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Salmonella isolation from reptilian faeces: A discussion of appropriate cultural techniques

Published online by Cambridge University Press:  19 October 2009

R. W. S. Harvey
Affiliation:
Regional Public Health Laboratory, University Hospital of Wales, Heath Park, Cardiff CF4 4XW
T. H. Price
Affiliation:
Regional Public Health Laboratory, University Hospital of Wales, Heath Park, Cardiff CF4 4XW
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A short study of salmonella isolation from reptile faeces is described. The samples came from the reptile house at the Bristol Zoological Gardens. The wide range of salmonella serotypes present in the material, including representatives of all four subgenera, is noted. The important factors in the technique of isolation are discussed. These are choice of inoculum, enrichment medium, and plating Medium, useof multiple subculture and serological isolation. If the number of samples available is scanty, an extended technique will probably yield more information than a simple method.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1983

References

REFERENCES

Anon (1975). Part 1250 – control of communicable diseases. Ban on sale and distribution of small turtles. Federal Register 40, 2254322546. Quoted by Chiodini & Sundberg (1981).Google Scholar
Anon (1981). Reptilian salmonellosis. Lancet ii, 130.Google Scholar
Bailey, W. R. & Laidley, R. (1955). A method of separating mixed cultures of salmonellae. Canadian Journal of Microbiology 1, 288.CrossRefGoogle ScholarPubMed
Chau, P. Y. & Leung, Y. K. (1978). Inhibitory action of various plating media on the growth of certain Salmonella serotypes Journal of Applied Bacteriology 45, 341.CrossRefGoogle ScholarPubMed
Chiodini, R. J. & Sundbeuo, J. P. (1981). Salmonellosis in reptiles: a review. American Journal of Epidemiology 113, 494.CrossRefGoogle ScholarPubMed
Edel, W. & Kampelmacher, E. H. (1909). Salmonella isolation in nine European laboratories using a standardized technique. Bulletin of the World Health Organization 41, 297.Google Scholar
Erdman, I. E. (1974). ICMSF methods studies. IV. International collaborative assay for the detection of Salmonella in raw meat. Canadian Journal of Microbiology 20, 715.CrossRefGoogle ScholarPubMed
Habermalz, D. & Pietzsch, O. (1973). Identification of Arizona bacteria. A contribution to the problem of salmonella infections among reptiles and amphibians in zoologicalgardens. Zentralblattfiir Bakteriologie Parasitenkunde Infeklionskrankheiten und Hygiene (I. Abteilung, Originale A) 225, 323.Google Scholar
Harvey, R. W. S. (1956). Choice of a selective medium for the routine isolation of members of the salmonella group. Monthly Bulletin of the Ministry of Health and the PublicHealth imboratory Service 15, 118.Google ScholarPubMed
Harvey, R. W. S. (1957). The epidemiological significance of sewage bacteriology. British Journal of Clinical Practice 11, 751.Google ScholarPubMed
Harvey, R. W. S. (1965). A study of factors governing the isolation of salmonellas from infected materials and the application of improved techniques to epidemiological problems. M.D. Thesis, University of Edinburgh.Google Scholar
Harvey, R. W. S. (1971). Salmonellosis. In University of Nottingham Nutrition Conference for Feed Manufacturers (ed. Swan, and Lewis, ), part 5, p. 100. Edinburgh and London: Churchill Livingstone.Google Scholar
Harvey, R. W. S. & Price, T. H. (1962). Salmonella serotypes and Arizona paracolons isolated from Indian crushed bone. Monthly Bulletin of the Ministry of Health and the Public Health Laboratory Service 21, 54.Google ScholarPubMed
Harvey, R. W. S. & Price, T. H. (1974). Isolation of Salmonellas. Public Health Laboratory Service Monograph Series 8. London: HMSO.Google Scholar
Harvey, R. W. S. & Price, T. H. (1976). Isolation of salmonellas from sewage-polluted river water using selenite F and Muller-Kauffmann tetrathionate. Journal of Hygiene 77, 333.CrossRefGoogle ScholarPubMed
Harvey, R. W. S. & Price, T. H. (1982 a). Salmonella isolation and identification techniques alternative to the standard method. In Isolation and Identification Methods for Food Poisoning Organisms (Society for Applied Bacteriology Technical Series), no. 17, p. 51. London Academic Press.Google Scholar
Harvey, R. W. S. & Price, T. H. (1982 b). A comparison of the R25 modification of Rappaport's enrichment medium with strontium chloride B for salmonella isolation fromsewage polluted natural water. Journal of Hygiene 89, 111.CrossRefGoogle Scholar
