Hostname: page-component-77c89778f8-7drxs Total loading time: 0 Render date: 2024-07-18T06:18:40.520Z Has data issue: false hasContentIssue false
  • English
  • Français

Recognition of adenovirus types in faecal samples by Southern hybridization in South Australia

Published online by Cambridge University Press:  15 May 2009

L. D. Mickan  and
T.-W. Kok
L. D. Mickan*
Affiliation:
Division of Medical Virology, Institute of Medical and Veterinary Science, Frame Road, Adelaide, SA 5000, Australia
T.-W. Kok
Affiliation:
Division of Medical Virology, Institute of Medical and Veterinary Science, Frame Road, Adelaide, SA 5000, Australia
*
* Address correspondence and requests for reprints to: L. D. Mickan. Division of Medical Virology. Institute of Medical and Veterinary Science, P.O. Box 14 Rundle Mall, Adelaide, SouthAustralia5000.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The distribution of adenovirus types in faecal samples of patients with suspected viral gastroenteritis from South Australia was determined during the 12-month period, July 1991–June 1992. There were 3299 samples tested and 226 (6·9%) were positive for adenovirus by enzyme immunoassay. Of these 226 samples. 154 (68%) were typed directly using virus DNA extracted from the faecal samples according to the Sma I, Hind III and BstEII restriction patterns and Southern hybridization analysis with pooled viral genomic DNA probes. In this group, 86% of the samples were from patients who were < 3 years of age. Enteric adenovirus types 40 and 41 accounted for 20 and 40% respectively, of these samples, and types 1, 2, 3, 5, 6, 7 and 31 comprised the remainder. Type 40 was detected mainly in the winter and spring periods, and type 41 predominated in the autumn period. The majority of the non-enteric types were found during the late winter and spring periods.

Type
Research Article
Information
Epidemiology & Infection , Volume 112 , Issue 3 , June 1994 , pp. 603 - 613
Copyright
Copyright © Cambridge University Press 1994

