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Further characterization of 41 isolates of adenovirus types 19/37 by serum neutralization and DNA restriction enzyme analysis

Published online by Cambridge University Press:  19 October 2009

Zong-Da Meng
Affiliation:
Department of Virology, Hebei Provincial Antiepidemic and Health Station, Baoding, China
Margery L. Kennett
Affiliation:
Virology Department, Fairfield Hospital for Communicable Diseases, Fairfield, 3078 Australia
Suzanne M. Rodger
Affiliation:
Virology Department, Fairfield Hospital for Communicable Diseases, Fairfield, 3078 Australia
Kaye E. Dickson
Affiliation:
Virology Department, Fairfield Hospital for Communicable Diseases, Fairfield, 3078 Australia
Bruce N. Anderson
Affiliation:
Virology Department, Fairfield Hospital for Communicable Diseases, Fairfield, 3078 Australia
Ian D. Gust
Affiliation:
Virology Department, Fairfield Hospital for Communicable Diseases, Fairfield, 3078 Australia
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Summary

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Forty-one strains of adenovirus type 19/37 (Ad19/37) mainly isolatedI from patients with keratoconjunetivitis or conjunctive between 1974 and 1984 were re-evaluated by serum neutralization (SN), haemagglutination inhibition (HI) and DNA restriction analysis. Of 19 isolates which were neutralized to high titre by antiserum prepared against prototype Ad19, 5 showed cross-reactivity with 32–64 units of Ad37 antiserum, while of 22 strains neutralizedto high titre by Ad37 antiserum, 3 showed eross-reaetivity with 32 units of Ad19 antiserum By DNA restriction analysis, all Ad19 isolates were identieal to each other and to Ad19A virus. Using endonuclease Bgl 1, three variants were observed among the Ad37 isolates.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1986

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