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The specificity of the anti-haemagglutinin antibody response induced in man by inactivated influenza vaccines and by natural infection

Published online by Cambridge University Press:  15 May 2009

J. S. Oxford ,
G. C. Schild ,
C. W. Potter  and
R. Jennings
J. S. Oxford
Affiliation:
Division of Virology, National Institute for Biological Standards and Control, Holly Hill, Hampstead, London NW3 6RB
G. C. Schild
Affiliation:
Division of Virology, National Institute for Biological Standards and Control, Holly Hill, Hampstead, London NW3 6RB
C. W. Potter
Affiliation:
Department of Virology, The Medical School, Hallamshire Hospital, University of Sheffield, Sheffield S10
R. Jennings
Affiliation:
Department of Virology, The Medical School, Hallamshire Hospital, University of Sheffield, Sheffield S10
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Summary

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The anti-haemagglutinin antibody response in adult human volunteers to inactivated whole virus or tween ether split influenza A/Victoria/75 (H3N2) and A/Scotland/74 (H3N2) virus vaccines was investigated using antibody adsorption and single-radial-haemolysis (SRH) techniques. The concentrations of haemagglutinin (HA), nucleoprotein (NP) and matrix (M) antigens measured by single radial diffusion (SRD) and rocket immunoelectrophoresis were similar for both the whole virus and split vaccines. Whole virus and split vaccines induced cross-reactive (CR) antibody in 87% of vaccinees. Strain specific (SS) antibody to A/Hong Kong/1/68 or the homologous virus was induced less frequently than CR antibody. Higher anti-haemagglutinin antibody titres were detected in persons receiving the split virus vaccines than in those receiving the whole virus vaccines. No antibody to the type-specific matrix protein was detectable, but 33% of volunteers developed an antibody rise to type-specific nucleoprotein antigen.

The specificity of the anti-haemagglutinin antibody response in human adults to natural infection with A/Port Chalmers/73 (H3N2) virus was similar to that induced by inactivated vaccines in that a high proportion of subjects developed CR anti-haemagglutinin antibody, which reacted with A/Hong Kong/68 virus and the homologous A/Port Chalmers/73 virus, and SS antibody for A/Hong Kong/68 virus but SS antibody for A/Port Chalmers/73 virus was infrequently stimulated by natural infection.

Type
Research Article
Information
Journal of Hygiene , Volume 82 , Issue 1 , February 1979 , pp. 51 - 61
Copyright
Copyright © Cambridge University Press 1979

