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Plasmid content, auxotype and protein-I serovar of gonococci isolated in the Gambia

Published online by Cambridge University Press:  19 October 2009

A. P. Johnson ,
D. Abeck ,
R. A. Wall ,
D. C. W. Mabey  and
D. Taylor-Robinson
A. P. Johnson
Affiliation:
Division of Sexually Transmitted Diseases
D. Abeck
Affiliation:
Division of Sexually Transmitted Diseases
R. A. Wall
Affiliation:
Microbial Pathogenicity Research Group, MRC Clinical Research Centre, Watford Road, Harrow, Middlesex HA1 3UJ, MRC Laboratories, Fajara, The Gambia
D. C. W. Mabey
Affiliation:
Microbial Pathogenicity Research Group, MRC Clinical Research Centre, Watford Road, Harrow, Middlesex HA1 3UJ
D. Taylor-Robinson
Affiliation:
Division of Sexually Transmitted Diseases
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Summary

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Twenty-nine strains of penicillinase-producing Neisseria gonorrhoeae (PPNG) and 30 non-penicillinase-producing strains, all isolated in the Gambia, were characterized in terms of their plasmid content, auxotype and protein-I serovar. Sixty-two per cent of the PPNG strains contained the 3·2 MDa penicillinasecoding plasmid, and 38% had the 4·4 MDa plasmid. All the PPNG strains contained the 2·6 MDa cryptic plasmid but lacked the 24·4 MDa conjugative plasmid. In contrast, 46·7% of the non-PPNG strains harboured only the cryptic plasmid while 16·7% contained both the cryptic and conjugative plasmids. Seventeen per cent of the non-PPNG strains contained the conjugative plasmid only and 20% lacked plasmids.

The PPNG and non-PPNG strains also differed in terms of their protein-I serovar. Eighty-six per cent of the PPNG strains belonged to serogroup 1A, whereas the majority (60%) of non-PPNG strains belonged to serogroup IB. There was no significant difference in the auxotypes of the PPNG and non-PPNG strains, with both groups consisting predominantly of prototrophic and prolinerequiring strains, with a minority of strains requiring arginine. When the 59 strains were each characterized in terms of their combined plasmid profile, auxotype and serovar, 39 different combinations were noted, which indicates the heterogeneous nature of the gonococcal population found in the Gambia.

Type
Research Article
Information
Epidemiology & Infection , Volume 99 , Issue 3 , December 1987 , pp. 669 - 674
Copyright
Copyright © Cambridge University Press 1987

References

REFERENCES

Anderson, B., Odugbemi, T. & Johnson, S. (1982). Penicillinase-producing Neisseria gonorrhoeae from Nigeria with Far Eastern type plasmid. Lancet i, 676.Google Scholar
Bello, C. S. S., Lloyd-Evans, N., McRobbie, I., Mabey, D. C. W. & Odugbemi, T. O. (1985). Characterisation of Neisseria gonorrheae isolates from the Gambia, West Africa. European Journal of Sexually Transmitted Diseases, 2, 229.Google Scholar
Birnboim, H. C. & Doly, J. A. (1979). A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acid Research 7, 1513–1523.Google Scholar
Bogaerts, J., Vandepitte, J., Van Dyck, E., Vanhoof, R., Dekegel, M. & Piot, P. (1986). In vitro antimicrobial sensitivity of Neisseria gonorrhoeae from Rwanda. Genitourinary Medicine 62, 217–220.Google Scholar
Catlin, B. W. (1973). Nutritional profiles for Neisseria gonorrhoeae, Neisseria meningitidis and Neisseria lactamica in chemically defined media, and the use of growth requirements for gonococcal typing. Journal of Infectious Diseases 122, 173–194.Google Scholar
Dillon, J.-A. & Pauze, M. (1981). Relationship between plasmid content and auxotype in Neisseria gonorrhoeae isolates. Infection and Immunity 19, 952–957.Google Scholar
Dillon, J.-A., Duck, P. & Thomas, D. Y. (1981). Molecular and phenotypic characterisation of penicillinase-producing Neisseria gonorrhoeae from Canadian sources. Antimicrobial Agents and Chemotherapy 19, 952–957.Google Scholar
Handsfield, H. H., Sandström, E. G., Knapp, J. S., Perine, P. L., Whittington, W. L., Sayers, D. E. & Holmes, K. K. (1982). Epidemiology of penicillinase-producing Neisseria gonorrhoeae infections. Analysis by auxotyping and serogrouping. New England Journal of Medicine 16, 950–954.CrossRefGoogle Scholar
Knapp, J. S., Tam, M. R., Nowinski, R. C., Holmes, K. K. & Sandström, E. G. (1984). Serological classification of Neisseria gonorrhoeae with use of monoclonal antibodies to gonococcal outer membrane protein I. Journal of Infectious Diseases 150, 44–48.CrossRefGoogle ScholarPubMed
O'Callaghan, C. H., Morris, A., Kirby, S. M. & Shingler, A. H. (1972). Novel method for detection of β-Mactamase by using a chromogenic cephalosporin substrate. Antimicrobial Agents and Chemotherapy 1, 283–288.Google Scholar
Osoba, A. O., Johnston, N. A., Ogunbanjo, B. O. & Ochei, J. (1987). Plasmid profile of Neisseria gonorrhoeae in Nigeria and efficacy of spectinomycin in treating gonorrhea. Genitourinary Medicine 63, 1–5.Google Scholar
Perine, P. L., Thornsbery, C., Schalla, W., Biddle, J., Siegel, M. S., Wong, K.-H. & Thompson, S. E. (1977). Evidence for two distinct types of penicillinase-producing Neisseria gonorrhoeae. Lancet ii, 993–995.CrossRefGoogle Scholar
Perine, P. L., Totten, P. A., Knapp, J. S., Holmes, K. K., Bentsi, C. & Klufio, C. A. (1983). Diversity of gonococcal plasmids, auxotypes and serogroups in Ghana. Lancet i, 1051–1052.Google Scholar
Plummer, F. A., D'Costa, L. J., Nsanze, H., Slaney, L., Dewitt, W. E., Knapp, J. S., Dillon, J.-A., Albritton, W. L. & Ronald, A. R. (1985). Development of endemic penicillinase-producing Neisseria gonorrhoeae in Kenya. In The Pathogenic Neisseriae (ed. Schoolnik, G. K.), pp. 101–106. Washington, D.C.: American Society for Microbiology.Google Scholar
Roberts, M. & Falkow, S. (1977). Conjugal transfer of R plasmids in Neisseria gonorrhoeae. Nature 266, 630–631.Google Scholar
Roberts, M., Piot, P. & Falkow, S. (1979). The ecology of gonococcal plasmids. Journal of General Microbiology 114, 491–494.CrossRefGoogle ScholarPubMed
Swinscow, T. D. V. (1976). Statistics at square one. XIV. The x 2 test. British Medical Journal ii, 462–463.Google Scholar
Totten, P. A., Holmes, K. K., Handsfield, H. H., Knapp, J. S., Perine, P. L. & Falkow, S. (1983). DNA hybridisation technique for the detection of Neisseria gonorrhoeae in men with urethritis. Journal of Infectious Diseases 148, 462–471.CrossRefGoogle ScholarPubMed