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Molecular epidemiology of Salmonella enteritidis phage type 1b and 6a isolates in Portugal

Published online by Cambridge University Press:  23 September 2003

A. R. SOARES  and
J. MACHADO
A. R. SOARES
Affiliation:
Enterobactereaceae Unity, Bacteriology Center, National Institute of Health Dr. Ricardo Jorge, Lisbon, Portugal
J. MACHADO
Affiliation:
Enterobactereaceae Unity, Bacteriology Center, National Institute of Health Dr. Ricardo Jorge, Lisbon, Portugal
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Abstract

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Salmonella enterica serotype Enteritidis is an important serovar comprising 76% of salmonella isolates in Portugal in 2001.

For better understand the epidemiology of salmonellosis, a total of 47 isolates of S. Enteritidis phage type (PT) 1b and 6a were analysed by pulsed-field gel electrophoresis (PFGE) and genomic DNA was subjected to macro restriction with XbaI. For PT1b isolates, only three different patterns were observed, and PT6a showed a total of 10 digestion patterns. Curiously, the main pattern among PT1b isolates seams quite similar to main pattern of PT6a isolates, but when the two patterns were analysed with Bionumerics, we observed that they exhibited some differences. It was concluded that, in 2001, there was one predominant pattern for PT1b and PT6a and, possibly, we were in presence of clonal strains that exists all over the country.

Type
Research Article
Information
Epidemiology & Infection , Volume 131 , Issue 1 , August 2003 , pp. 607 - 612
Copyright
© 2003 Cambridge University Press