Hostname: page-component-84b7d79bbc-5lx2p Total loading time: 0 Render date: 2024-07-30T17:36:31.941Z Has data issue: false hasContentIssue false
  • English
  • Français

The detection of peste des petits ruminants (PPR) virus antigen by agar gel precipitation test and counter-immunoelectrophoresis

Published online by Cambridge University Press:  19 October 2009

T. U. Obi  and
D. Patrick
T. U. Obi
Affiliation:
Department of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria
D. Patrick
Affiliation:
National Veterinary Research Institute, Vom, Plateau State, Nigeria
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The detectability of peste des petits ruminants (PPR) viral antigen in both ante-mortem secretions and necropsy samples from experimentally infected goats was investigated by both the agar gel precipitation test (AGPT) and counterimmunoelectrophoresis (CIE). Viral antigen was detected from 42·6% of the samples tested by the AGPT and 80–3% by CIE. The detection of viral antigen in a high proportion of the ocular and nasal secretions as well as the faeces and buccal scrapings, particularly from those collected within seven days of the onset of fever, by both techniques, would seem to obviate the need for lymph node biopsies or post-mortem samples in order to make a diagnosis of PPRV infection.

Type
Research Article
Information
Journal of Hygiene , Volume 93 , Issue 3 , December 1984 , pp. 579 - 586
Copyright
Copyright © Cambridge University Press 1984

References

Ali, B. E. H. & Lees, C. E. (1979). The application of immunoelectroprecipitation in the diagnosis of rinderpest. Bulletin of Animal Health and Production in Africa 27, 1–6.Google Scholar
Appel, M. J. G., Gibbs, E. P. J., Martin, S. J., Meulen, V. T.Rima, B. T., Stephenson, J. R. & Taylor, W. F. (1981). Morbillivirus diseases of animals and man. In Comparative Diagnosis of Viral Disease, vol. 4, pp. 276–291. New York: Academic Press.Google Scholar
Brown, R. D. & Scott, G. R. (1960). Diagnosis of rinderpest by lymph node biopsy. Veterinary Record 72, 1055–1056.Google Scholar
Daubney, R. (1928). Observations on rinderpest. Journal of Comparative Pathology 41, 228–248.CrossRefGoogle Scholar
Forman, A. J., Rowe, L. W. & Taylor, W. P. (1983). The detection of rinderpest antigen by agar gel diffusion and counterimmunoelectrophoresis. Tropical Animal Health and Production 15, 83–85.CrossRefGoogle ScholarPubMed
Gibbs, E. P. J., Taylor, W. P., Lawman, M. J. P. & Bryant, J. (1979). Classification of peste des petits ruminants virus as the fourth member of the genus, Morbillivirus. Intervirology 2, 268–274.Google Scholar
Hamdy, F. M., Dardiri, A. H., Nduaka, O., Breese, S. S. & Ihemelandu, E. C. (1976). Etiology of the stomatitis pneumoenteritis complex in Nigerian dwarf goats. Canadian Journal of Comparative Medicine 40, 276–284.Google ScholarPubMed
Jacotot, H. (1931). Existe-t-il en Indochine plusieur virus pestique? Bulletin de la Société de pathologie exotique 24, 521–526.Google Scholar
Kataria, R. S., Shrivastava, S. N. & Majundar, S. S. (1977). Confirmation of rinderpest from samples of affected gums. Tropical Animal Health and Production 9, 232.CrossRefGoogle ScholarPubMed
Majiyaobe, K. A., Nawathe, D. R. & Abegunde, A. (1980). Diagnosis of PPR infection using immunoelectro-osmophoresis. Paper read at the first International workshop on PPR, Ibadan, Nigeria, 09 1980.Google Scholar
Makinde, A. A. & Majiyagbe, K. A. (1982). Serodiagnosis of Dermatophilus congolense infection by counterimmunoelectrophoresis. Research in Veterinary Science 33, 265–269.Google Scholar
Nicolle, M. & Abdil, Bey (1899). Etudes sur la peste bovine: premier mémoire. Annales de I'Institut Pasteur 13, 319–336.Google Scholar
Rossiter, P. B. & Mvshi, E. Z. (1980). Rapid detection of rinderpest virus antigen by counterimmunoelectrophoresis. Tropical Animal Health and Production 12, 209–216.CrossRefGoogle ScholarPubMed
Scott, G. R. & Brown, R. D. (1961). Rinderpest diagnosis with special reference to the agar gel double diffusion test. Bulletin of Epizootic Diseases in Africa 9, 83–125.Google Scholar
Scott, G. R. (1967). Diagnosis of Rinderpest. Food and Agriculture Organization Agricultural Studies No. 71, pp. 76–83. Rome: F.A.O.Google Scholar
Taylor, W. P. & Abegunde, A. (1979). Isolation of PPR vims from sheep and goats in Nigeria. Research in Veterinary Science 26, 94–96.CrossRefGoogle Scholar
Thanappa Pillai, M. & Abdul Khader, T. G. (1982). Study on the usefulness of infected gum scraping for confirming rinderpest in cattle by agar gel precipitation test. Cherion 11, 41–42.Google Scholar
Todd, C. & White, R. G. (1914). Experiments on Cattle Plague. Cairo: Government Press.Google Scholar
Van Saceghem, R. (1933). Le pulpe du tissue testiculaire dans la preparation du vaccin contre la peste bovine. Compte rendu des séances de la Société de biologie 113, 1539–1540.Google Scholar