Hostname: page-component-848d4c4894-8bljj Total loading time: 0 Render date: 2024-06-17T02:21:01.406Z Has data issue: false hasContentIssue false
  • English
  • Français

The production of mastitis in cows by the intramammary inoculation of T-mycoplasmas

Published online by Cambridge University Press:  19 October 2009

R. N. Gourlay ,
C. J. Howard  and
J. Brownlie
R. N. Gourlay
Affiliation:
A.R.C. Institute for Research on Animal Diseases, Compton, Newbury, Berkshire
C. J. Howard
Affiliation:
A.R.C. Institute for Research on Animal Diseases, Compton, Newbury, Berkshire
J. Brownlie
Affiliation:
A.R.C. Institute for Research on Animal Diseases, Compton, Newbury, Berkshire
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Six milking cows were inoculated with bovine and human T-mycoplasmas and control materials into the udder via the teat canal. Control materials produced only a slight transient cell response in the milk. Bovine T-mycoplasmas produced clinical mastitis in nine out of ten quarters inoculated. Seven developed clinical mastitis together with visible changes in the milk, excretion of T-mycoplasmas and greatly increased cell counts in the milk. In three of these quarters, in two different cows, milk secretion ceased completely. Two quarters in a different cow showed visible milk changes, excretion of T-mycoplasmas and increased cell counts. Two quarters were inoculated with human T-mycoplasmas and neither produced any signs of mastitis.

Infection of the udder with T-mycoplasmas did not stimulate high-titre serum antibody levels as measured by the metabolic inhibition test, but whey samples gave high titres in two of the cows that were able to control and resolve the infection.

Type
Research Article
Information
Journal of Hygiene , Volume 70 , Issue 3 , September 1972 , pp. 511 - 521
Copyright
Copyright © Cambridge University Press 1972

References

REFERENCES

Broadhurst, J. & Paley, C. (1930). A single-dip stain for the direct examination of milk. Journal of the American Veterinary Medical Association 94, 525.Google Scholar
Connole, M. D., Laws, L. & Hart, R. K. (1967). Mastitis in cattle caused by a Mycoplasma sp. Australian Veterinary Journal 43, 157.CrossRefGoogle ScholarPubMed
Davidson, I. & Stuart, P. (1960). Isolation of a mycoplasma-like organism from an outbreak of bovine mastitis. Veterinary Record 72, 766.Google Scholar
Gourlay, R. N. (1968). The isolation of T-strains of mycoplasma from pneumonic calf lungs. Research in Veterinary Science 9, 376.CrossRefGoogle ScholarPubMed
Gourlay, R. N. & Leach, R. H. (1970). A new mycoplasma species isolated from pneumonic lungs of calves (Mycoplasma dispar sp. nov.). Journal of Medical Microbiology 3, 111.CrossRefGoogle ScholarPubMed
Gourlay, R. N., Mackenzie, A. & Cooper, J. E. (1970). Studies of the microbiology and pathelogy of pneumonic lungs of calves. Journal of Comparative Pathelogy 80, 575.CrossRefGoogle ScholarPubMed
Gourlay, R. N. & Thomas, L. H. (1960). The isolation of large colony and T-strain mycoplasmas from cases of bovino korato-conjunctivitis. Veterinary Record 84, 416.CrossRefGoogle Scholar
Gourlay, R. N. & Thomas, L. H. (1970). The experimental production of pneumonia in calves by the ondobronchial inoculation of T-mycoplasmas. Journal of Comparative Pathelogy 80, 585.CrossRefGoogle ScholarPubMed
Hale, H. H., Helmboldt, C. F., Plastridge, W. N. & Stula, E. F. (1962). Bovino mastitis caused by a Mycoplasma species. Cornell Veterinarian 52, 682.Google Scholar
Jain, N. C., Jasper, D. E. & Dellinger, J. D. (1960). Experimental bovino mastitis due to Mycoplasma. Cornell Veterinarian 59, 10.Google Scholar
Knudsin, R. B.Driscoll, S. G. & Ming, P. L. (1967). Strain of mycoplasma associated with human reproductive failure. Science, N.Y. 157, 1573.Google Scholar
Langer, P. H. & Carmichael, L. E. (1963). Identification of pneumoenteritis isolates from cattle as Mycoplasma. Proceedings of the tilth Meeting of the U.S. Livestock Sanitary Association, p. 129.Google Scholar
Leach, R. H. (1967). Comparative studies of mycoplasma of bovino origin. Annals of the New York Academy of Science 143, 305.CrossRefGoogle Scholar
Pattison, I. H. & Holman, H. H. (1951). Studies on experimental streptococcal mastitis. IV. the disease produced by the inoculation of Streptococcus agalactiae strain S13 into the teat canal of goats. Journal of Comparative Pathelogy 61, 26.CrossRefGoogle Scholar
Purcell, R. H., Taylor-Robinson, D., Wong, D. & Chanock, R. M. (1960). Colour test for the measurement of antibody to T-strain mycoplasmas. Journal of Bacteriology 92, 6.CrossRefGoogle Scholar
Shepard, M. C. (1954). The recovery of plouropneumonia-like organisms from Negro men with and witheut non-gonococcal urothritis. American Journal of Syphilis, Gonorrhea and Venereal Diseases 38, 113.Google Scholar
Shepard, M. C. (1969). Fundamental biology of the T-strains. In The Mycoplasmatales and the L'phase of Bacteria. Ed. Hayflick, L.. Amsterdam: North Holland Publishing Company.Google Scholar
Spooner, R. L. & Miller, J. K. (1971). The measurement of haemoglobin reactivo protein in ruminants as an aid to the diagnosis of acuto inflammation. Veterinary Record 88, 2.CrossRefGoogle Scholar
Stuart, P., Davidson, I., Slavin, G., Edgson, F. A. & Howell, D. (1963). Bovino mastitis caused by mycoplasma. Veterinary Record 75, 59.Google Scholar
Tan, R. J. S. & Markham, J. G. (1971). Folino T-strain mycoplasmas. Japanese Journal of Experimental Medicine 41, 247.Google Scholar
Taylor-Robinson, D., Haig, D. A. & Williams, M. H. (1967). Bovino T-strain mycoplasma. Annals of the New York Academy of Science 143, 517.CrossRefGoogle Scholar