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The reaction of H-substance with rabbit antisera

Published online by Cambridge University Press:  15 May 2009

R. G. S. Johns  and
J. R. Marrack
R. G. S. Johns
Affiliation:
Department of Chemical Pathology, London Hospital Medical College, Whitechapel, E. 1
J. R. Marrack
Affiliation:
Department of Chemical Pathology, London Hospital Medical College, Whitechapel, E. 1
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Quantitative methods have been used in several investigations by Kabat and his colleagues (1945–50) in the study of serological relations of various preparations of blood-group substances. The investigations have taken twoforms: (1) the amounts of antibody nitrogen precipitated from a standard volume of an antiserum by varying amounts of the blood-group substances have been plotted against the amount of blood-group substance added; (2) the hexosamine has been measured in the precipitates formed with antiserum in the zone of antibody excess and the ratio of hexosamine precipitated to hexosamine in the blood-group substance added has been calculated; the amount of methyl-pentose precipitated has also been measured (Kabat, Baer &Knaub, 1949) and the ratio of methyl-pentose precipitated to methyl-pentose added hasbeen calculated.

Type
Research Article
Information
Journal of Hygiene , Volume 51 , Issue 1 , March 1953 , pp. 55 - 63
Copyright
Copyright © Cambridge University Press 1953

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