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Monoclonal antibodies directed against the flagella of Campylobacter jejuni: production, characterization and lack of effect on the colonization of infant mice

Published online by Cambridge University Press:  19 October 2009

D. G. Newell
D. G. Newell
Affiliation:
Experimental Pathology Laboratory, PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire
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Summary

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Eight monoclonal antibodies have been derived from Balb/e mice hyperimmunized with the purified flagella from Campylobacter jejuni strain 81116. These monoclonal antibodies are directed against flagella as demonstrated by reaction in ELISA against flagellate and aflagellate antigens, radio-immunoprecipitation and electro-immunoblotting techniques. Some of the antibodies react with a 60K minor protein as well as the 62K flagella protein. This protein may be related to an antigen expressed on the surface of the organism and detectable by immunogold labelling with one of the monoclonal antibodies.

None of the antibodies causes the aggregation of bacteria or inhibits bacterial motility, unlike polyclonal anti-flagella antiserum. Moreover, none of the antibodies tested protected infant mice from colonization with C. jejuni strain 81116 even though partial protection (28%) was observed with syngeneic anti-flagella antiserum. Absence of protection is probably due to the cryptic nature of the flagella epitopes investigated or lack of antibody activity in the gastrointestinal tract.

Type
Research Article
Information
Journal of Hygiene , Volume 96 , Issue 2 , April 1986 , pp. 131 - 141
Copyright
Copyright © Cambridge University Press 1986

References

REFERENCES

De St Groth, S. F. & Scheidegger, D. (1980). Production of monoclonal antibodies: Strategy and tactics. Journal of Immunological Methods 35, 1–21.CrossRefGoogle ScholarPubMed
Dolby, J. M. & Newell, D. G. (1985). The protection of infant mice from colonization with Campylobacter jejuni by vaccination of the dams. Journal of Hygiene (In the Press.)Google Scholar
Field, L. H., Underwood, J. L., Pope, L. M. & Berry, L. J. (1981). Intestinal colonisation of neonatal animals by Campylobacter fetus subspecies jejuni. Infection and Immunity 33, 884–892.CrossRefGoogle Scholar
Hassan, A. G. & Dolby, J. M. (1985). Mechanisms of protection of infant mice against colonisation by Campylobacter jejuni. In Campylobacter III (eds. Pearson, A., Skirrow, M. B., Lior, H. & Rowe, B.). London: P.H.L.S. (In the Press.)Google Scholar
Hodges, G. M., Smolira, M. A. & Livingston, D. C. (1984). Scanning electron microscope immunocytochemistry in practice. In Immunolabelling for Electron Microscopy (eds. Polak, & Varndell, ) pp. 189–233. Elsevier Publications.Google Scholar
Lambden, P. R., Heckels, J. E., James, L. T. & Watts, P. J. (1979). Variations in surface protein composition associated with virulence properties in opacity types of Neisseria gonorrhoeae. Journal of General Microbiology 114, 305–312.CrossRefGoogle ScholarPubMed
Logan, S. M. & Trust, T. J. (1982). Outer membrane characteristics of Campylobacter jejuni. Infection and Immunity 38, 898–906.CrossRefGoogle ScholarPubMed
Morooka, T., Umeda, A. & Amako, K. (1985). Motility as an intestinal colonisation factor for Campylobacter jejuni. Journal of General Microbiology 131, 1973–1980.Google ScholarPubMed
Nachamkin, I. & Hart, A. M. (1985). Western blot analysis of the human antibody response to Campylobacter jejuni cellular antigens during gastrointestinal infection. Journal of Clinical Microbiology 21, 33–38.CrossRefGoogle ScholarPubMed
Newell, D. G. (1984). Experimental studies of campylobacter enteritis. In Campylobacter Infection in Man and Animals (ed. Butzler, J. P.), pp. 113–131. Boca Raton: C.R.C. Press Inc.Google Scholar
Newell, D. G. (1985). Multiple flagella antigens of Campylobacler jejuni distinguished by human convalescent sera and monoclonal antibodies. In Campylobacler III (eds. Pearson, A., Skirrow, M. B., Lior, H. & Rowe, B.). London: P.H.L.S. (In the Press.)Google Scholar
Newell, D. G., McBride, H. & Pearson, A. (1984). The identification of outer membrane proteins and flagella of Campylobacter jejuni. Journal of General Microbiology 130, 1201–1208.Google ScholarPubMed
Newell, D. G., McBride, H. & Dolby, J. M. (1985). The role of flagella in the pathogenesis of Campylobacter jejuni. Journal of Hygiene 95, 217–227.CrossRefGoogle ScholarPubMed
Newell, D. G. & Pearson, A. (1984). The invasion of epithelial cell lines and the intestinal epithelium of infant mice by Campylobacter jejuni/coli. Journal of Diarrhoeal Disease Research 2, 19–26.Google ScholarPubMed
Skirrow, M. B. (1983). Clinical aspects: infections due to C. jejuni and C. coli. In Campylobacter II (eds. Pearson, A., Skirrow, M. B., Rowe, B., Davies, J. R. & Jones, D. M.), pp. 6–7, London: P.H.L.S.Google Scholar
Wendow, W. M., Chai, J., Louie, T. J., Goudreau, C., Lior, H., Newell, D. G., Pearson, A. D. & Taylor, D. E. (1985). Antigenic analysis of Campylobacter flagellar protein and other proteins. Journal of Clinical Microbiology 21, 108–112.Google Scholar