Hostname: page-component-848d4c4894-xfwgj Total loading time: 0 Render date: 2024-06-30T08:26:05.753Z Has data issue: false hasContentIssue false
  • English
  • Français

The development and assessment of a bacteriocin typing method for Klebsiella

Published online by Cambridge University Press:  15 May 2009

A. S. Edmondson  and
E. Mary Cooke
A. S. Edmondson
Affiliation:
Department of Microbiology, School of Medicine, Leeds LS2 9NL
E. Mary Cooke
Affiliation:
Department of Microbiology, School of Medicine, Leeds LS2 9NL
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Klebsiellas are generally typed by the method of capsular serotyping but, although this is a reliable method, it is time consuming, requires the production of a large number of antisera and is not generally available. For this reason another method for typing klebsiellas was sought.

A bacteriocin typing method involving mitomycin C induction was developed and the cultural conditions giving optimum klebecin production and the best methods of testing the sensitivity of the organisms to klebecins were determined.

Of 190 klebsiella strains screened for bacteriocinogeny, only 68 (35·8%) produced klebecin and after calculation of similarity values by computer analysis, a typing set of 15 producers was selected. This typing set allowed over 96% of klebsiella strains to be typed and tests of the reproducibility of the method and the variability of typing patterns in natural populations of klebsiella indicated that results of acceptable accuracy could be obtained, while retaining good discrimination if two or more differences were required between patterns before they were regarded as distinct.

A complete set of capsular antisera were prepared, enabling the results obtained from klebecin typing to be compared with those from serotyping. There was generally close agreement between the results from the two typing methods and greater discrimination was obtained between similar strains when the two methods were combined. Klebecin typing and serotyping revealed relationships between strains from five outbreaks of infection, and strains of the same serotype from different hospitals could frequently be distinguished by their klebecin typing patterns.

Type
Research Article
Information
Journal of Hygiene , Volume 82 , Issue 2 , April 1979 , pp. 207 - 223
Copyright
Copyright © Cambridge University Press 1979

