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The HI test modified by ether treatment in the sero-epidemiological surveillance of influenza B

Published online by Cambridge University Press:  19 October 2009

R. Pyhälä ,
M. Kleemola  and
R. Visakorpi
R. Pyhälä
Affiliation:
National Public Health Institute, Mannerheimintie 166, SF–00280 Helsinki 28, Finland
M. Kleemola
Affiliation:
National Public Health Institute, Mannerheimintie 166, SF–00280 Helsinki 28, Finland
R. Visakorpi
Affiliation:
National Public Health Institute, Mannerheimintie 166, SF–00280 Helsinki 28, Finland
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Summary

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Ether-treated influenza B haemagglutination inhibition (HI) antigen was used in a study of serum collections from three different epidemic seasons.

For diagnostic purposes, ether treatment increased the efficacy of the HI test by about 50 % over the conventional HI technique, raising it to the same level of sensitivity as the complement fixation (CF) test. The treatment reduced the specificity of the HI test, but its reliability in the diagnosis of influenza B infections was only slightly diminished. With regard to evaluation of the immune status of a given population, an HI test using ether-treated antigen from the epidemic influenza B strain seems to give more relevant information about the antibody level associated with protection than a conventional HI test using untreated virus antigen.

Type
Research Article
Information
Journal of Hygiene , Volume 94 , Issue 3 , June 1985 , pp. 341 - 348
Copyright
Copyright © Cambridge University Press 1985

References

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