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Genetic diversity and clonal relationships of Acinetobacter baumannii strains isolated in a neonatal ward: epidemiological investigations by allozyme, whole-cell protein and antibiotic resistance analysis

Published online by Cambridge University Press:  15 May 2009

V. Thurm*
Affiliation:
Bundesgesundheitsamt, Robert von Ostertag-Institut, Bereich Wernigerode
E. Ritter
Affiliation:
Institut für Hygiene und Laboratoriumsmedizin, Städt. Krankenanstalten Krefeld, Germany
*
*Dr V. Thurm, Bundesgesundheitsamt, Robert von Ostertag-Institut. Bereich Wernigerode, Burgstr. 37, D-38855 Wernigerode, Germany.
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Summary

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Sixty-five strains of Acinetobacter baumannii which had been isolated from patients and the indoor environment of a neonatal intensive care unit and, for comparative purposes, isolates from three other wards, were examined by means of electrotyping and analysis of whole-cell protein and antibiotic resistance patterns. Fourteen different electrotypes were determined. The predominant type, a multiply resistant acinetobacter clone, persisted in the neonatal ward over several months. The results underline the usefulness of electrophoretic subtyping, in particular by means of allozyme pattern and as a supplement to whole-cell protein pattern analysis, in epidemiological investigations into the routes of transmission of nosocomial A. baumannii infections.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1993

References

1.Bergogone-Berezin, E, Joly-Guillou, ML. Hospital infection with Acinetobacter spp.: an increasing problem. J Hosp Infect 1991; 18 (Suppl. A): 250–5.CrossRefGoogle Scholar
2.Beck-Sague, CM, Carvis, WR, Brook, JH, et al. Epidemic bacteremia due to Acinetobacter baumannii in five intensive care units. Am J Epidemiol 1990; 132: 723–3.CrossRefGoogle ScholarPubMed
3.Traub, WH. Acinetobacter baumannii serotyping for delineation of outbreaks of nosocomial cross infections. J Clin Microbiol 1989; 27: 2713–6.CrossRefGoogle Scholar
4.Bouvet, PJM, Grimont, PAD. Identification and biotyping of clinical isolates of Acinetobacter. Ann Microbiol (Inst Pasteur) 1987; 138: 569–78.CrossRefGoogle ScholarPubMed
5.Vieu, JF. Bacteriophages et lysotypie de Acinetobacter. Ann Microbiol (Inst Pasteur) 1979: 130A: 405–6.Google Scholar
6.Traub, WH, Spohr, M. Antimicrobial drug susceptibility of clinical isolates of Acinetobacter species (A. baumannii, A. haemolyticus, genospecies 3, and genospecies 6). Antimicrob Agents Chemother 1989; 33; 1617–9.CrossRefGoogle ScholarPubMed
7.Dijkshoorn, L, Michel, MF, Degener, JE. Cell envelope protein profiles of Acinetobacter calcoaceticus strains isolated in hospitals. J Med Microbiol 1987; 23: 313–9.CrossRefGoogle ScholarPubMed
8.Ritter, E, Thurm, V, Becker-Boost, E, Thomas, P, Finger, H, Wirsing von König, CH. Epidemisches Vorkommen multiresistenter Acinetobacter baumannii-Stämme auf einer neonatologischen Intensivstation. Zbl Hyg 1993; 193: 461–70.Google Scholar
9.Bouvet, PJM, Grimont, PAD. Taxonomy of the genus Acinetobacter with the recognition of A. baumannii sp. nov., A. haemolyticus sp. nov., A. johnsonii sp. nov., and A. junii sp. nov. and emended descriptions of A. calcoaceticus and A. lwoffii. Int J System Bacteriol 1986; 36: 228–40.CrossRefGoogle Scholar
10.Selander, RK, Caugant, DA, Ochman, H, Musser, JM, Gilmour, MN, Whittam, TS. Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl Environ Microbiol 1986; 51: 873–84.CrossRefGoogle ScholarPubMed
11.Thurm, V. Anwendung der Agarosegelelektrophorese ausgewählter Alloloenzyme für die Elektrotypisierung klinisch bedeutsamer Bakterien zur epidemiologischen Analyse von Hospital- u.a. Infektionen. Zeitschr Laboratoriumsmedizin 1991; 10: 496500.CrossRefGoogle Scholar
12.Costas, M. Numerical analysis of sodium dodecylsulfate-polyacrylamide gel electrophoretic protein patterns for classification, identification and typing of medically important bacteria. Electrophoresis 1990; 11: 382–91.CrossRefGoogle Scholar
13. DIN 58940 (1992) Teil 3: Medizinische Mikrobiologie; Methoden zur Empfindlichkeitsprüfung von bakteriellen Krankheitserregern (auβer Mykobakterien) gegen Chemotherapeutika; Agar-Diffusionstest.Google Scholar
14. DIN 58940 (1992) Teil 6: Medizinische Mikrobiologie; Methoden zur Empfindlichkeitsprüfung von bakteriellen Krankheitserregern (auβer Mykobakterien) gegen Chemotherapeutika; Bestimmung der minimalen Hemmkonzentration nach der Agar-Dilutionsmethode.Google Scholar
15.Dijkshoorn, L, van Ooyen, S, Hop, WCJ, Theuns, M, Michel, MF. Comparison of clinical Acinetobacter strains using a carbon source growth assay. Epidemiol Infect 1990; 104: 443–53.CrossRefGoogle ScholarPubMed
16.Bouvet, PJM, Jeanjean, S, Vieu, JF, Dijkshoorn, L. Species, biotype, and bacteriophage type determinations compared with cell envelope protein profiles for typing Acinetobacter strains. J Clin Microbiol 1990; 28: 170–6.CrossRefGoogle ScholarPubMed
17.Selander, RK. Genetic relationships and clonal structure of strains of E. coli causing neonatal septicemia and meningitis. Infect Immun 1986; 52: 213–8.CrossRefGoogle Scholar