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A solid-phase enzyme linked immunosorbent assay for the detection of African swine fever virus antigen and antibody

Published online by Cambridge University Press:  15 May 2009

R. C. Wardley ,
E. M. E. Abu Elzein ,
J. R. Crowther  and
P. J. Wilkinson
R. C. Wardley
Affiliation:
The Animal Virus Research Institute, Pirbright, Surrey GU24 0NF, England
E. M. E. Abu Elzein
Affiliation:
The Animal Virus Research Institute, Pirbright, Surrey GU24 0NF, England
J. R. Crowther
Affiliation:
The Animal Virus Research Institute, Pirbright, Surrey GU24 0NF, England
P. J. Wilkinson
Affiliation:
The Animal Virus Research Institute, Pirbright, Surrey GU24 0NF, England
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Summary

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A solid-phase enzyme-linked immunosorbent assay was developed to measure both African swine fever virus (ASFV) antigen and antibody. Experiments showed it to be reproducible and able to detect limiting antigen concentrations of 50–500 HAD50/ml. The assay was more sensitive than those used at present to detect ASFV antibody and it is suggested that it might be of great diagnostic use in countries where African swine fever has recently appeared.

Type
Research Article
Information
Journal of Hygiene , Volume 83 , Issue 2 , October 1979 , pp. 363 - 370
Copyright
Copyright © Cambridge University Press 1979

References

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