References

REFERENCES

Francis, T. (JR)., Davenport, F. M. & Hennessey, A. V. (1953). Serological recapitulation of human infection with different strains of influenza virus. Transactions of the Association of American Physicians 66, 231.Google Scholar
Jennings, R., Clark, A., Oxford, J. S. & Potter, C. W. (1978). The reactogenicity and immunogenicity of whole and ether-tween split influenza virus vaccines in volunteers. Journal of Infectious Diseases (in Press.)CrossRefGoogle ScholarPubMed
Larson, H. E., Tyrrell, D. A. J., Bowker, C. H., Potter, C. W. & Schild, G. C. (1978). Immunity to challenge in volunteers vaccinated with an inactivated current or earlier strain of influenza A (H3N2). Journal of Hygiene 80, 243–8.CrossRefGoogle ScholarPubMed
Laver, W. G. & Valentine, R. C. (1969). Morphology of the isolated haemagglutinin and neuraminidase subunits of influenza virus. Virology 38, 105–19.Google Scholar
Laver, W. G., Downie, J. C. & Webster, R. G. (1974). Studies on antigenic variation in influenza virus. Evidence for multiple antigenic determinants on the haemagglutinin subunits of A/Hong Kong/68 (H3N2) virus and the A/England/72 strains. Virology, 59, 230–44.CrossRefGoogle Scholar
Mostow, S. R., Schild, G. C., Dowdle, W. R. & Wood, R. J. (1975). Application of the single radial diffusion test for assay of antibody to influenza type A viruses. Journal of Clinical microbiology 2, 531–40.CrossRefGoogle ScholarPubMed
Nermut, M. V. (1972). Further investigation on the fine structure of influenza virus. Journal of General Virology 17, 317–31.CrossRefGoogle ScholarPubMed
Oxford, J. S. & Schild, G. C. (1976). Immunological and physicochemical studies of influenza Matrix (M) polypeptides. Virology 74, 394–402.CrossRefGoogle ScholarPubMed
Oxford, J. S. & Schild, G. C. (1977). Quantification of influenza virus structural proteins using rocket immunoelectrophoresis. Journal of General Virology 38, 187–93.CrossRefGoogle Scholar
Potter, C. W., Jennings, R., Nicholson, K., Tyrrell, D. A. J. & Dickinson, K. G. (1977). Immunity to attenuated influenza virus WRL 105 infection induced by heterologous inactivated influenza A virus vaccines. Journal of Hygiene 79, 321–32.CrossRefGoogle ScholarPubMed
Schild, G. C., Oxford, J. S., Dowdle, W. R., Coleman, M. T., Pereira, M. S. & Chakraverty, P. (1974). Antigenic variation in current influenza A viruses: Evidence for a high frequency of antigenic drift in the Hong Kong virus. Bulletin of the World Health Organization 51, 1–11.Google ScholarPubMed
Schild, G. C., Oxford, J. S. & Virelizier, J. L. (1976). The immune response to influenza – methods of analysis for antibodies and antigens. In The Role of Immunological Factors in Infectious, Allergic and Autoimmune Processes (ed. Beers, R. F. and Bassett, E. G.). New York: Raven Press.Google Scholar
Schild, G. C., Smith, J. W. G., Cretescu, L., Newman, R. W. & Wood, J. M. (1977). Strain specificity of antibody to haemagglutinin following inactivated A/PC/73 vaccine in man: evidence for a paradoxical strain specific antibody response. Developments in Biological Standardisation 39, 273–81.Google Scholar
Schulze, I. T. (1972). The structure of influenza virus. II. A model based on the morphology and composition of subviral particles. Virology 47, 181–96.CrossRefGoogle Scholar
Selby, P. (1976). (ed.). Influenza, Virus, Vaccines and Strategy. London: Academic Press.Google Scholar
Skehel, J. J. & Schild, G. C. (1971). The polypeptide composition of influenza A viruses. Virology 44, 396–408.CrossRefGoogle ScholarPubMed
Vaananen, P., Hovi, T., Helle, E. P. & Penttinen, K. (1976). Determination of mumps and influenza antibodies by haemolysis-in-gel. Archives of Virology 52, 91–9.Google Scholar
Virelizier, J. L., Allison, A. C. & Schild, G. C. (1974b). Antibody responses to antigenic determinants of influenza virus haemagglutinin. II. Original antigenic sin: a bone-marrow derived lymphocyte memory phenomenon modulated by thymus-derived lymphocytes. Journal of Experimental Medicine 140, 1571–8.Google Scholar
Virelizier, J. L., Postlethwaite, R., Schild, G. C. & Allison, A. C. (1974a). Antibody responses to antigenic determinants of influenza virus haemagglutinin. I. Thymus dependence of antibody formation and thymus independence of immunological memory. Journal of Experimental Medicine 140, 1559–70.Google Scholar
Virelizier, J. L. (1975). Host defences against influenza viruses: The role of anti-haemagglutinin antibody. Journal of Immunology 115, 434–9.CrossRefGoogle Scholar
Webster, R. G., Kasel, J. A., Couch, R. B. & Laver, W. G. (1976). Influenza virus subunit vaccines. II. Immunogenicity and original antigenic sin in humans. Journal of Infectious Diseases 134, 48–58.CrossRefGoogle ScholarPubMed
Wood, J. M., Schild, G. C., Newman, R. W. & Seagroatt, V. (1977). Application of an improved single radial immunodiffusion technique for the assay of haemagglutinin antigen content of whole virus and subunit influenza vaccines. Developments in Biological Standardisation 39, 193–200.Google ScholarPubMed