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Direct complement fixation test with avian infectious bronchitis virus in chickens

Published online by Cambridge University Press:  15 May 2009

P. K. Uppal
P. K. Uppal
Affiliation:
Department of Animal Pathology, University of Cambridge, Cambridge
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The direct complement fixation test was performed to follow the antibody response in chickens infected with avian infectious bronchitis virus. Concentrated allantoic fluid (4 units) was used as an antigen and allowed to react with serially diluted antiserum in the presence of two complete units of guinea-pig complement for 3 hr. at 4°C. and ½ hr. at 37°C. before the addition of sensitized cells. Serum was unheated and used either fresh or within one month of storage at −30°C. Individual birds showed a rise and fall of complement-fixing antibody both after primary and secondary inoculations. The complement-fixing antibody was detected as early as the seventh day after primary inoculation. The highest complement fixation titre (1/32 to 1/64) was recorded from 14 to 21 days after inoculation with a subsequent gradual decline.

The results of the direct complement fixation tests have been correlated with the serum neutralization test. The neutralizing antibodies usually appeared by the 14th day but were not detected at a significant titre until the 21st day after primary inoculation. Serum neutralizing antibodies were still present at high titres even after 7 weeks of infection but the complement-fixing antibodies had disappeared by that time.

Type
Research Article
Information
Journal of Hygiene , Volume 68 , Issue 2 , June 1970 , pp. 211 - 219
Copyright
Copyright © Cambridge University Press 1970

References

REFERENCES

Brumfield, H. P., Benjamin, S. & Pomeroy, B. S. (1959). Test based on normal serum component implementing fixation of turkey antiserum. Proceedings of the Society for Experimental Biology and Medicine 102, 278.CrossRefGoogle ScholarPubMed
Brumfield, H. P. & Pomeroy, B. S. (1957). Direct complement fixation by turkey and chicken sera in viral system. Proceedings of the Society for Experimental Biology and Medicine 94, 146.CrossRefGoogle Scholar
Bukantz, S. C., Rein, C. R. & Kent, J. F. (1946). Studies in complement fixation. 2. Preservation of sheep's blood in citrate dextrose mixtures (modified). Journal of Laboratory and Clinical Medicine 31, 394.Google Scholar
Bushnell, L. D. & Hudson, C. B. (1927). Complement fixation and agglutination tests for Salmonella pullorum infection. Journal of Infectious Diseases 41, 388.CrossRefGoogle Scholar
Harada, K. & Matumato, M. (1962). Antigen relationship between mammalian and avian poxes as revealed by direct complement fixation reaction. Japanese Journal of Experimental Medicine 32, 369.Google Scholar
Kato, K. & Horiuchi, T. (1965). Isolation of sparrow pox and its serological properties. National Institute of Animal Health Quarterly 5, 130.Google Scholar
Mayer, M. M., Osler, A. G., Bier, O. G. & Heidelberger, M. (1946). Activating effect of magnesium and other cations on the hemolytic function of complement. Journal of Experimental Medicine 84, 535.Google Scholar
Nitzschke, E. (1954). Über den Antikörpernachweis mit der Komplementbindungs-und Komplementbindungs-Hemmungs-Reaktion bei geflugelpestinfizierten Huhnern. Zentralblatt für Veterinärmedizin 1, 565.CrossRefGoogle Scholar
Nitzschke, E. (1956). Untersuchungen über die Komplementbindungs-und Komplement-bindungs-Hemmungs-Reaktion mit Huhner-Immunisieren gegen die Viren der atypischen und der klassischen Geflugelpest sowie der Schweine-Influenza. Zentralblatt für Veterinärmedizin 3, 75.CrossRefGoogle Scholar
Orlans, E., Rose, M. E. & Clapp, K. H. (1962 a). Fowl antibody. V. The interactions of fresh and heated fowl serum and of guinea-pig complement measured by the lysis of sensitized red cells. Immunology 5, 649.Google ScholarPubMed
Orlans, E., Rose, M. E. & Clapp, K. H. (1962 b). Fowl antibody. VI. The fixation of complement by antibody to soluble antigens. The effect of fresh normal fowl serum on the precipitation reaction. Immunology 5, 656.Google ScholarPubMed
Rice, C. E. (1947). Atypical behaviour of certain avian antisera in complement fixation tests. Canadian Journal of Comparative Medicine 11, 236.Google Scholar
Rice, C. E. (1948). Studies in pullorum disease. XII. Technique of the indirect complement fixation test for activity with salmonella pullorum antigen. Canadian Journal of Comparative Medicine 12, 130.Google Scholar
Rice, C. E., Magwood, S. E. & Annau, E. (1960). A modified direct complement fixation test for the detection of antibodies for salmonella antigen in turkey sera. Canadian Veterinary Journal 1, 132.Google ScholarPubMed
Sonnenschein, C. (1930). Komplementkonservierung durch Natriumazetat und Borsaure. Zeitschrift für Immunitätsforschung und experimentelle Therapie 67, 512.Google Scholar
Tsubahara, H., Kataoka, T. & Kato, K. (1961). Complement fixation in fowl pox. III. Direct complement fixation with fowl serum. Bulletin of the National Institute of Animal Health. No. 41, 1.Google Scholar