Gorziglia, M, Green, K, Nishikawa, K et al. , Sequence of the fourth gene of human rotaviruses recovered from asymptomatic or symptomatic infections. J Virol 1988; 62: 2978–84.CrossRefGoogle ScholarPubMed
16.Taniguchi, K, Nishikawa, K, Urasawa, T et al. , Complete nucleotide sequence of the gene encoding VP4 of a human rotavirus (strain K8) which has unique VP4 neutralization epitopes. J Virol 1989; 63: 4101–6.CrossRefGoogle ScholarPubMed
17.Gentsch, JR, Glass, RI, Woods, P et al. , Identification of group A rotavirus gene 4 types by polymerase chain reaction. J Clin Microbiol 1992; 30: 1365–73.CrossRefGoogle Scholar
18.Taniguchi, K, Urasawa, T, Kobayashi, N, Gorziglia, M, Urasawa, S. Nucleotide sequence of VP4 and VP7 genes of human rotaviruses with subgroup I specificity and long RNA pattern: implication for new G serotype specificity. J Virol 1990; 64: 5640–4.CrossRefGoogle ScholarPubMed
19.Urasawa, T, Taniguchi, K, Kobayashi, N et al. , Antigenic and genetic analyses of human rotavirus with dual subgroup specificity. J Clin Microbiol 1990; 28: 2837–41.CrossRefGoogle ScholarPubMed
20.Isegawa, Y, Nakagomi, O, Nakagomi, T, Ueda, S. A VP4 sequence highly conserved in human rotavirus strain AU-1 and feline rotavirus strain FRV-1. J Gen Virol 1992; 73: 1939–46.CrossRefGoogle ScholarPubMed
21.Taniguchi, K, Wakasugi, F, Pongsuwanna, Y et al. Identification of human and bovine rotavirus serotypes by polymerase chain reaction. Epidemiol Infect 1992; 28: 276–82.Google Scholar
22.Gouvea, V, Glass, RI, Woods, P et al. , Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens. J Clin Microbiol 1990; 28: 276–82.CrossRefGoogle ScholarPubMed
23.Mackow, ER, Shaw, RD, Matsui, SM, Vo, PT, Dang, MN, Greenberg, HB. Characterization of the rhesus rotavirus VP3 gene: location of amino acids involved in homologous and heterologous rotavirus neutralization and identification of a putative fusion region. Proc Natl Acad Sci USA 1988; 85: 645–9.CrossRefGoogle Scholar
24.Taniguchi, K, Maloy, WL, Nishikawa, K et al. , Identification of cross-reactive and serotype-2 specific neutralization epitopes on VP3 of human rotavirus. J Virol 1988; 62: 2421–6.CrossRefGoogle ScholarPubMed
25.Larralde, G, Li, BG, Kapikian, AZ, Gorziglia, M. Serotype-specific epitope(s) present on the VP8 subunit of rotavirus VP4 protein. J Virol 1991; 65: 3213–18.CrossRefGoogle ScholarPubMed
26.Steele, DA, Garcia, D, Sears, J, Gerna, G, Nakagomi, O, Flores, J. Distribution of VP4 gene alleles in human rotaviruses by using probes to the hyperdivergent region of the VP4 gene. J Clin Microbiol 1993; 31: 1735–40.CrossRefGoogle Scholar
27.Qian, Y, Green, KY. Human rotavirus strain 69M has a unique VP4 as determined by amino acid sequence analysis. Virology 1991; 182: 407–12.CrossRefGoogle Scholar
28.Taniguchi, K, Urasawa, T, Urasawa, S. Independent segregation of the VP4 and the VP7 genes in bovine rotaviruses as confirmed by VP4 sequence analysis of G8 and G10 bovine rotavirus strains. J Gen Virol 1993; 74: 1215–21.CrossRefGoogle ScholarPubMed
29.Gentsch, JR, Das, BK, Jiang, B, Bhan, MK, Glass, RI. Similarity of the VP4 protein of human rotavirus strain 116E to that of the bovine B223 strain. Virology 1993; 194: 424–30.CrossRefGoogle ScholarPubMed
30.Li, B, Clark, HR, Gouvea, V. Nucleotide sequence of the VP4-encoding gene of an unusual human rotavirus (HCR3). Virology. 1993; 196: 825–30.CrossRefGoogle ScholarPubMed
31.Taniguchi, K, Urasawa, T, Urasawa, S. Species specificity and interspecies relatedness in VP4 genotypes demonstrated by VP4 sequence analysis of equine, feline and canine rotavirus strains. Virology. In press.Google Scholar
32.Sereno, M, Gorziglia, M. The outer capsid protein VP4 of murine rotavirus Eb represents a tentative new P type. Virology. In press.Google Scholar