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Prevalence of antibodies against Entamoeba histolytica in Mexico measured by ELISA

Published online by Cambridge University Press:  15 May 2009

C. R. González ,
A. Isibasi ,
V. Ortiz-Navarrete ,
J. Paniagua ,
J. A. García ,
A. Ramirez ,
B. Salvatierra ,
R. Tapia ,
J. Sepúlveda  and
G. Gutiérrez
...Show all authors
C. R. González
Affiliation:
Laboratorio de Inmunoquímica, Unidad de Investigatión Biomedica, Centra Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Apartado Postal 73-032, México, D.F. 03020, México
A. Isibasi*
Affiliation:
Laboratorio de Inmunoquímica, Unidad de Investigatión Biomedica, Centra Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Apartado Postal 73-032, México, D.F. 03020, México
V. Ortiz-Navarrete
Affiliation:
Laboratorio de Inmunoquímica, Unidad de Investigatión Biomedica, Centra Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Apartado Postal 73-032, México, D.F. 03020, México
J. Paniagua
Affiliation:
Laboratorio de Inmunoquímica, Unidad de Investigatión Biomedica, Centra Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Apartado Postal 73-032, México, D.F. 03020, México
J. A. García
Affiliation:
Laboratorio de Inmunoquímica, Unidad de Investigatión Biomedica, Centra Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Apartado Postal 73-032, México, D.F. 03020, México
A. Ramirez
Affiliation:
Laboratorio de Inmunoquímica, Unidad de Investigatión Biomedica, Centra Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Apartado Postal 73-032, México, D.F. 03020, México
B. Salvatierra
Affiliation:
Secretanía de Salud. Av. Reforma esq. Lieja, México, D.F. 06696., México
R. Tapia
Affiliation:
Secretanía de Salud. Av. Reforma esq. Lieja, México, D.F. 06696., México
J. Sepúlveda
Affiliation:
Secretanía de Salud. Av. Reforma esq. Lieja, México, D.F. 06696., México
G. Gutiérrez
Affiliation:
Secretanía de Salud. Av. Reforma esq. Lieja, México, D.F. 06696., México
J. Kumate
Affiliation:
Secretanía de Salud. Av. Reforma esq. Lieja, México, D.F. 06696., México
*
* Corresponding author.
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The prevalence of antibodies against Entamoeba histolytica was studied in the Mexican population using an immunoenzyme assay in solid phase (ELISA) and semiautomatic equipment. The antigen was a mixture of membrane proteins obtained by Triton X-100 extraction from an axenic culture of Entamoeba histolytica HM1-IMSS. The method was standardized by comparing serum samples from amoebic liver abscess patients with healthy volunteers. From the 60538 samples supplied by the National Seroepidemiology Survey, antibodies were found in 4·49 % (4·32–;4·65% at 95 % confidence limit). More significant titres occurred in the central region of the country. The ratio female to male was 1·25:1. The population living in metropolitan areas had probably been infected at a younger age than those living in the country.

Important differences were found in the seroprevalence obtained by ELISA compared with a study which used indirect haemagglutination (IHA) in the same sample frame.

Type
Research Article
Information
Epidemiology & Infection , Volume 115 , Issue 3 , December 1995 , pp. 535 - 543
Copyright
Copyright © Cambridge University Press 1995

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