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Studies on the effect of growth medium composition on the antigenicity of Mycoplasma bovis

Published online by Cambridge University Press:  15 May 2009

C. J. Thorns  and
E. Boughton
C. J. Thorns
Affiliation:
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, New Haw, Weybridge, Surrey
E. Boughton
Affiliation:
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, New Haw, Weybridge, Surrey
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Serum proteins adsorbed from the culture medium were detected in Mycoplasma bovis antigens, the number and type of proteins depending on the serum used in the medium. The α-globulins cross-reacted with α-globulins from different types of sera but the γ-globulins did not. The removal of non-specific medium antibodies by absorption showed that they affected the gel diffusion and growth precipitation tests, producing cross-reactions between M. bovis and M. bovigenitalium, but that the complement fixation, tube agglutination, and growth inhibition tests were not similarly affected. The presence of serum proteins in the antigens changed their specific reactivity in all the tests. The production of antibodies to serum proteins was increased by the use of an adjuvant, but it appeared that production of specific antibodies to the mycoplasmas was not.

Type
Research Article
Information
Journal of Hygiene , Volume 84 , Issue 1 , February 1980 , pp. 29 - 36
Copyright
Copyright © Cambridge University Press 1980

References

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