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Experimental phycomycosis in mice; examination of the role of acquired immunity in resistance to Absidia ramosa

Published online by Cambridge University Press:  15 May 2009

M. J. Corbel  and
Susan M. Eades
M. J. Corbel
Affiliation:
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, Weybridge, Surrey
Susan M. Eades
Affiliation:
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, Weybridge, Surrey
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Attempts were made to stimulate acquired immunity to experimental Absidia ramosa infection in mice. Unprotected animals inoculated with large doses of A. ramosa spores frequently developed acute phycomycosis of the central nervous system. Mice previously exposed to sub-lethal doses of spores showed a high resistance to subsequent challenge with A. ramosa. No consistent increase in resistance was observed in mice vaccinated with killed A. ramosa spores, hyphal walls, intracellular mycelial antigens or various combinations of these, with Freund's incomplete adjuvant.

Antibodies to soluble mycelial antigens were inconsistently present in the sera of mice vaccinated with sub-lethal doses of viable spores. They were generally present in the sera of animals vaccinated with mycelial extracts or hyphal walls but not killed spores.

Delayed hypersensitivity reactions to A. ramosa mycelial antigens could usually be elicited by intradermal tests in mice exposed to viable spores but irregularly in those vaccinated with non-viable preparations. Positive reactions were also frequently given by older mice not deliberately exposed to A. ramosa.

Although mice previously exposed to viable A. ramosa spores were highly resistant to intravenous or intracerebral challenge with this fungus, they were more likely to develop persistent local granulomata on subcutaneous injection of spores than were unvaccinated animals.

Type
Research Article
Information
Journal of Hygiene , Volume 77 , Issue 2 , October 1976 , pp. 221 - 233
Copyright
Copyright © Cambridge University Press 1976

References

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