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The isolation and characterization of foot-and-mouth disease virus from clinically normal herds of cattle in Botswana

Published online by Cambridge University Press:  15 May 2009

R. S. Hedger
R. S. Hedger
Affiliation:
The Animal Virus Research Institute, Pirbright, Surrey
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The results of a survey of the foot-and-mouth disease carrier state in three localities of an FMD enzootic area of Botswana are presented. The collection, storage and handling of the samples is mentioned and the isolation of virus on calf thyroid monolayer tissue cultures is described. Type SAT 3 virus was isolated from up to 20% of the animals sampled at periods between 7 and 12 months after natural infection. Sera were tested for all the animals from which virus was recovered and compared with similar numbers of sera from virus-negative animals. No correlation was found between the carrier state and serum antibody titres.

Antigenic studies are described which indicated not only an antigenic variation of some carrier viruses isolated from the outbreak strain but also antigenic differences between strains of carrier virus from different animals in the same herd. There was no evidence at the time of sampling of the persistence as carrier virus of the attenuated vaccine strains of FMDV used previously in the cattle sampled.

The results suggest that transference of carrier virus from animal to animal may occur in the absence of clinical infection.

Type
Research Article
Information
Journal of Hygiene , Volume 66 , Issue 1 , March 1968 , pp. 27 - 36
Copyright
Copyright © Cambridge University Press 1968

References

REFERENCES

Auge De Mello, P., Honigman, M. N. & Fernandez, M. V. (1966). Supervivencia en bovinos del virus modificado de la fiebre aftosa. Bull. Off. int. Épizoot. 65, 2091–106.Google Scholar
Van Bekkum, J. G., Frenkel, H. S., Fredericks, H. H. J. & Fhenkel, S. (1959). Observations on the carrier state of cattle exposed to foot-and-mouth disease virus. Tijdschr. Diergeneesk. 84, 1159–64.Google Scholar
Van Bekkum, J. G., Straver, P. J., Bool, P. H. & Frenkel, S. (1966). Further information on the persistence of infective FMDV in cattle exposed to virulent virus strains. Bull. Off. int. Épizoot. 65, 1949–65.Google Scholar
Burrows, R. (1966). Studies on the carrier state of cattle exposed to FMDV. J. Hyg., Camb. 64, 81–90.CrossRefGoogle Scholar
Casey, Helen L. (1965). Standardised diagnostic complement fixation method and adaptation to micro test. Publ. Hlth Monogr. 74, 1–34.Google ScholarPubMed
Davie, J. (1964). A complement fixation technique for the quantitative measurement of antigenic differences between strains of the virus of foot-and-mouth disease. J. Hyg., Camb. 62, 401–11.Google ScholarPubMed
Hedger, R. S., Bachmann, M. & Herniman, K. A. J. Immunity studies and heterotypic responses to FMD vaccination in an area enzootic to the disease. To be published.Google Scholar
Hyslop, N., St, G. & Fagg, R. H. (1965). Isolation of variants during passage of a strain of FMDV in partially immunized cattle. J. Hyg., Camb. 63, 357–68.CrossRefGoogle Scholar
Kärber, G. (1931). Beitrag zur kollectiven Behandlung pharmakologischer Reihenversuche. Arch. exp. Path. Pharmak. 162, 480–3.CrossRefGoogle Scholar
Martin, W. B. & Chapman, W. G. (1961). The tissue culture colour test for assaying the virus and neutralising antibody of FMD and its application to the measurement of immunity in cattle. Res. vet. Sci. 2, 53–61.CrossRefGoogle Scholar
Snowdon, W. A. (1966). The growth of FMDV in monolayer cultures of calf thyroid cells. Nature, Lond. 210, 1079–80.CrossRefGoogle Scholar
Sütmoller, P. & Gaggero, A. (1965). FMD carriers. Vet. Rec. 77, 986–9.Google Scholar