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Isolation of Salmonella with the use of 100 ml of the R10 modification of Rappaport's enrichment medium

Published online by Cambridge University Press:  25 March 2010

P. Vassiliadis ,
D. Trichopoulos ,
V. Kalapothaki  and
Ch. Sérié
P. Vassiliadis
Affiliation:
The Hellenic Pasteur Institute, Athens-618, Greece and the Department of Hygiene and Epidemiology of the University of Athens, Goudi, Athens-609, Greece
D. Trichopoulos
Affiliation:
The Hellenic Pasteur Institute, Athens-618, Greece and the Department of Hygiene and Epidemiology of the University of Athens, Goudi, Athens-609, Greece
V. Kalapothaki
Affiliation:
The Hellenic Pasteur Institute, Athens-618, Greece and the Department of Hygiene and Epidemiology of the University of Athens, Goudi, Athens-609, Greece
Ch. Sérié
Affiliation:
The Hellenic Pasteur Institute, Athens-618, Greece and the Department of Hygiene and Epidemiology of the University of Athens, Goudi, Athens-609, Greece
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One hundred and eighty samples of pork sausages were examined after preenrichment in buffered peptone water (P medium), for the presence of salmonellas. From each pre-enrichment four enrichments were made: (1) 0·1 ml of P medium was inoculated into 10 ml of Rappaport's medium, formula R10 (R10/43 °C), (2) 1 ml of the P medium was added to 100 ml of R10 broth (R10/100 ml/43 °C), (3) 1 ml of P medium was inoculated into 10 ml of Muller-Kauffmann tetrathionate broth (MK medium) prepared in accordance with the International Standards Organization document ISO 3565 (MK/43 °C) and (4) 10 ml of P medium were added to 100 ml of MK broth (MK/100 ml/43 °C). All the enrichments were incubated at 43 °C for 48 h. Forty-six and 47 samples were found positive with the first two enrichment methods (R10/43 °C and R10/100 ml/43 °C), while only 16 samples were found positive with the method MK/43 °C, and 27 with the method MK/100 ml/43 °C. The superiority of either one of the two R10 procedures over either one of the two MK methods is statistically highly significant (paired χ2; P < 0·001 in all four comparisons). The superiority of procedure MK/100 ml/43 °C over the method MK/43 °C is also statistically significant (P < 0·005). Both R10/43 °C and R10/100 ml/43 °C procedures had a much stronger inhibitory effect on the competing organisms (lactose- and sucrosenegative) than the two MK methods.

Type
Research Article
Information
Journal of Hygiene , Volume 87 , Issue 1 , August 1981 , pp. 35 - 41
Copyright
Copyright © Cambridge University Press 1981