References

REFERENCES

1.Brandt, CD, Kim, HW, Rodriguez, WJ et al. , Adenoviruses and paediatric gastroenteritis. J Infect Dis 1985; 151: 437–43.CrossRefGoogle Scholar
2.Uhnoo, I, Wadell, G, Svennson, L, Johansson, M. Importance of enteric adenoviruses 40 and 41 in acute gastroenteritis in infants and young children. J Clin Microbiol 1984; 20: 365–72.CrossRefGoogle ScholarPubMed
3.Brown, M. Laboratory identification of adenoviruses associated with gastroenteritis in Canada from 1983 to 1986. J Clin Microbiol 1990; 28: 1525–9.CrossRefGoogle ScholarPubMed
4.Graham, FL, Smiley, F, Russell, WC, Nairn, R. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J Gen Virol 1977; 36: 59–71.CrossRefGoogle ScholarPubMed
5.Adrian, T, Wadell, G, Hierholzer, JC, Wigand, R. DXA restriction analysis of adenovirus prototypes 1 to 41. Arch Virol 1986; 91: 277–99.CrossRefGoogle Scholar
6.Kok, T-W, Burrell, CJ. Comparison of five enzyme immunoassays, electron microscopy and latex agglutination for the detection of rotavirus in faecal specimens. J Clin Microbiol 1989; 27: 364–6.CrossRefGoogle Scholar
7.Kok, T-W, Varkanis, G, Marmion, BP, Martin, J, Esterman, A. Laboratory diagnosis of Mycoplasma pneumoniae infection. 1. direct detection of antigen in respiratory exudates by enzyme immunoassay. Epidemiol Infect 1988; 101: 669–84.CrossRefGoogle ScholarPubMed
8.Li, P, Bellett, AJD, Parish, CR. A comparison of the terminal proteins and hexon polypeptides of avian and human adenoviruses. J Gen Virol 1983; 64: 1375–9.CrossRefGoogle ScholarPubMed
9.Hirt, B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol 1967; 26: 365–9.CrossRefGoogle ScholarPubMed
10.McMinn, PC, Stewart, J, Burrell, CJ. A community outbreak of epidemic keratoconjunctivitis in central Australia due to Adenovirus type 8. J Infect Dis 1991; 164: 1113–18.CrossRefGoogle ScholarPubMed
11.Sambrook, J, Fritsch, EF, Maniatis, T. Molecular cloning. A laboratory manual, 2nd ed.Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989.Google Scholar
12.Lanser, JA, Adams, M, Doyle, R, Sangster, N, Steele, TW. Genetic relatedness of Legionella longbeachae isolates from human and environmental sources in Australia. Appl Environ Microbiol 1990; 56: 2784–90.CrossRefGoogle ScholarPubMed
13.Wadell, G. Adenoviruses. In: Principles and practice of clinical virology. Zuckerman, AJ. Banatvala, JE, Pattison, JR, ed. Chichester: John Wiley & Sons, 2nd ed. 1990: 251–74.Google Scholar
14.Willcocks, MM, Carter, MJ, Laidler, FR, Madeley, CR. Restriction enzyme analysis of faecal adenoviruses in Newcastle upon Tyne. Epidemiol Infect 1988; 101: 445–58.CrossRefGoogle ScholarPubMed
15.van der Avoort, HGAM, Wemenbol, AG, Zomerdijk, TPL et al. , Characterization of fastidious adenovirus types 40 and 41 by DNA restriction enzyme analysis and by neutralizing monoclonal antibodies. Virus Res 1989; 12: 139–58.CrossRefGoogle ScholarPubMed
16.Kidd, AH. Genome variants of adenovirus 41 (subgroup G) from children with diarrhoea in South Africa. J Med Virol 1984; 14: 49–59.CrossRefGoogle ScholarPubMed
17.Ushijima, H. Eshita, Y, Araki, K, Shinozaki, T, Togo, T, Matsunaga, Y. A study of adenovirus gastroenteritis in the Tokyo area. Eur J Pediatr 1988; 147: 90–2.CrossRefGoogle ScholarPubMed
18.Suzuki, N. Ueno, T, Yamashita, T, Fujinaga, K. Grouping of adenoviruses and identification of restriction endonuclease cleavage patterns of adenovirus DNA using infected cell DNA: simple and practical methods. Microbiol Immunol 1981; 25: 1291–301.CrossRefGoogle ScholarPubMed
19.Scott-Taylor, T. Ahluwalia, G, Klisko, B, Hammond, GW. Prevalent enteric adenovirus variant not detected by commercial monoclonal antibody enzyme immunoassay. J Clin Microbiol 1990; 28: 2797–801.CrossRefGoogle Scholar
20.De Jong, JC. Bijlsma, K. Wermenbol, AG et al. , Detection, typing, and subtyping of enteric adenoviruses 40 and 41 from fecal samples and observation of changing incidences of infections with these types and subtypes. J Clin Microbiol 1993; 31: 1562–9.CrossRefGoogle Scholar
21.Shinozaki, T. Araki, K, Fujita, Y, Kobayashi, M, Tajima, T, Abe, T. Epidemiology of enteric adenoviruses 40 and 41 in acute gastroenteritis in infants and young children in the Tokyo area. Scand J Infect Dis 1991; 23: 543–7.CrossRefGoogle ScholarPubMed
22.Bates, PR. Bailey, AS, Wood, DJ, Morris, DJ, Couriel, JM. Comparative epidemiology of rotavirus. subgenus F (types 40 and 41) adenovirus, and astrovirus gastroenteritis in children. J Med Virol 1993; 39: 224–8.CrossRefGoogle ScholarPubMed
23.Hyypia, T. Detection of adenovirus in nasopharyngeal specimens by radioactive and nonradioactive DNA probes. J Clin Microbiol 1985; 21: 730–3.CrossRefGoogle ScholarPubMed
24.Cruz, JR. Caceres, P, Cano, F. Flores, J. Bartlett, A, Torun, B. Adenovirus types 40 and 41 and rotaviruses associated with diarrhoea in children from Guatemala. J Clin Microbiol 1990; 28: 1780–4.CrossRefGoogle ScholarPubMed