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Increased colonization potential of Campylobacter jejuni strain 81116 after passage through chickens and its implication on the rate of transmission within flocks

Published online by Cambridge University Press:  15 May 2009

S. A. Cawthraw ,
T. M. Wassenaar ,
R. Ayling  and
D. G. Newell
S. A. Cawthraw
Affiliation:
Applied and Molecular Immunology Unit, Central Veterinary Laboratory (Weybridge), Addlestone, Surrey KT15 3NB, UK
T. M. Wassenaar
Affiliation:
Department of Bacteriology, Institute of Immunology and Infectious Diseases, University of Utrecht, 3508 TD Utrecht, The Netherlands
R. Ayling
Affiliation:
Applied and Molecular Immunology Unit, Central Veterinary Laboratory (Weybridge), Addlestone, Surrey KT15 3NB, UK
D. G. Newell*
Affiliation:
Applied and Molecular Immunology Unit, Central Veterinary Laboratory (Weybridge), Addlestone, Surrey KT15 3NB, UK
*
† Author for correspondence.
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Summary

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The mechanisms by which Campylobacter jejuni rapidly spreads through large broiler flocks are unknown. Recent evidence suggests that the minimum infective oral dose for 100% caecal colonization is about 104 cfu, which, with such a ‘fragile’ organism, may limit transmissibility. However, the colonization potential of laboratory-adapted strains may be anomalous compared with fresh isolates or those passaged in vivo. The colonization potential of two chicken and one human C. jejuni isolates, which were minimally passaged, have been investigated using a quantitative model of chicken colonization. There was no detectable difference between these strains but all were more efficient colonizers than a laboratory-adapted strain 81116. In addition, the colonization potential of C. jejuni strain 81116 following a passage in vivo was also determined. The results indicated this increased C. 10000 fold following a single passage in vivo, such that a dose of only 40 cfu caused maximal colonization. Enhanced colonization potential may, therefore, account for the rapid rate of transmission within large flocks.

Type
Research Article
Information
Epidemiology & Infection , Volume 117 , Issue 1 , August 1996 , pp. 213 - 215
Copyright
Copyright © Cambridge University Press 1996

References

1.Advisory Committee on the Microbiological Safety of Food. Interim report on campylobacter. London: HMSO, 1993.Google Scholar
2.Humphrey, TJ, Henley, A, Lanning, DG. The colonization of broiler chickens with Campylobacter jejuni: some epidemiological investigations. Epidemiol Infect 1993; 110: 601–7.CrossRefGoogle ScholarPubMed
3.Evans, SJ. Introduction and spread of thermophilic campylobacters in broiler flocks. Vet Rec 1992; 131: 574–6.Google ScholarPubMed
4.Stern, NJ. Reservoirs for Campylobacter jejuni and approaches for intervention in poultry. In: Nachamkin, I, Blaser, MJ, Tomkins, LS, eds. Campylobacter jejuni, current status and future trends. Washington: ASM, 1992: 49–60.Google Scholar
5.Wassenaar, TM, van der Zeijst, BAM, Ayling, R, Newell, DG. Colonization of chicks by motility mutants of Campylobacter jejuni demonstrates the importance of flagellin A expression. J. Gen Microbiol 1993; 139: 1171–5.CrossRefGoogle ScholarPubMed
6.Sang, FC, Shane, SM, Yogasundram, K, Hagsted, HV, Kearnley, MT. Enhancement of Campylobacter jejuni virulence by serial passage in chicks. Avian Dis 1989; 33: 425–30.CrossRefGoogle ScholarPubMed
7.Palmer, SR, Gully, PR, White, JM, et al. Water-borne outbreak of Campylobacter enteritis. Lancet 1983; i: 287–90.CrossRefGoogle Scholar