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Frequency of failure to isolate Shigella spp. by the direct plating technique and improvement of isolation by enrichment in selenite broth

Published online by Cambridge University Press:  26 November 2001

K. MIYAGI ,
Y. TAKEGAKI ,
T. NAKANO ,
Y. NAKANO ,
T. HONDA  and
K. SANO
K. MIYAGI
Affiliation:
Department of Microbiology, Osaka Medical College, Takatsuki-shi, Osaka 569-8686, Japan Osaka Quarantine Station, Osaka-shi, Osaka 552-0021, Japan
Y. TAKEGAKI
Affiliation:
Nagoya Airport Quarantine Station Branch Office, Toyoyama-cho, Nishikasugai-gun, Aichi 480-0202, Japan
T. NAKANO
Affiliation:
Department of Microbiology, Osaka Medical College, Takatsuki-shi, Osaka 569-8686, Japan Department of Medical Technology, Kobe-Tokiwa College, Kobe-shi, Hyogo 653-0838, Japan
Y. NAKANO
Affiliation:
Osaka Quarantine Station, Osaka-shi, Osaka 552-0021, Japan
T. HONDA
Affiliation:
Research Institute for Microbial Diseases, Osaka University, Suita-shi, Osaka 565-0871, Japan
K. SANO
Affiliation:
Department of Microbiology, Osaka Medical College, Takatsuki-shi, Osaka 569-8686, Japan Department of Medical Technology, Kobe-Tokiwa College, Kobe-shi, Hyogo 653-0838, Japan
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Abstract

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In order to clarify the failure to isolate Shigella spp. by direct plating, we compared frequencies of Shigella spp. isolation by direct plating and by plating after enrichment in selenite broth. A total 67 strains were isolated in this study. The strains of 38 (56·7%) were isolated only by direct plating, and 25 (37·3%) strains were isolated by both direct plating and after enrichment. Four strains (6·0%) were isolated after enrichment but not by direct plating. Since 6% of isolated Shigella spp. were not isolated by direct plating, we recommend that direct plating and additional isolations from selenite broth should be performed. The significance concerning reduction of concentration of sodium selenite in enrichment broth is discussed.

Type
Short Paper
Information
Epidemiology & Infection , Volume 127 , Issue 2 , October 2001 , pp. 375 - 379
Copyright
2001 Cambridge University Press