Harvey, R. W. S., Price, T. H. & Hall, M. L. M.(1973). Isolation of Subgenus III salmonellas (Arizonas) in Cardiff 1959–1971. Journal of Hygiene 71, 481.CrossRefGoogle ScholarPubMed
Harvey, R. W. S., Price, T. H. & Morgan, J. (1977). Salmonella surveillance with reference to pigs – Cardiff abattoir 1968–1975. Journal of Hygiene 78, 439.CrossRefGoogle ScholarPubMed
Hinshaw, W. R. & McNeil, E. (1944). Gopher snakes as carriers of salmonellosis and paracolon infections. Cornell Veterinarian 34, 248.Google Scholar
Iveson, J. B. (1971). Strontium chloride B and EE enrichment broth medium for the isolation of Edwardsiella, Salmonella and Arizona species from tiger snakes. Journal of Hygiene 69, 323.CrossRefGoogle ScholarPubMed
Jameson, J. E. (1966). Differentiation of salmonella strains by colonial morphology. Journal of Pathology and Bacteriology 91, 141.CrossRefGoogle ScholarPubMed
Juenker, A. P. (1957). Infections with multiple types of salmonellae. American Journal of Clinical Pathology 27, 646.CrossRefGoogle ScholarPubMed
Kauffmann, F. (1930). Ein kombiniertes Anreicherungsverfahren fur Typhus – und Paratyphusbazillen. Zentralblatt fiir Bakteriologie, Parasitenkunde Infektionskrankheiten und Hygiene (I. Abteilung, Originale) 119, 148.Google Scholar
Kauffmann, F. (1935). Weitere Erfahrungen mit dem kombinierten Anseicherungsverfahren fur Salmonellabazillen. Zeitschrift fur Hygieneund Infectionskrankheiten 117, 26.CrossRefGoogle Scholar
Kaura, Y. K., Sharma, V. K. & Singh, I. P. (1970). Hardwicke's Dabb-lizard (Uromastix hardioicki)as a reservoir of Salmonella in nature. Research in Veterinary Science 11, 390.CrossRefGoogle ScholarPubMed
Kaura, Y. K., Sharma, V. K., Singh, I. P., Sakazaki, R. & Rhode, R. (1972). Snakes as reservoirs of Arizona and Salmonella. Zentralblatt für Bakteriologie Parasitenkunde and Infektionskrankheiten und Hygiene(I. Abteilung, Originale A) 219, 506.Google ScholarPubMed
Koopman, J. P. & Janssen, F. G. J. (1973). The occurrence of salmonellas and lactose-negative Arizonas in reptiles in the Netherlands and a comparison of three enrichment methods used in their isolation. Journal of Hygiene 71, 363.CrossRefGoogle Scholar
Leifson, E. (1936). New selenite enrichment media for the isolation of typhoid and paratyphoid (salmonella) bacilli. American Journal of Hygiene 24, 423.Google Scholar
Loeffler, F. (1906). Der kulturelle Nachweiss der Typhusbazillen in Faeces, Erdeund Wasser mit Hilfe des Malachitgruns. Deutsche medizmische Wochenschrift 32, 289.CrossRefGoogle Scholar
Lourero, J. A. (1942). A modification of Wilson and Blair's bismuth sulphite agar (stabilized stock solutions). Journal of Hygiene 42, 224.CrossRefGoogle Scholar
McInnes, H. M. (1971). Salmonella saint-paul infection with lizards as possible reservoir. New Zealand Veterinary Journal 19, 163.Google Scholar
Muller, L. (1923). Un nouveau milieu d'enrichissement pour la recherche du bacille typhique et des paratyphiques. Comptes Rendus des Seances de la Societe de Biologie etdes ses Filiales 89, 434.Google Scholar
Siebeling, R. J., Neal, P. M. & Granherry, W. D. (1975). Evaluation of methods for the isolation of Salmonella and Arizona organisms from pet turtles treated with antimicrobial agents. Applied Microbiology 29, 240.CrossRefGoogle ScholarPubMed
Smith, H. W. (1952). The evaluation of culture media for the isolation of salmonellae from faeces. Journal of Hygiene 50, 21.CrossRefGoogle ScholarPubMed
Van Schothorst, M., Van Leusden, F. M., Jeunink, J. & De Dreu, J. (1977). Studies on the multiplication of salmonellas in various enrichment media at different incubation temperatures. Journal of Applied Bacteriology 42, 157.CrossRefGoogle ScholarPubMed
Vassiliadis, P. (1968). Shigella spp., Salmonella cholerae-suis and Arizona in Rappaport's medium. Journal of Applied Bacteriology 31, 367.CrossRefGoogle Scholar
Vassiliadis, P., Pateraki, B. J. & Trichopoulos, D. (1974). Evaluation of the growth of salmonellao in Rappaport's broth and in Muller— Kauffmann'stetrathionato broth. Journal of Applied Bacteriology 37, 411.CrossRefGoogle ScholarPubMed
Wassén, A. (1930). Sur une methode d'enrichissement des Bacilles paratyphiques basee sur le mobilite et l'agglutination direct des Bacilles dans le milieu. Comptes Itendus des Seances de la Société de Biologie et des ses Filiales 104, 523.Google Scholar
Wells, J. G., Clark, G. M. & Morris, G. K. (1974). Evaluation of methods for isolating Salmonella and Arizona organisms from pet turtles. Applied Microbiology 27, 8.CrossRefGoogle ScholarPubMed