Hostname: page-component-7479d7b7d-rvbq7 Total loading time: 0 Render date: 2024-07-13T16:08:19.788Z Has data issue: false hasContentIssue false
  • English
  • Français

A standard technique for the isolation of Salmonella from animal feeds

Published online by Cambridge University Press:  15 May 2009

P. J. Smith
P. J. Smith
Affiliation:
Veterinary Investigation Centre, Crown Street, Liverpool L7 7EH
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Two trials were carried out by 9 and 47 laboratories to evaluate the reproducibility and sensitivity of a standard technique for the isolation of salmonellas from animal feeding stuffs.

The trials involved the examinations of test samples containing known numbers of salmonellas by a buffered peptone water pre-enrichment technique. Statistical analysis of the results of the trials showed that the method was capable of detecting salmonellas in most probable numbers as low as 2·3 per 100 g and that no significant differences in performance were observed between laboratories.

The standard technique described is recommended for the examination of animal feed and conforms to international recommendations.

Type
Research Article
Information
Journal of Hygiene , Volume 79 , Issue 3 , December 1977 , pp. 449 - 461
Copyright
Copyright © Cambridge University Press 1977

References

REFERENCES

Carlson, Virginia L. & Snoeyenbos, G. H. (1974). Comparative efficiences of selenite and tetrathionate broths for the isolation of salmonella serotypes. American Journal of Veterinary Research 35, 711–18.Google Scholar
Edel, W. & Kampelmacher, E. H. (1969). Salmonella isolation in nine European laboratories, using a standard technique. Bulletin of the World Health Organization 41, 297–306.Google Scholar
Edel, W. & Kampelmacher, E. H. (1973). Comparative studies on the isolation of ‘sublethally injured’ salmonellae in nine European laboratories. Bulletin of the World Health Organization 48, 167–74.Google ScholarPubMed
Edel, W. & Kampelmacher, E. H. (1974). Comparative studies on salmonella isolations from feeds in ten laboratories. Bulletin of the World Health Organization 50, 421–6.Google ScholarPubMed
McCoy, J. H. (1962). The isolation of Salmonella. Journal of Applied Bacteriology 25, 213–24.CrossRefGoogle Scholar
Magee, M. F. & Hinton, M. (1974). The growth of Salmonella dublin and Salmonella typhimurium on MacConkey media containing brilliant green. Medical Laboratory Technology 31, 179–83.Google ScholarPubMed
Rolfe, V. (1946). A note on the preparation of tetrathionate broth. Monthly Bulletin of the Ministry of Health and the Emergency Public Health Laboratory Service 5, 158.Google ScholarPubMed
Sharma, R. M. & Packer, R. A. (1969). Evaluation of culture media for the isolation of salmonellae from feces. Applied Microbiology 18, 589–95.CrossRefGoogle ScholarPubMed