References

REFERENCES

Abbott, J. D. & Shannon, R. (1958). A method for typing Shigella sonnei, using colicine production as a marker. Journal of Clinical Pathology 11, 71.CrossRefGoogle ScholarPubMed
Al-Jumaili, I. J. (1975). An evaluation of two methods of bacteriocine typing of organisms of the genus Proteus. Journal of Clinical Pathology 28, 788.CrossRefGoogle ScholarPubMed
Anderhub, B., Pitt, T. L., Erdman, Y. T. & Willcox, W. R. (1977). A comparison of typing methods for Serratia marcescens. Journal of Hygiene 79, 89.CrossRefGoogle ScholarPubMed
Buffenmeyer, C. L., Rycheck, R. R. & Yee, R. B. (1976). Bacteriocin (klebocin) sensitivity typing of Klebsiella. Journal of Clinical Microbiology 4, 239.CrossRefGoogle Scholar
Casewell, M. W. (1975). Titres and cross-reactions of commercial antisera for the capsular typing of Klebsiella species. Journal of Clinical Pathology 28, 33.CrossRefGoogle ScholarPubMed
Cowan, S. T. & Steel, K. J. (1974). Manual for the Identification of Medical Bacteria, 2nd ed., revised by Cowan, S. T.. Cambridge University Press.Google Scholar
Cradook-Watson, J. E. (1965). The production of bacteriocines by Proteus species. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene, Abt. 1, Orig. 196, 385.Google Scholar
Curie, K., Speller, D. C. E., Simpson, R. A., Stephens, M. & Cooke, D. I. (1978). A hospital epidemic caused by a gentamicin-resistant Klebsiella aerogenes. Journal of Hygiene 80, 115.CrossRefGoogle ScholarPubMed
Dans, P. E., Barrett, F. F., Casey, J. I. & Finland, M. (1970). Klebsiella–Enterobacter at Boston City Hospital, 1967. Archives of Internal Medicine 125, 94.CrossRefGoogle ScholarPubMed
Donovan, T. J. (1966). A Klebsiella screening medium. Journal of Medical Laboratory Technology 23, 194.Google ScholarPubMed
Duncan, I. B. R. & Rennie, R. P. (1974). Klebsiella in hospital-acquired infections. Lancet ii, 157.CrossRefGoogle Scholar
Farmer, J. J. III (1970). Mnemonic for reporting bacteriocin and bacteriophage types. Lancet ii, 96.CrossRefGoogle Scholar
Farmer, J. J. III (1972a). Epidemiological differentiation of Serratia marcescens: typing by bacteriocin production. Applied Microbiology 23, 218.CrossRefGoogle ScholarPubMed
Farmer, J. J. III (1972b). Epidemiological differentiation of Serratia marcescens: typing by bacteriocin sensitivity. Applied Microbiology 23, 226.CrossRefGoogle ScholarPubMed
Farmer, J. J. III & Herman, L. G. (1969). Epidemiological fingerprinting of Pseudomonas aeruginosa by the production of and sensitivity to pyocin and bacteriophage. Applied Microbiology 18, 760.CrossRefGoogle ScholarPubMed
George, R. H. (1974). Comparison of different media for bactoriocine typing of Proteus mirabilis. Journal of Clinical Pathology 28, 25.CrossRefGoogle Scholar
Hable, K. A., Matsen, J. M., Wheeler, D. J., Hunt, C. E. & Quie, P. G. (1972). Kiebsiella type 33 septicemia in an intensive care unit. Journal of Pediatrics 80, 920.CrossRefGoogle Scholar
Hall, F. A. (1971). Bacteriocine typing of Klebsiella spp. Journal of Clinical Pathology 24, 712.CrossRefGoogle ScholarPubMed
Hayes, P. R. (1975). Ph.D. thesis, Leeds University.Google Scholar
Hill, H. R., Hunt, C. E. & Matsen, J. M. (1974). Nosocomial colonization with Klebsiella, type 26, in a neonatal intensive care unit associated with an outbreak of sepsis, meningitis, and necrotizing enterocolitis. Journal of Pediatrics 85, 415.CrossRefGoogle Scholar
Kauffmann, F. (1949). On the serology of the Klebsiella group. Acta pathologica et microbiologica scandinavica 26, 381.CrossRefGoogle Scholar
Lancet (leading article) (1971). Epidemiology of klebsiella infections. Lancet ii, 416.Google Scholar
Mayr-Harting, A., Hedges, A. J. & Berkeley, R. C. W. (1972). Methods for studying bacteriocins. Methods in Microbiology 7 A, 315.CrossRefGoogle Scholar
Ørskov, I. (1955). Serological investigations in the Klebsiella group. 1. New capsule types. Acta pathologica et microbiologica scandinavica 36, 449.CrossRefGoogle Scholar
Ørskov, I. (1957). Biochemical types in the Klebsiella group. Acta pathologica et microbiologica scandinavica 40, 155.CrossRefGoogle ScholarPubMed
Pau, W. S. & Terry, C. S. (1976). The effect of medium composition upon the production of pyocin. Journal of Applied Bacteriology 41, 369.CrossRefGoogle ScholarPubMed
Price, D. J. E. & Sleigh, J. D. (1970). Control of infection due to Klebsiella aerogenes in a neurosurgical unit by withdrawal of all antibiotics. Lancet ii, 1213.CrossRefGoogle Scholar
Rampling, A., Whitby, J. L. & Wildy, P. (1975). Pyocin-sensitivity testing as a method of typing Pseudomonas aeruginosa: use of ‘phage-free’ preparations of pyocin. Journal of Medical Microbiology 8, 531.CrossRefGoogle ScholarPubMed
Riser, E., Noone, P. & Bonnet, M.-L. (1976). A new serotyping method for Klebsiella species; evaluation of the technique. Journal of Clinical Pathology 29, 305.CrossRefGoogle ScholarPubMed
Riser, E., Noone, P. & Poulton, T. A. (1976). A new serotyping method for Klebsiella species: development of the technique. Journal of Clinical Pathology 29, 296.CrossRefGoogle ScholarPubMed
Senior, B. W. (1977). Typing of Proteus strains by proticine production and sensitivity. Journal of Medical Microbiology 10, 7.CrossRefGoogle ScholarPubMed
Slopek, S. & Maresz-Babczyszyn, J. (1967). A working scheme for typing Klebsiella bacilli by means of pneumocins. Archivum Immunologiae et Therapiae experimentalis 15, 525.Google ScholarPubMed
Slopek, S., Przondo-Hessek, A., Milch, H. & Deak, S. (1967). A working scheme for bacteriophage typing of Klebsiella bacilli. Archivum Immunologiae et Therapiae experimentalis 15, 589.Google ScholarPubMed
Sneath, P. H. A. (1957). The application of computers to taxonomy. Journal of General Microbiology 17, 201.Google ScholarPubMed
Steinhauer, B. W., Eickhoff, T. C., Kislak, J. W. & Finland, M. (1966). The Klebsiella–Enterobacter–Serratia division. Clinical and epidemiologic characteristics. Annals of Internal Medicine 65, 1180.CrossRefGoogle ScholarPubMed
Traub, W. A., Raymond, E. A. & Startsman, T. S. (1971). Bacteriocin (marcescin) typing of clinical isolates of Serratia marcescens. Applied Microbiology 21, 837.CrossRefGoogle ScholarPubMed
Williams, R. E. O. & Rippon, J. E. (1952). Bacteriophage typing of Staphylococcus aureus. Journal of Hygiene 50, 320.CrossRefGoogle ScholarPubMed