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Prevention of low temperature denaturation injury in T4Bo phage by low concentrations of traditional cryoprotective additives

Published online by Cambridge University Press:  15 May 2009

P. R. M. Steele
P. R. M. Steele
Affiliation:
University Department of Pathology, Cambridge, England
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The well known cryoprotective additives glucose, sucrose, glycerol, dimethyl sulphoxide, polyvinylpyrrolidone, dextran and ammonium acetate have been found to prevent inactivation of T4Bo phage frozen in sodium bromide solutions. Their protective effects in this experimental system could not be accounted for by a colligative mechanism. It is proposed that they may act by modifying the structure of the unfrozen aqueous phase rather than by direct interaction with the phage.

Type
Research Article
Information
Journal of Hygiene , Volume 76 , Issue 3 , June 1976 , pp. 453 - 458
Copyright
Copyright © Cambridge University Press 1976

References

REFERENCES

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