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Interactions of TRIC agents with macrophages and BHK-21 cells observed by electron microscopy

Published online by Cambridge University Press:  15 May 2009

A. M. Lawn ,
W. A. Blyth  and
Janice Taverne
A. M. Lawn
Affiliation:
Lister Institute of Preventive Medicine, LondonSW1W 8RH
W. A. Blyth
Affiliation:
Lister Institute of Preventive Medicine, LondonSW1W 8RH
Janice Taverne
Affiliation:
Lister Institute of Preventive Medicine, LondonSW1W 8RH
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Summary

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TRIC agents do not multiply in mouse peritoneal macrophages in culture but have a toxic effect on them, whereas they multiply readily in BHK-21 cells. Sections of macrophages and of BHK-21 cells fixed during the first 90 min after inoculation were examined by electron microscopy. Macrophages ingested all forms of the organism, which were eventually degraded in lysosomes. However, elementary bodies were distinguished from other TRIC particles by the delay in their transfer to lysosomes. BHK-21 cells ingested elementary bodies selectively, and in these cells the organisms were neither found in lysosomes nor degraded. Instead they showed morphological changes that probably represented an early stage of development.

Type
Research Article
Information
Journal of Hygiene , Volume 71 , Issue 3 , September 1973 , pp. 515 - 528
Copyright
Copyright © Cambridge University Press 1973

References

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