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Felton Antibody: its Distribution and Purity as determined by Salting Out Methods

Published online by Cambridge University Press:  15 May 2009

Annie M. Brown
Annie M. Brown
Affiliation:
From the Wellcome Physiological Research Laboratories, Beckenham, Kent.
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The results obtained show:

1. The limits of precipitation of Felton antibody with solid ammonium sulphate in several sera; the effect on these limits of the addition of purified Felton antibody and the removal of tricresol from the serum.

2. That within these limits of precipitation small fractions can be obtained which differ from each other in their ratio of antibody to protein.

3. That the purity of such fractions throughout a given serum may be reduced by the addition of purified Pneumococcus antibody to the serum, and apparently increased by the addition of tricresol.

4. That the distribution of antibody in serum taken at intervals from a horse during immunisation changes progressively.

5. Differences in the distribution of the two antibodies in serum from a horse immunised to both Pneumococcus type I and diphtheria toxin.

Type
Research Article
Information
Journal of Hygiene , Volume 33 , Issue 2 , April 1933 , pp. 252 - 258
Copyright
Copyright © Cambridge University Press 1933

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