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Effect of respiratory syncytial virus infection on binding of Neisseria meningitidis and Haemophilus influenzae type b to a human epithelial cell line (HEp-2)

Published online by Cambridge University Press:  19 October 2009

M. W. Raza ,
M. M. Ogilvie ,
C. C. Blackwell ,
J. Stewart ,
R. A. Elton  and
D. M. Weir
M. W. Raza
Affiliation:
Department of Medical Microbiology, University of Edinburgh Medical School, Teviot Place, Edinburgh EHS 9AG
M. M. Ogilvie
Affiliation:
Department of Medical Microbiology, University of Edinburgh Medical School, Teviot Place, Edinburgh EHS 9AG
C. C. Blackwell*
Affiliation:
Department of Medical Microbiology, University of Edinburgh Medical School, Teviot Place, Edinburgh EHS 9AG
J. Stewart
Affiliation:
Department of Medical Microbiology, University of Edinburgh Medical School, Teviot Place, Edinburgh EHS 9AG
R. A. Elton
Affiliation:
Medical Statistics Unit, Department of Public Health Sciences, University of Edinburgh Medical School, Teviot Place, Edinburgh EHS 9AG
*
*Corresponding author.
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It has been suggested that individuals might be more readily colonized with bacteria that cause meningitis through enhanced binding of the bacteria to virusinfected epithelial cells. As respiratory syncytial virus (RSV) affects infants and children in the age group also susceptible to bacterial meningitis, we tested the hypothesis that infection of HEp-2 cells by RSV might enhance binding of Neisseria meningitidis or Haemophilus influenzae type b (Hib). Attachment of fluorescein-labelled bacteria to HEp-2 cells was measured by flow cytometry, and RSV-infected cells bound significantly more meningococci (P < 0·001) and Hib (P < 0·01) than uninfected cells. Although the isolates expressed different antigenic characteristics (3 meningococci and 5 Hib), all showed a similar pattern of binding. The results are discussed with reference to the methods used for detection of bacterial binding and to interactions that might explain the increased binding to RSV-infected cells.

Type
Research Article
Information
Epidemiology & Infection , Volume 110 , Issue 2 , April 1993 , pp. 339 - 347
Copyright
Copyright © Cambridge University Press 1993

