Hostname: page-component-5c6d5d7d68-txr5j Total loading time: 0 Render date: 2024-08-07T13:55:02.963Z Has data issue: false hasContentIssue false
  • English
  • Français

The infectivity assay of foot-and-mouth disease virus in pigs

Published online by Cambridge University Press:  15 May 2009

R. Burrows
R. Burrows
Affiliation:
The Animal Virus Research Institute, Pirbright, Surrey
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

FMDV strains were titrated in pigs by inoculation of the bulb of the heel of the foot, the coronary band region of the foot, and the tongue. Heel inoculation gave end-points approximately 300-fold higher than did the other two methods.

Parallel titrations in cattle and mice gave infectivity estimates approximately 10-fold and 100-fold higher, respectively, than did heel titration of pigs.

The average variance of individual end-points determined for heel titration was 0·46 (log10 units) and that determined for coronary-band titration was 0·74. The sources of variations in end-points given by heel titrations are discussed.

Type
Research Article
Information
Journal of Hygiene , Volume 64 , Issue 4 , December 1966 , pp. 419 - 429
Copyright
Copyright © Cambridge University Press 1966

References

REFERENCES

Brooksby, J. B., (1950). Strains of the virus of foot-and-mouth disease showing natural adaptation to swine. J. Hyg., Camb. 48, 184.CrossRefGoogle ScholarPubMed
Burrows, R., (1964 a). The behaviour of some modified strains of foot-and-mouth disease virus in the pig. I. Innocuity. Bull. Off. int. Épizoot. 61, 1251.Google Scholar
Burrows, R., (1964 b). The behaviour of some modified strains of foot-and-mouth disease virus in the pig. II. Immunogenicity. Bull. Off. int. Épizoot. 61, 1277.Google Scholar
Girard, H. C., Charutamra, U., Supavilai, P., Smitinondana, P., Punya-Upaphat, S., & Chandrakeo, T., (1963). Vaccination against foot-and-mouth disease in the pig with an Asia 1 strain vaccine. Bull. Off. int. Épizoot. 59, 1092.Google Scholar
Graves, J. H., & Cunliffe, H. R., (1960). The infectivity assay of foot-and-mouth disease virus in swine. Proc. U.S. live Stk sanit. Ass. 63, 340.Google Scholar
Heatley, W., Skinner, H. H., & Subak-Sharpe, H., (1960). Influence of route of inoculation and strain of mouse on infectivity titrations of the virus of foot-and-mouth disease. Nature, Lond. 186, 909.CrossRefGoogle Scholar
Henderson, W. M., & Brooksby, J. B., (1948). The survival of foot-and-mouth disease virus in meat and offal. J. Hyg., Camb. 46, 394.Google ScholarPubMed
Henderson, W. M., (1949). The quantitative study of foot-and-mouth disease virus. A.R.C. Sep. Ser. no. 8, London, H. M.S.O.Google Scholar
Henderson, W. M., (1952). A comparison of different routes of inoculation of cattle for detection of the virus of foot-and-mouth disease. J. Hyg., Camb. 50, 18.CrossRefGoogle ScholarPubMed
Hyslop, N. St G., & Skinner, H. H., (1964). A note on the interpretation of infectivity titres of foot-and-mouth disease virus based on cattle tongue titrations. Bull. Off. int. Épizoot. 61, 1091.Google Scholar
Komarov, A., & Goldsmit, L., (1958). Avianized modified foot-and-mouth disease viruses. Bull. Res. Coun. Israel 7E, 217.Google Scholar
Lucam, F., & Fedida, M., (1958). Une nouvelle méthode quantitative pour l'appréciation de léimmunité anti-aphteuse. Bull. Off. int. Épizoot. 49, 596.Google Scholar
Lucam, F., Dhennin, L., Dhennin, L., & Fedida, M., (1962). Essais de vaccination anti-aphteuse intradermique chez le pore. Résultats obtenus au laboratoire. Bull. Off. int. Épizoot. 57, 924.Google Scholar
Nathans, I., (1965). Vaccinatie van varkens tegen mond—en klauwzeer met geinactiveerd virus bevattende entstoffen. Thesis, University of Utrecht.Google Scholar
Reed, L. T., & Muench, H., (1938). A simple method of estimating fifty percent endpoints. Am. J. Hyg. 27, 493.Google Scholar
Skinner, H. H., (1951). Propagation of strains of foot-and-mouth disease virus in unweaned white mice. Proc. R. Soc. Med. 44, 1041.Google ScholarPubMed
Skinner, H. H., Henderson, W. M., & Brooksby, J. B., (1952). Use of unweaned white mice in foot-and-mouth disease research. Nature, Lond. 169, 79.CrossRefGoogle ScholarPubMed
Skinner, H. H., (1960). Some techniques for producing and studying attenuated strains of the virus of foot-and-mouth disease. Bull. Off. int. Épiznot. 53, 634.Google Scholar
Subak-Sharpe, H., (1961). The quantitative study of foot-and-mouth disease virus in unweaned mice. I. Studies of various factors affecting quantitative analysis. Arch. ges. Virusforsch. 11, 1.CrossRefGoogle Scholar