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Seroepidemiology of group B streptococcus type II antibody specificity

Published online by Cambridge University Press:  15 May 2009

B. M. Gray ,
D. G. Pritchard ,
J. D. Springfield ,
R. J. Benak  and
H. C. Dillon Jr
B. M. Gray*
Affiliation:
Departments of Pediatrics and Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
D. G. Pritchard*
Affiliation:
Departments of Pediatrics and Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
J. D. Springfield*
Affiliation:
Departments of Pediatrics and Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
R. J. Benak*
Affiliation:
Departments of Pediatrics and Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
H. C. Dillon Jr*
Affiliation:
Departments of Pediatrics and Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
*
Correspondence to B. M. Gray, M.D.University of Albama at Birmingham, Department of pediatrics, University station, Birmingham, Alabama, 35294
Correspondence to B. M. Gray, M.D.University of Albama at Birmingham, Department of pediatrics, University station, Birmingham, Alabama, 35294
Correspondence to B. M. Gray, M.D.University of Albama at Birmingham, Department of pediatrics, University station, Birmingham, Alabama, 35294
Correspondence to B. M. Gray, M.D.University of Albama at Birmingham, Department of pediatrics, University station, Birmingham, Alabama, 35294
Correspondence to B. M. Gray, M.D.University of Albama at Birmingham, Department of pediatrics, University station, Birmingham, Alabama, 35294
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The specificity of human antibodies for the two major sidechain determinants of the type II group B streptococcal (GBS) polysaccharide was examined in 90 pairs of maternal and cord sera. Using an ELISA system, total antibody was measured against the complete (sialylated) type II antigen and the proportion of antibody against the galactose determinant was estimated by inhibition with free β–methylgalactopyranosided. Mothers colonized by type II or by other GBS types had higher levels of total specific antibody (means, 33 and 4–7 µg/ml, respectively) than those not colonized (mean, 2–2 µg/ml). Cord sera averaged 1–2 µg/ml lower than maternal sera. Colonization with GBS was also associated with higher levels against the galactose determinant (mean, 1.5&µg/ml, compared to 0–7µg/ml for those not colonized). The distribution of specificities favoured antibodies against the sialic acid determinant in maternal but not cord sera. Specificity as well as antibody level may play a role in the epidemiology of GBS type II.

Type
Research Article
Information
Epidemiology & Infection , Volume 101 , Issue 2 , October 1988 , pp. 347 - 354
Copyright
Copyright © Cambridge University Press 1988

References

Anthony, B. F., Concepcion, N. F., Wass, C. A. & Heiner, D. C. (1984). Immunoglobulin G and M composition of naturally occurring antibody to type III group B streptococci. Infection and Immunity 46, 98–104.CrossRefGoogle Scholar
De Cueninck, B. J., Shockman, G. D. & Swenson, R. M. (1982). Group B, type III streptococcal cell wall: composition and structural aspects revealed through endo–N–acetylmuramidase–catalyzed hydrolysis. Infection and Immunity 35, 572–82.CrossRefGoogle Scholar
Dillon, H. C., Jr., Gray E., Pass, M. A. & Gray, B. M. (1982). Annorectal and vaginal carriage of group B streptococci during pregnancy. Journal of Infectious Disease 145, 794–99.CrossRefGoogle Scholar
Dillon, H. C., Jr., Khare S. & Gray, B. M. (1987). GBS carriage and disease: a six–year prospective study. Journal of Pediatrics 110, 31–36.CrossRefGoogle Scholar
Egan, M. L., Pritchard, D. G., Dillon, H. C. & Gray, B. M. (1983). Protection of mice from experimental infection with type III group B streptococcus using monoclonal antibodies. Journal of Experimental Medicine 158, 1106–1011.CrossRefGoogle ScholarPubMed
Freimer, E. H. (1967). Type–specific polysaccharide antigens of group B streptococci. II. The chemical basis for serological specificity of the type II HCL antigen. Journal of Experimental Medicine 125, 381–92.CrossRefGoogle Scholar
Gray, B. M. (1979). ELISA methodology for polusaccharide antigens: protein coupling of polysaccharides for adsorption to plastic tubes. Journal of Immunological Methods 28, 187–92.CrossRefGoogle ScholarPubMed
Gray, B. M. & DillonH. C., Jr. H. C., Jr. (1985). GBS infections in mothers and their infants. Antimicrobial Chemotherapy 35, 225–36.Google ScholarPubMed
Gray, B. M., Dillon, H. C., Pritchard, D. G., Feinstein, R., Oh, K. & Michalek, S. M. (1987). Type–specific antibodies against group B streptococci in human serum, saliva and vaginal secretions. In Recent Advances in Mucosal Immunology, Part B (ed. Mestecky, J., McGhee, J. R., Bienenstock, J. and Ogra, P. L.), pp. 995–1003, New York: Plenum.Google Scholar
Gray, B. M., Egan, M. L. & Pritchard, D. G. (1988). Specificity of monocolonal antibodies against group B Streptococcus type II and inhibition of their binding by human secretions. Pediatric Research 24, 68–72.CrossRefGoogle Scholar
Gray, B. M., Pass, M. A. & DillonH. C., Jr. H. C., Jr. (1979). Laboratory and field evaluation of selective media for isolation of group B streptococci. Journal of Clinical Microbiology 9, 466–470.CrossRefGoogle ScholarPubMed
Gray, B. M., Pritchard, D. G. & DillonH. C., Jr. H. C., Jr. (1985). Seroepidemiological studies of group B Streptococcus type II. Journal of Infectious Disease 151, 1073–80.CrossRefGoogle ScholarPubMed
Gray, B. M., Pritchard, D. G., Springfield, J. D., Benak, R. J. & DillonH. C., Jr. H. C., Jr. (1988). Specificity of human antibodies against group B streptococcus type II. Proceedings of the Xth Lancefield Symposium (ed. R., Lutticken). New York, Stuttgart: Gustav Fischer, Verlag.Google Scholar
Gray, B. M., Springfield, J. D. & DillonH. C, Jr. H. C, Jr. (1987). Type–specific streptococcal antibodies in amniotic fluid. American Journal of Obstetrics and Oynecology 156, 666–669.CrossRefGoogle ScholarPubMed
Jennings, H. J., Rosell, K. G., Katzenllenbogen, Ed. & Kasper, D. L. (1983). Structural determination of the capsular polysaccharide antigen of type II group B streptococcus. Journal of Biological Chemistry 258, 1793–8.CrossRefGoogle ScholarPubMed
Lancefield, R. C. (1972). Cellular antigens of group B streptococci. In Streptococci and Streptococcal Diseases (ed. Wannamaker, L. W. and Matsen, J. M.), pp. 57–65. New York: Academic Press.Google Scholar
Lancefield, R. C. & Freimer, E. H. (1966). Type–specific polysaccharide antigens of group B streptococci. Journal of Hygiene 64, 191–203.CrossRefGoogle ScholarPubMed
Mayon-White, R. T. (1985). The incidence of GBS disease in neonates in different countries. Antimicrobial Chemotherapy 35, 17–27.Google ScholarPubMed
Pritchard, D. G., Egan, M. L., Gray, B. M. & DillonH. C., Jr. H. C., Jr. (1988). Immunochemical characterization of the polysaccharide antigens of group B streptococci. Reviews of Infectious Diseases. In press.Google Scholar
Wilkinson, H. W. (1978). Analysis of group B streptococcal types associated with disease in human infants and adults. Journal of Clinical Microbiology 7, 175–9.CrossRefGoogle Scholar