References

REFERENCES

1.Krasinski, K, Nelson, JD, Butler, S, Luby, JP, Kusmiesz, H. Possible association of mycoplasma and viral respiratory infections with bacterial meningitis. Am J. Epidem 1987; 125: 499–508.CrossRefGoogle ScholarPubMed
2.Ramírez-Ronda, CH, Fuxench-López, Z, Névarez, M. Increased pharyngeal bacterial colonization during viral illness. Arch Int Med 1981; 141: 1599–603.CrossRefGoogle ScholarPubMed
3.Stanford, BA, Davison, VE, Ramsay, MA. Slaphylococcus aureus adherence to influenza A virus infected and control cell cultures: evidence for multiple adhesions. Proc Soc Exp Biol Med 1986; 181: 104–11.CrossRefGoogle Scholar
4.Young, LS, La Force, FM, Head, JJ, Felley, JC, Bennet, JV. A simultaneous outbreak of meningococcal and influenza infection. New Eng J Med 1972; 287: 5–9.CrossRefGoogle Scholar
5.Gwaltney, JM, Sande, MA, Austrian, R, Hendley, JO. Spread of Slreptoeoecus pneumoniae in families. II. Relation of transfer of S. pneumoniae to incidence of colds and serum antibody. J Inf Dis 1975; 132: 62–8.CrossRefGoogle ScholarPubMed
6.Hietella, J, Uhari, M, Tuokko, H, Leinonen, M. Mixed bacterial and viral infections are common in children. Pediatric Infect Dis J 1989; 8: 683–6.CrossRefGoogle Scholar
7.Babiuk, LA, Lawman, MJP, Ohmann, HB. Viral-bacterial synergistic interaction in respiratory disease. Adv Vir Res 1988; 35: 219–49.CrossRefGoogle ScholarPubMed
8.Jakab, GJ. Immune impairment of alveolar macrophage phagocytosis during influenza virus pneumonia. Am Rev Resp Dis 1982; 126: 778–82.Google ScholarPubMed
9.Jakab, GJ. Mechanisms of virus-induced bacterial superinfections of the lung. Clin Chest Med 1981; 2: 59–66.CrossRefGoogle ScholarPubMed
10.Martin, RR. Couch, RB, Greenberg, SB, Cate , TR, Warr, GA. Effects of infection with influenza virus on the function of polymorphonuclear leucocytes. J Inf Dis 1981; 144: 279.CrossRefGoogle Scholar
11.Camner, P, Jarstrand, C, Philipson, K. Tracheobronchial clearance in patients with influenza. Am Rev Res Dis 1973; 108: 131–5.Google ScholarPubMed
12.Plotkowski, M, Puchelle, E, Beck, G, Jacquot, J, Hannoun, C. Adherence of type 1 Streptococcus pnexnnoniae to tracheal epithelium of mice infected with influenza A/PRS virus. Am Rev Resp Dis 1986; 134: 1040–4.CrossRefGoogle Scholar
13.Musher, DM, Fainstein, V. Adherence of Staphylococcus aureus to pharyngeal cells from normal subjects, smokers, staphylococeal carriers and patients with viral infections. In: Jeljaszewicz, J, ed. Staphylocoeei and staphyloeoeeal infections. New York: Gustav Fischer Verlag, Zbl Bak (Suppl 10), 1981: 1011–16.Google Scholar
14.Bakaletz, LO, Hoepf, TM, DeMaria, TF, Lim, DJ. The effect of antecedent influenza A virus infection on the adherence of Haemophilus injluenzae to chinchilla tracheal epithelium. Am J Otolaryngol 1988; 9: 127–34.CrossRefGoogle ScholarPubMed
15.Fainstein, V, Musher, DM. Cate, TR. Bacterial adherence to pharyngeal cells during viral infection. J Inf Dis 1980; 141: 172–6.CrossRefGoogle ScholarPubMed
16.Moore, PS, Heirholzer, J, DeWitt, W et al. Respiratory viruses and mycoplasma as co-factors for epidemic group A meningococcal meningitis. JAMA 1990; 264: 1271–5.CrossRefGoogle Scholar
17.Cartwright, KAV, Jones, DM, Smith, AJ, Stuart, JM, Kaczmarski, EB, Palmer, SR. Influenza A and meningococcal disease. Lancet 1991; 338: 554–7.CrossRefGoogle ScholarPubMed
18.Henderson, FW, Collier, AM, Clyde, WA Jr. Denny, FW. Respiratory syneytial virus infection, reinfection and immunity: a prospective longitudinal studv in young children. N Eng J Med 1979; 300: 530–4.CrossRefGoogle Scholar
19.Greenwood, BM. Selective primary health care: strategies for control of disease in the developing world. XIII. Acute bacterial meningitis. Rev Inf Dis 1984; 6: 374–89.CrossRefGoogle ScholarPubMed
20.Blackwell, CC. The role of ABO blood groups and secretor status in host defences. FEMS Microbiol Immunol 1989; 47: 341–50.CrossRefGoogle Scholar
21.Stephens, DS. Gonococcal and meningococcal pathogenesis as defined by human cell, cell culture and organ culture assays. Clin Microbiol Rev 1989; suppl 2: s1O4–11.CrossRefGoogle Scholar
22.Layh-Schmitt, G, Schmitt, S, Buchanan, TM. Interaction of non-pilate Neisseria gonorrhoeae strain 7122 and protein IA with an epithelial cell monolayer. Int J Med Microbiol 1989; 272: 158–70.Google Scholar
23.Ogilvie, MM, Vathenen, AS, Radford, M, Codd, J, Key, S. Maternal antibody and respiratory syncytial virus infection in infancy. J Med Virol 1981; 7: 263–71.CrossRefGoogle ScholarPubMed
24.Young, H. Cultural diagnosis of gonorrhoea with modified New York City (MXYC) medium. Br J Vener Dis 1978; 54: 36–40.Google ScholarPubMed
25.Rabat, A. Studies on the role of blood group antigens in susceptibility to meningococcal diseases. PhD thesis. 1990. Edinburgh University. UK: 83–4.Google Scholar
26.St Geme, JW, Falkow, S. Haemophilus injluenzae adheres to and enters cultured human epithelial cells. Infect Immun 1990; 58: 4030–44.CrossRefGoogle ScholarPubMed
27.Nichol, KP, Cherry, JD. Bacterial-viral interrelations in respiratory infections of children. N Eng J Med 1967; 277: 667–72.CrossRefGoogle ScholarPubMed
28.Stephen, DS, Hoffman, LH, McGee, ZA. Interaction of Xeisseria meningitidis with human naso-pharyngeal mucosa: attachment and entry into columnar epithelial cells. J Inf Dis 1983; 148: 369–76.CrossRefGoogle Scholar
29.Parry, JE, Shirodria, PV. Pringle, CR. Pneumoviruses: the cell surface of lytically and persistently infected cells. J Gen Virol 1979: 44: 479–91.CrossRefGoogle ScholarPubMed