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Hybrids derived from the viruses of variola major and cowpox

Published online by Cambridge University Press:  15 May 2009

H. S. Bedson  and
K. R. Dumbell
H. S. Bedson
Affiliation:
Department of Bacteriology, University of Liverpool
K. R. Dumbell
Affiliation:
Department of Bacteriology, University of Liverpool
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The characterization of 16 clones of virus derived from mixed infections with variola major and cowpox has been described. This work has involved the description of the plaques produced by variola major in chick embryo monolayers, and a more detailed study of the effects of raised temperature on pock production by pox viruses on the C.A.M.

Two of the variola major—cowpox clones were found to correspond to cowpox virus, while the other 14 shared various combinations of parental characters. Among them there were 10 distinct new types of virus. Evidence of the stability of these viruses has been presented.

Analysis of the combination of characters encountered among the hybrids has given good evidence that all the eight marker characters used segregate independently. A particular instance of this was the character of chick embryo virulence which, among the hybrid viruses, was shown to be unrelated either to ceiling temperature or speed of plaque formation.

The significance of these findings in relation to the general problem of virulence has been briefly discussed. The possibility that vaccinia may have originated as a hybrid of variola and cowpox has lso been considered.

Type
Research Article
Information
Journal of Hygiene , Volume 62 , Issue 2 , June 1964 , pp. 147 - 158
Copyright
Copyright © Cambridge University Press 1964

References

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