References

Anon (1975). Meat and meat products - detection of salmonellae (Reference method). International Standard ISO 3565. Geneva, Switzerland: International Organization for Standardization.Google Scholar
Edel, W. & kampelmacher, E. H. (1969). Salmonella isolation in nine European laboratories using a standardized technique. Bulletin of the World Health Organization 41, 297–306.Google ScholarPubMed
Edel, W. & Kampelmacher, E. H. (1973). Comparative studies on the isolation of sublethally injured salmonellae in nine European laboratories. Bulletin of the World Health Organization 48, 167–74.Google ScholarPubMed
Harvey, R. W. S. & Price, T. H. (1977). Observations on pre-enrichment for isolating salmonellas from sewage-polluted natural water using Muller-Kauffmann tetrathionate broth prepared with fresh and desiccated ox bile. Journal of Applied Bacteriology 43, 145–8.CrossRefGoogle ScholarPubMed
Harvey, R. W. S., Price, T. H. & Crone, P. B. (1975). Quality control test of two Salmonella enrichment media using different inocula. Journal of Hygiene 74, 375–84.CrossRefGoogle Scholar
Harvey, R. W. S., Price, T. H. & Hall, Mary L. M. (1973). Isolations of subgenus III salmonellas (arizonas) in Cardiff, 1959–1971. Journal of Hygiene 71, 481–6.CrossRefGoogle ScholarPubMed
Harvey, R. W. S., Price, T. H. & Xirouchaki, E. (1979). Comparison of selenite F. Muller-Kauffmann tetrathionate and Rappaport's medium for the isolation of salmonellas from sewage-polluted natural water using a pre-enrichment technique. Journal of Hygiene 83, 451–60.CrossRefGoogle ScholarPubMed
McCoy, J. H. (1962). The isolation of salmonellae. Journal of Applied Bacteriology 25, 213–24.CrossRefGoogle Scholar
Papadakis, J. A., Kalapothaki, V., Polychronopoulou, A., Trichopoulos, D. & Vassiliadis, P. (1976). Evaluation of different malachite green brands in the performance of Rappaport's medium. European Journal of Applied Microbiology 2, 135–41.CrossRefGoogle Scholar
Rappaport, F., Konforti, N. & Navos, B. (1956). A new enrichment medium for certain salmonellae. Journal of Clinical Pathology 9. 261–6.CrossRefGoogle Scholar
Trichopoulos, D., Papadakis, J. A., Karalis, D. & Vassiliadis, P. (1975). Incubation at raised temperature of enrichment media, combined with secondary enrichment in Rappaport's medium, for the isolation of salmonellas from sewage. Journal of Hygiene 74, 205–13.CrossRefGoogle ScholarPubMed
van Schothorst, M., van Leusden, F. M., Jeunink, J. & de Dreu, J. (1977). Studies on the multiplication of salmonellas in various enrichment media at different incubation temperatures. Journal of Applied Bacteriology 42. 157–63.CrossRefGoogle ScholarPubMed
Vassiliadis, P., Trichopoulos, D., Papadakis, G. & Politi, G. (1970). Salmonella isolations in abattoirs in Greece. Journal of Hygiene 68, 601–9.Google ScholarPubMed
Vassiliadis, P., Papadakis, J., Patéraki, E., Trichopoulos, D., Karabatsos, B. & Papoutsakis, G. (1972). Isolement de Salmonella à partir de carcasses de poulets par enrichissement en bouillon et enrichissement secondaire en milieu de Rappaport. Archives de l'Institut Pasteur Hellénique 18, 19–29.Google Scholar
Vassiliadis, P., Patéraki, E., Papadakis, J. & Trichopoulos, D. (1974). Evaluation of the growth of salmonellae in Rappaport's broth and in Muller-Kauffmann's tetrathionate broth. Journal of Applied Bacteriology 37, 411–18.CrossRefGoogle ScholarPubMed
Vassiliadis, P., Patéraki, E., Papaïconomou, N., Papadakis, J. A. & Trichopoulos, D. (1976). Nouveau procédé d'enrichissement de Salmonella. Annales de Microbiologie (Institut Pasteur) 127 B, 195–200.Google Scholar
Vassiliadis, P., Kalandidi, A., Xirouchaki, E., Papadakis, J. & Trichopoulos, D. (1977). Isolement de salmonelles à partir de saucisses de pore en utilisant un nouveau procédé d'enrichissement (R10/43°). Recueil de médecine vétérinaire de l'École d'Alfort 153, 489–94.Google Scholar
Vassiliadis, P., Trichopoulos, D., Patéraki, E. & Papaïconomou, N. (1978 a). Isolation of Salmonella from minced meat by the use of a new procedure of enrichment. Zentralblatt für Bakteriologie Originate B. Hygiene, Präventive Medizin 166, 81–6.Google ScholarPubMed
Vassiliadis, P., Trichopoulos, D., Kalandidi, A. & Xirouchaki, E. (1978 b). Isolation of salmonellae from sewage with a new procedure of enrichment. Journal of Applied Bacteriology 44, 233–9.CrossRefGoogle ScholarPubMed
Vassiliadis, P., Trichopoulos, D., Kalapothaki, V., Papadakis, J. & Sérié, Ch. (1979 a). Isolement de salmonelles à partir de matières fécales de pores apparamment sains, avec le nouveau procédé d'enrichissement R10/43 °. Recueil de médecine vétérinaire de l'École d'Alfort 155, 559–66.Google Scholar
Vassiliadis, P., Trichopoulos, D., Papoutsakis, G. & Pallandiou, E. (1979 b). A note on the comparison of two modifications of Rappaport's medium with selenite broth in the isolation of salmonellas. Journal of Applied Bacteriology 46, 567–9.CrossRefGoogle ScholarPubMed
Vassiliadis, P., Trichopoulos, D., Papadakis, J., Kalapothaki, V. & Sérié, Ch. (1979 c). Brilliant green deoxycholate agar as an improved selective medium for the isolation of Salmonella. Annales de la Sociélé Belge de Médecine Tropicale 59, 117–20.Google ScholarPubMed