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In vitro method for safety testing of foot-and-mouth disease vaccines

Published online by Cambridge University Press:  15 May 2009

E. C. Anderson ,
P. B. Capstick ,
G. N. Mowat  and
F. B. Leech
E. C. Anderson
Affiliation:
The Animal Virus Research Institute, Pirbright, Surrey
P. B. Capstick
Affiliation:
The Animal Virus Research Institute, Pirbright, Surrey
G. N. Mowat
Affiliation:
The Animal Virus Research Institute, Pirbright, Surrey
F. B. Leech
Affiliation:
Rothamsted Experimental Station, Harpenden, Herts.
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Summary

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The susceptibility of the tissue culture system to small amounts of residual live virus was not influenced by the inactivated antigen present. The depth of inoculum over the cell sheet did not affect results. Negative cultures frequently gave positive first (but not second or later) sub-cultures.

Baby hamster kidney cells were always more sensitive than cattle tongues to infection with any of the strains used.

Confidence in the safety test depends on the number of vaccination doses used; the tissue culture test can be made much more reliable than the cattle test because it is not limited to the 15 ml. of inoculum that restricts the cattle test.

Type
Research Article
Information
Journal of Hygiene , Volume 68 , Issue 2 , June 1970 , pp. 159 - 172
Copyright
Copyright © Cambridge University Press 1970

References

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